Autofluorescence lifetime flow cytometry with time‐correlated single photon counting
Kayvan Samimi,
Ojaswi Pasachhe,
Emmanuel Contreras Guzman
et al.
Abstract:Autofluorescence lifetime imaging microscopy (FLIM) is sensitive to metabolic changes in single cells based on changes in the protein-binding activities of the metabolic co-enzymes NAD(P)H. However, FLIM typically relies on time-correlated single-photon counting (TCSPC) detection electronics on laser-scanning microscopes, which are expensive, low-throughput, and require substantial post-processing time for cell segmentation and analysis. Here, we present a fluorescence lifetime-sensitive flow cytometer that of… Show more
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