2014
DOI: 10.1098/rsif.2014.0386
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Abstract: ResearchCite this article: Baker RM, Brasch ME, Manning ML, Henderson JH. 2014 Automated, contour-based tracking and analysis of cell behaviour over long time scales in environments of varying complexity and cell density. J. R. Soc. Understanding single and collective cell motility in model environments is foundational to many current research efforts in biology and bioengineering. To elucidate subtle differences in cell behaviour despite cell-to-cell variability, we introduce an algorithm for tracking large n… Show more

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Cited by 27 publications
(35 citation statements)
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“…Time-lapse videos consisting of 288 frames per video, representing 24 h of real time, were then analyzed by Automated Contour-based Tracking for In Vitro Environments, ACT IV E, a computational algorithm we recently introduced for tracking large numbers of cells for long time periods that provides qualitative and quantitative analysis of cell motility [40]. ACT IV E was used to analyze cell motility in each time-lapse video and output cell motility tracks, diffusion plots, average and decomposed mean square displacement (MSD) plots and metrics, and average velocity plots and metrics (Supplemental Method 5).…”
Section: Methodsmentioning
confidence: 99%
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“…Time-lapse videos consisting of 288 frames per video, representing 24 h of real time, were then analyzed by Automated Contour-based Tracking for In Vitro Environments, ACT IV E, a computational algorithm we recently introduced for tracking large numbers of cells for long time periods that provides qualitative and quantitative analysis of cell motility [40]. ACT IV E was used to analyze cell motility in each time-lapse video and output cell motility tracks, diffusion plots, average and decomposed mean square displacement (MSD) plots and metrics, and average velocity plots and metrics (Supplemental Method 5).…”
Section: Methodsmentioning
confidence: 99%
“…A number of recent breakthroughs in the area of cytocompatible SMPs [3239] have enabled application of SMP on-command functionality in cell culture application. Although SMPs have not previously been applied in the study of cell motility and polarity switching, we have recently demonstrated the feasibility of employing SMPs in the study of cell motility in a 2D system [40]. …”
Section: Introductionmentioning
confidence: 99%
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“…For example, models may be designed to allow cell division but prevent cell fusion [15]. Most model evolution methods evolve the contours of the cells [16][17][18]. Other approaches rely on topology-constrained level set methods [1,19].…”
Section: Previous Workmentioning
confidence: 99%
“…Information on lineage or ancestry is an important aspect of tracking multiple spawning objects. For example, in (biological) cell tracking, information on a cell's lineage is important to the analyis of cell behavior [8], [21]- [23]. For space situational awareness, information on the ancestry of debris is important to the analysis of fragmentation events [24]- [26]; moreover, country of origin and launch site, information required to add a space object to the United States Strategic Command (USSTRATCOM) catalog [27], can be derived from ancestry information.…”
Section: Introductionmentioning
confidence: 99%