Autometallographed metal content in digestive cells of winkles: a cost-effective screening tool for monitoring Cu and Zn pollution

Soto, Manu; Quincoces, Iñaki; Lekube, Xabier; Marigómez, Ionan

doi:10.1016/s0166-445x(97)00055-6

Cited by 10 publications

(10 citation statements)

References 42 publications

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“…Autometallography has been formerly reported to be a valuable technique for the localization and quantification of Cd, Cu, Zn, and Hg deposits in cellular compartments of marine and terrestrial animals (Hemelraad & Herwig 1988;Holwerda 1991;Marigo´mez et al 1996;Soto et al 1996Soto et al , 1998b. In the present study, metals weakly bound to proteins were readily visualized at the light microscope as BSD in the earthworm chloragocytes.…”

Section: Discussionmentioning

confidence: 54%

“…However, the individuals of the volcanically-active area, i.e., Furnas, presented a much higher BSD extent than the ones of the inactive volcanic area, i.e., Santa Maria. In fact, the BSD extent in the lysosomal compartment has been proposed as a metal exposure biomarker that reflects the levels of bioavailable metals in the environment (Soto & Marigo´mez 1997;Soto et al 1998bSoto et al , 1999Da Ros et al 2000;Porte et al 2001). According to a previous study, there is a higher bioavailability of zinc and cadmium in the soils of Furnas when compared with Santa Maria.…”

Section: Discussionmentioning

confidence: 99%

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Metal accumulation and apoptosis in the alimentary canal of Lumbricus terrestris as a metal biomarker

Amaral

Rodrigues

2005

Biometals

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The chloragogenous tissue and the intestinal epithelium of adult earthworms, Lumbricus terrestris, sampled from sites with and without volcanic activity in the Azores were submitted to hematoxylin/eosin staining, autometallography and TUNEL-test in order to quantify the radial thickness of both tissues, their relative abundance of metals and apoptosis levels. Metals were visualized, through light microscopy, as black silver deposits (BSD) mostly in the chloragogenous tissue. The lowest radial thickness values of both tissues were found in the active volcanic sites, as well as the highest BSD and apoptosis levels. The BSD extent in the chloragogenous tissue, semi-quantified by stereology, exhibited a positive correlation with the apoptosis levels and a negative one with the radial thickness of both tissues. Thus, the variation of the radial thickness of both tissues, but especially of the chloragogenous tissue, which could reflect different cellular turnover rates caused by exposure to metals, is suggested as a biomarker of effect for metal exposure in terrestrial worms inhabiting volcanic environments.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Metal accumulation and apoptosis in the alimentary canal of Lumbricus terrestris as a metal biomarker

Amaral

Rodrigues

2005

Biometals

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“…The more promising expectations concern the application of AMG, since a close parallel has been found between BSD in target cell compartments and the metal concentrations determined by AAS in the soft tissues of sentinel molluscs (Soto and Marigómez 1997a,b;Soto et al, 1998a). The amount of BSD revealed by AMG in the digestive lysosomes of winkles, Littorina littorea, was concluded to be an excellent indicator of exposure to Cu under laboratory conditions .…”

Section: Autometallographymentioning

confidence: 99%

“…Metal histochemistry and AMG are cost-effective techniques that may allow an accurate determination of the metal levels in biological samples and may reduce the influence of undesired natural variations Soto et al, 1996bSoto et al, , 1998aSoto and Marigómez 1997a,b). Until now, histochemistry and AMG have been applied in various investigations to demonstrate the association of metal levels in cells of molluscs with environmental levels of the metals (Hemelraad and Herwig 1988;Herwig et al, 1989Soto et al, 1996a,b;Giamberini et al, 1996a,b).…”

Section: Autometallographymentioning

confidence: 99%

Cellular and subcellular distribution of metals in molluscs

Marigómez

Soto

Cajaraville

et al. 2002

Microscopy Res & Technique

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419

202

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The cellular processes involved in metal metabolism in molluscs are reviewed, with emphasis on the contribution of microscopy (AMG, ARG, EPMA, and SIMS) to both basic research of metal cell biology and applied environmental research. In molluscs, metal uptake may occur by facilitated diffusion, active transport, or endocytosis, and can be enhanced by MT synthesis or formation of mineralized granules. In aquatic molluscs, gills constitute a key interface for dissolved metal uptake, where metals are bound to MT, incorporated into lysosomes, and released basally towards the blood plasma and circulating hemocytes. However, particulate metal uptake is mainly achieved via the digestive tract by endocytosis; further metals are transferred first to lysosomes and then to residual bodies, especially in the digestive cells of the digestive gland. Additionally, metals can be accumulated selectively in specific cell types. As ligands pools differ from cell to cell, different metals may be retained in different cell types. Class "a" metals are localized in cells with granules composed of carbonate, oxalate, phosphate, and sulfate (oxygen donors), whereas "b" metals are associated with those cell types rich in sulfur and nitrogen ligands (sulfur donors). In molluscs, oxygen donors occur in connective tissue calcium cells and basophilic cells, whereas sulfur donors are present in digestive cells, podocytes, nephrocytes, and rhogocytes. Hemocytes, which constitute the most relevant system for metal transport between tissues, move around the body and may penetrate tissues and remove metals from the inner medium to be accumulated in lysosomes as nondigested products. Rhogocytes also participate in metal mobilization, accumulation, and release. The assessment of metal levels in target cells of sentinel molluscs by microscopic techniques provides an early-warning measure, with promising applications as an exposure biomarker for environmental monitoring programs.

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“…In previous work, Soto et al (1997b) demonstrated that the semiquantitative estimation of BSD in digestive cells of winkles was a useful tool to monitor Cu and Zn pollution. Additionally, Soto et al (1996), applying autometallography, found target cellular sites for Cu, Zn, and Cd in various cell types of gills and in digestive cells of mussels.…”

Section: Introductionmentioning

confidence: 99%

BSD Extent, an Index for Metal Pollution Screening Based on the Metal Content within Digestive Cell Lysosomes of Mussels as Determined by Autometallography

Soto

Marigómez

1997

Ecotoxicology and Environmental Safety

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Autometallographed metal content in digestive cells of winkles: a cost-effective screening tool for monitoring Cu and Zn pollution

Cited by 10 publications

References 42 publications

Metal accumulation and apoptosis in the alimentary canal of Lumbricus terrestris as a metal biomarker

Metal accumulation and apoptosis in the alimentary canal of Lumbricus terrestris as a metal biomarker

Cellular and subcellular distribution of metals in molluscs

BSD Extent, an Index for Metal Pollution Screening Based on the Metal Content within Digestive Cell Lysosomes of Mussels as Determined by Autometallography

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