Automated gas chromatographic assay for amlodipine in plasma and gingival crevicular fluid

Monkman, S. C.; Ellis, J. S.; Cholerton, S.; Thomason, JM; Seymour, R. A.; Idle, J R

doi:10.1016/0378-4347(95)00526-9

Cited by 51 publications

(26 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other methods such as capillary electrophoresis (LOQ = 5.0 ng/ml; RT = 9.8 min) [5], and gas chromatography (LOQ = 2.5 ng/ml; RT>9.0 min) [6] were also used to detect amlodipine plasma levels.…”

Section: Resultsmentioning

confidence: 99%

Amlodipine bioequivalence study: quantification by liquid chromatography coupled to tandem mass spectrometry

Carvalho

Oliveira

Mendes³

et al. 2001

Biopharm & Drug Disp

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Amlodipine bioequivalence study: quantification by liquid chromatography coupled to tandem mass spectrometry

Carvalho

Oliveira

Mendes³

et al. 2001

Biopharm & Drug Disp

View full text Add to dashboard Cite

show abstract

“…Monkman SC, Ellis JS, Cholerton S, Thomason JM, Seymour RA, et al (1996) Automated gas chromatographic assay for amlodipine in plasma and gingival crevicular fluid. J Chromatogr B Biomed Appl 678: 360-364.…”

Section: Referencesmentioning

confidence: 99%

“…The plasma elimination half-life of Levamlodipine has been found to be 31 ± 13 hrs. Different methods for the determination of Amlodipine in biological fluids, such as gas chromatography (GC) [1] or liquid chromatography (LC) [2][3][4] or mass spectrometric analysis [5][6][7], previously reported methods [8][9][10][11][12] either had long retention time (10-15min) or suffered from low sensitivity and in some cases required large sample injection volumes. Hence the main objective of this work was to develop a simple, sensitive, rapid and reliable mass spectrometry method for the quantification of S-amlodipine in human plasma.…”

Section: Introductionmentioning

confidence: 99%

Determination of S-amlodipine in Human Plasma Using Tizanidine as Internal Standard by Lc/Ms/Ms Method

Durga¹

2015

J Bioanal Biomed

View full text Add to dashboard Cite

Table 2: Result of stability, dilution integrity, matrix effect and ruggedness.CPD as an anticoagulant in polypropylene tubes. The stability ranged from 99.1% to 101.3%, which are within limits (Table 2). Stock solution stability at room temperatureStock solution stability at room temperature was performed by storing S-amlodipine and internal standard (Tizanidine) stock solutions at room temperature. The evaluation of stability was done by assaying six replicate injections of appropriately prepared dilutions of stored stock aliquot solutions of S-amlodipine and internal standard (Tizanidine) against six replicate injection of appropriately prepared dilution from fresh stock solutions of S-amlodipine and internal standard (Tizanidine) .The stock solution of S-amlodipine and internal standard (Tizanidine) was found to be stable, when stored at room temperature. The percent stability of the stock solution stability was 97.5% and 103.3% respectively, which are within limits (Table 2). Dilution integrityDilution Integrity was determined by assaying four replicates QCs spiked approximately two times the concentration of ULOQ. Samples were diluted by an appropriate factor (two) prior to extraction, against freshly spiked calibration standard samples. The accuracy for two times diluted concentration was 101.9% and four times diluted concentration were 101.2% for S-amlodipine .The precision for two times diluted concentration were 4.0% and four times diluted concentration were 2.8% for S-amlodipine , which are within limits (Table 2). Matrix effectMatrix effect was calculated by spiking analyte and IS at LQC and HQC levels into each of blank plasma extracts from six different batches of matrix respectively and analyzed in duplicate against six replicate injections of aqueous samples at low and high QCs samples. At low and QCs samples, the % coefficient of variation for matrix factor was found to be 9.35% and 6.84%, respectively for S-amlodipine which is within limits (Table 2). RuggednessThe ruggedness of the extraction procedure and chromatographic method was evaluated by analysis of a batch of six sets of quality control samples and a set of calibration standards using different column (same type) by another analyst. The within batch accuracy ranged from 107.1% to111.4% and within batch precision ranged from 1.8% to 4.7% for S-amlodipine .The results indicated that the batch met the acceptance criteria of linearity, precision and accuracy data of the quality control samples, which are within limits (Table 2). Stock solution stability at refrigerated temperature (4.0 days)Stock solution stability at refrigerated temperature was performed by S-amlodipine and internal standard (Tizanidine) stock solutions at refrigerated temperature. The evaluation of stability was done by assaying six replicates injections of appropriately prepared dilution from stored aliquot solutions of S-amlodipine and internal standard (Tizanidine) against six replicate injection of appropriately prepared dilution from fresh stock solutions of S-am...

show abstract

“…Several methods, based on various analytical techniques, have been described in the literature for the determination of amlodipine besylate in pure form as well as in chemistry laboratory formulations, pharmaceutical formulations, and biological fluids. They include spectrophotometric methods [17,18], spectrofluorometric methods [19], high-performance thin-layer chromatography [20], high-performance liquid chromatography (HPLC) [21,22], liquid chromatography (LC) [23], gas chromatography (GC) [24], capillary electrophoresis [25], flow injection analysis [26], and enzyme-linked immunosorbent assay [27].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Biosensor for the Determination of Amlodipine Besylate Based on Gelatin–Polyaniline Iron Oxide Biocomposite Film

et al. 2018

View full text Add to dashboard Cite

Abstract:In the present study, a new biosensor based on lipase from Candida rugosa (CRL) was developed for amlodipine besylate drug (AMD) with biodegradable material using a mixture of polyaniline iron oxide and gelatin. Polyaniline/Fe 2 O 3 (PANI@Fe 2 O 3 ) was prepared by a chemical polymerization method in a medium of ammonium persulfate as an oxidant and characterized by employing Scanning Electron Microscopy (SEM), Fourier Transform Infrared (FTIR), and Ultra-violet (UV) spectroscopy. The purified enzyme was entrapped in the biocomposite matrix film with the aid of a glutaraldehyde cross-linking reagent to establish the immobilization of the lipase. The principle of the biosensor is based on the electrochemical properties of amlodipine besylate (AMD), which were studied for the first time using the cyclic voltammetric method. The cathodic behavior of AMD was measured on the irreversible reduction signal at −0.185 V versus Ag/AgCl at pH 7.4 and 30 • C in a phosphate alkaline buffer.

show abstract

Automated gas chromatographic assay for amlodipine in plasma and gingival crevicular fluid

Cited by 51 publications

References 12 publications

Amlodipine bioequivalence study: quantification by liquid chromatography coupled to tandem mass spectrometry

Amlodipine bioequivalence study: quantification by liquid chromatography coupled to tandem mass spectrometry

Determination of S-amlodipine in Human Plasma Using Tizanidine as Internal Standard by Lc/Ms/Ms Method

Electrochemical Biosensor for the Determination of Amlodipine Besylate Based on Gelatin–Polyaniline Iron Oxide Biocomposite Film

Contact Info

Product

Resources

About