Autoinducer-2 Facilitates Pseudomonas aeruginosa PAO1 Pathogenicity in Vitro and in Vivo

Li, Hongdong; Li, Xingyuan; Song, Chao; Zhang, Yunhui; Wang, Zhengli; Liu, Zhenqiu; Wang, Hong; Yu, Jialin

doi:10.3389/fmicb.2017.01944

Cited by 36 publications

(28 citation statements)

References 43 publications

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“…For example, AI-2 produced by Enterococcus faecalis leads to enhanced aggregation and biofilm formation by E. coli as well as coaggregation of these two species 14 , 41 . Patients of cystic fibrosis and other disease conditions are often co-infected by P. aeruginosa and AI-2-producing bacterial species such as Staphylococcus aureus, Klebsiella pneumoniae and Streptococcus mitis 17 , 18 , 40 . The observation that PctA and TlpQ facilitate the response of P. aeruginosa toward AI-2 may explain why AI-2 in cystic fibrosis lungs enhances the virulence of P. aeruginosa 16 – 18 , 40 .…”

Section: Discussionmentioning

confidence: 99%

Sensing of autoinducer-2 by functionally distinct receptors in prokaryotes

et al. 2020

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Autoinducer-2 (AI-2) is a quorum sensing signal that mediates communication within and between many bacterial species. However, its known receptors (LuxP and LsrB families) are not found in all the bacteria capable of responding to this signaling molecule. Here, we identify a third type of AI-2 receptor, consisting of a dCACHE domain. AI-2 binds to the dCACHE domain of chemoreceptors PctA and TlpQ of Pseudomonas aeruginosa, thus inducing chemotaxis and biofilm formation. Boron-free AI-2 is the preferred ligand for PctA and TlpQ. AI-2 also binds to the dCACHE domains of histidine kinase KinD from Bacillus subtilis and diguanylate cyclase rpHK1S-Z16 from Rhodopseudomonas palustris, enhancing their enzymatic activities. dCACHE domains (especially those belonging to a subfamily that includes the AI-2 receptors identified in the present work) are present in a large number of bacterial and archaeal proteins. Our results support the idea that AI-2 serves as a widely used signaling molecule in the coordination of cell behavior among prokaryotic species.

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Section: Discussionmentioning

confidence: 99%

Sensing of autoinducer-2 by functionally distinct receptors in prokaryotes

et al. 2020

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“…There might be something, which was commonly secreted by Gramnegative bacteria, especially in E. coli, and it could affect the development of CRPA. In recent years, a new regulating mechanism defined as quorum sensing was found to play an important role in biofilm formation [29] The signaling molecules of quorum sensing mainly contain two parts, oligopeptides are commonly secreted by Gram-positive bacteria, while N-acyl-L-homoserine lactones (AHLs) are commonly secreted by Gramnegative bacteria [30]. AHLs are one of the most wellcharacterized signaling molecules, and it was called the talking language in Gram-negative bacteria [31].…”

Section: Discussionmentioning

confidence: 99%

Risk factors for carbapenem-resistant Pseudomonas aeruginosa infection or colonization in a Chinese teaching hospital

Zhang¹,

Cui

Wang

et al. 2018

J Infect Dev Ctries

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Introduction: Carbapenem-resistant Pseudomonas aeruginosa (CRPA) is rapidly emerging as a life-threatening nosocomial infection. In this study, we aimed to identify risk factors, especially antibiotic use and co-carriage with other bacteria for CRPA infection or colonization. Methodology: A retrospective study was conducted in the First Affiliated Hospital of Xi’an Jiaotong University, which involved a cohort of patients with Pseudomonas aeruginosa infection or colonization from January 2014 to June 2016. Univariate analysis and multivariate analysis were performed to estimate the risk factors of CRPA occurrence. Results: Eight hundred and eighty-eight patients were included in the study. More than 50% of the risk factors were associated with CRPA infection or colonization according to univariate analysis (P < 0.05), such as invasive procedures, co-carriage with Gram-negative pathogens, and prior treatment with some antibiotics. However, only prior exposure to carbapenems (OR: 8.005; CI:4.507-14.217, P<0.001), the days of carbapenems treatment (OR: 1.190; CI: 1.073-1.272; P < 0.001), and co-carriage with Escherichia coli (OR: 1.824; CI: 1.005-3.310, P = 0.048) were considered independent risk factors by multivariate analysis. A higher mortality was found among patients with CRPA infection or colonization (P < 0.05). Conclusions: Risk factors for CRPA infection or colonization were prior exposure to carbapenems, the days of carbapenems treatment, and co-carriage with Escherichia coli. The prevalence of CRPA could be influenced by Gram-negative pathogens, especially in Escherichia coli, and it need more researches. Moreover, restrictions in the clinical use of carbapenems should be taken into account.

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“…Previous research has shown an interplay between quorum sensing and biofilm formation [42,43]. For instance, AI-2 production or uptake has been shown to influence E. coli biofilm formation via an effect on flagellar motion and motility [44], as well as biofilm formation by Pseudomonas aeruginosa [45] and S. enterica serovar Typhimurium [46]. K. pneumoniae forms biofilms on various surfaces, where cell attachment is dependent on the production of exopolysaccharides and adhesive proteins such as type 3 fimbriae [47][48][49][50].…”

Section: Assessment Of Biofilm Formation By a K Pneumoniae δLuxs Mutantmentioning

confidence: 99%

Investigation of LuxS-mediated quorum sensing in Klebsiella pneumoniae

Chen

Wilksch

Liu

et al. 2020

Journal of Medical Microbiology

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Introduction. Autoinducer-2 (AI-2) quorum sensing is a bacterial communication system that responds to cell density. The system requires luxS activity to produce AI-2, which can regulate gene expression and processes such as biofilm formation. Aim. To investigate the role of luxS in biofilm formation and gene expression in the nosocomial pathogen Klebsiella pneumoniae . Methodology. A ΔluxS gene deletion was made in K. pneumoniae KP563, an extensively drug-resistant isolate. AI-2 production was assessed in wild-type and ΔluxS strains grown in media supplemented with different carbohydrates. Potential roles of luxS in biofilm formation were investigated using a microtiter plate biofilm assay and scanning electron microscopy. Quantitative RT-PCR evaluated the expression of lipopolysaccharide (wzm and wbbM), polysaccharide (pgaA), and type 3 fimbriae (mrkA) synthesis genes in wild-type and ΔluxS mutant biofilm extracts. Results. AI-2 production was dependent on the presence of luxS. AI-2 accumulation was highest during early stationary phase in media supplemented with glucose, sucrose or glycerol. Changes in biofilm architecture were observed in the ΔluxS mutant, with less surface coverage and reduced macrocolony formation; however, no differences in biofilm formation between the wild-type and ΔluxS mutant using a microtiter plate assay were observed. In ΔluxS mutant biofilm extracts, the expression of wzm was down-regulated, and the expression of pgaA, which encodes a porin for poly-β−1,6-N-acetyl-d-glucosamine (PNAG) polysaccharide secretion, was upregulated. Conclusion. Relationships among AI-2-mediated quorum sensing, biofilm formation and gene expression of outer-membrane components were identified in K. pneumoniae . These inter-connected processes could be important for bacterial group behaviour and persistence.

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Autoinducer-2 Facilitates Pseudomonas aeruginosa PAO1 Pathogenicity in Vitro and in Vivo

Cited by 36 publications

References 43 publications

Sensing of autoinducer-2 by functionally distinct receptors in prokaryotes

Sensing of autoinducer-2 by functionally distinct receptors in prokaryotes

Risk factors for carbapenem-resistant Pseudomonas aeruginosa infection or colonization in a Chinese teaching hospital

Investigation of LuxS-mediated quorum sensing in Klebsiella pneumoniae

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