Autofluorescence of normal and malignant bronchial tissue

Hung, Jaclyn Y.; Lam, Stephen; LeRiche, Jean; Palcic, Branko

doi:10.1002/lsm.1900110203

Cited by 356 publications

(199 citation statements)

References 11 publications

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“…The ratio is higher for tumour than for any other of the studied organs. This is because of a decrease of the autofluorescence intensity in the tumour, as has been shown previously (Ankerst et al, 1984;Hung et al, 1991).…”

Section: Materials and Methods Chemicalssupporting

confidence: 69%

Laser-induced fluorescence studies of the biodistribution of carotenoporphyrins in mice

Nilsson

Johansson²,

Svanberg³

et al. 1997

Br J Cancer

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Summary The biodistribution of two recently developed tumour markers, trimethylated (CP(Me)3) and trimethoxylated (CP(OMe)3) carotenoporphyrin, was investigated by means of laser-induced fluorescence (LIF) after i.v. injection into 38 tumour-bearing (MS-2 fibrosarcoma) female Balb/c mice. At 3, 24, 48 or 96 h after administration, the carotenoporphyrin fluorescence was measured in tumoral and peritumoral tissue, as well as in the abdominal, thoracic and cranial cavities. The fluorescence was induced by a nitrogen laser-pumped dye laser, emitting light at 425 nm, and analysed by a polychromator equipped with an image-intensified CCD camera. The fluorescence was evaluated at 490, 655 and 720 nm: the second and third wavelengths represent the carotenoporphyrin (CP)-related peaks, whereas the first one is close to the peak of the tissue autofluorescence. The tumour and the liver were the two tissue types showing the strongest carotenoporphyrin-related fluorescence, whereas the cerebral cortex and muscle consistently exhibited weak substance-related fluorescence. In most tissue types, the fluorescence intensities decreased over time. A few exceptions were observed, notably the liver, in which the intensity remained remarkably constant over the time period investigated.

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Section: Materials and Methods Chemicalssupporting

confidence: 69%

Laser-induced fluorescence studies of the biodistribution of carotenoporphyrins in mice

Nilsson

Johansson²,

Svanberg³

et al. 1997

Br J Cancer

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“…Thus the difference between normal and cancerous tissues can be investigated based on their autofluorescence spectra. However, this approach is limited by that autofluorescence spectrum features exhibit less specific differences and the origin of the contrasts mechanisms are not clearly identified [18,19].…”

Section: Introductionmentioning

confidence: 99%

Autofluorescence Imaging and Spectroscopy of Human Lung Cancer

et al. 2016

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Lung cancer is one of the most common cancers, with high mortality rate worldwide. Autofluorescence imaging and spectroscopy is a non-invasive, label-free, real-time technique for cancer detection. In this study, lung tissue sections excised from patients were detected by laser scan confocal microscopy and spectroscopy. The autofluorescence images demonstrated the cellular morphology and tissue structure, as well as the pathology of stained images. Based on the spectra study, it was found that the majority of the patients showed discriminating fluorescence in tumor tissues from normal tissues. Therefore, autofluorescence imaging and spectroscopy may be a potential method for aiding the diagnosis of lung cancer.

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“…The principle of this diagnostic procedure is that normal bronchial tissue emits green auto fluorescence (500-600 nm) excited by blue light, while tumor or dysplasia lack green auto fluorescence because of differences in tissue architecture and auto fluorescence fluorophores (8)(9)(10). Although the value of fluorescence bronchoscopy in detecting early lung cancer/pre-invasive lesions has been evaluated in different clinical and research settings, its role in the routine follow-up of PDT treated early lung cancer has not been extensively evaluated.…”

Section: Introductionmentioning

confidence: 99%