Automated Mass Spectrometry–Based Functional Assay for the Routine Analysis of the Secretome

Wetie, Armand G. Ngounou; Sokolowska, Izabela; Woods, Alisa G.; Wormwood, Kelly L.; Dao, Su; Patel, Sapan J.; Clarkson, Bayard D.; Darie, Costel C.

doi:10.1177/2211068212454738

Cited by 52 publications

(39 citation statements)

References 83 publications

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“…The gels were then stained with Coomassie blue, and the most intense bands were excised and digested with trypsin. Digested proteins were then analyzed by nanoliquid chromatography tandem mass spectrometry (nano LC-MS/MS) using a Nano Acquity UPLC coupled to a QTOF Micro mass spectrometer, according to published procedures [13][14][15][16][17][18][19]. The raw data were processed using Protein Lynx Global Server (PLGS, version 2.4) and the resulting pkl files were submitted to Mascot database search [13][14][15][16][17][18][19].…”

Section: Methodsmentioning

confidence: 99%

What’s in Your Yogurt? A Proteomic Investigation

Gooding

Yeaton²,

Wallace³

et al. 2014

Mod Chem Appl

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Different kinds of yogurt contain different percentages of proteins. However, do they also contain different types of proteins? To answer to this question, we investigated the protein pattern in the soluble fraction of various yogurts. We found that many yogurts contain mostly two main bands: a ~20 kDa band and a ~15 kDa band. Proteomics analysis of these two bands led us to conclude that the 20 kDa band corresponds to lactoglobulin isoforms and the 15 kDa band corresponds to casein isoforms.

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Section: Methodsmentioning

confidence: 99%

What’s in Your Yogurt? A Proteomic Investigation

Gooding

Yeaton²,

Wallace³

et al. 2014

Mod Chem Appl

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“…Due to high-performing MS instruments, simplified analytical workflows and versatile data analysis, mass spectrometry is applicable to almost every area of the life sciences and potentially even far beyond [51–54]. The two most common ionization methods are electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI).…”

Section: Reviewmentioning

confidence: 99%

“…For visualization of protein bands, the gel can be stained either with the dye coomassie blue or silver stained or by fluorescently labeling the samples before 2-DE. For mass spectrometry analysis, gel bands are removed, and undergo several other steps to generate peptides in bottom-up and shotgun proteomics [51, 53, 67, 68]. Compared to LC/MS, 2-DE methods are more reproducible and robust.…”

Section: Reviewmentioning

confidence: 99%

Mass spectrometry for the detection of potential psychiatric biomarkers

et al. 2013

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The search for molecules that can act as potential biomarkers is increasing in the scientific community, including in the field of psychiatry. The field of proteomics is evolving and its indispensability for identifying biomarkers is clear. Among proteomic tools, mass spectrometry is the core technique for qualitative and quantitative identification of protein markers. While significant progress has been made in the understanding of biomarkers for neurodegenerative diseases such as Alzheimer’s disease, multiple sclerosis and Parkinson’s disease, psychiatric disorders have not been as extensively investigated. Recent and successful applications of mass spectrometry-based proteomics in fields such as cardiovascular disease, cancer, infectious diseases and neurodegenerative disorders suggest a similar path for psychiatric disorders. In this brief review, we describe mass spectrometry and its use in psychiatric biomarker research and highlight some of the possible challenges of undertaking this type of work. Further, specific examples of candidate biomarkers are highlighted. A short comparison of proteomic with genomic methods for biomarker discovery research is presented. In summary, mass spectrometry-based techniques may greatly facilitate ongoing efforts to understand molecular mechanisms of psychiatric disorders.

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“…MS is a method that can reliably and comprehensively identify proteins and putative biomarkers [4,5,[12][13][14][15][16][17][18][19][20][21][22]. MS is already in use for newborn screening for several other disorders, including phenylketonuria, congenital hypothyroidism and cystic fibrosis [23].…”

Section: Introductionmentioning

confidence: 99%