Author response: Splicing in a single neuron is coordinately controlled by RNA binding proteins and transcription factors

Thompson, Morgan N.; Bixby, Ryan; Dalton, Robert; Vandenburg, Alexa; Calarco, John A.; Norris, Adam

doi:10.7554/elife.46726.023

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To prove this hypothesis, I constructed a dual-color splicing reporter (Norris et al, 2014;Thompson et al, 2019) of the skipping of exon 5 in fust-1 (Figure S4C), in which no skipping gives GFP expression while skipping of exon 5 results in mCherry expression. As expected, two colors were co-expressed in almost all the neurons in wild-type strain (Figure 5D), suggesting that exon-skipping of exon 5 is happening in all the neurons.…”

Section: An Autoregulation Loop In Fust-1mentioning

confidence: 99%

An autoregulation loop infust-1for circular RNA regulation inCaenorhabditis elegans

Cao

2021

Preprint

View full text Add to dashboard Cite

Circular RNAs (circRNAs) are always expressed tissue-specifically, suggestive of specific factors that regulate their biogenesis. Here, taking advantage of available mutation strains of RNA binding proteins (RBPs) in Caenorhabditis elegans, I performed a screening of circRNA regulation in thirteen conserved RBPs. Among them, loss of FUST-1, the homolog of FUS (Fused in Sarcoma), caused downregulation of multiple circRNAs. By rescue experiments, I confirmed FUST-1 as a circRNA regulator. Further, I showed that FUST-1 regulates circRNA formation without affecting the levels of the cognate linear mRNAs. When recognizing circRNA pre-mRNAs, FUST-1 can affect both exon-skipping and circRNA in the same genes. Moreover, I identified an autoregulation loop in fust-1, where FUST-1, isoform a promotes the skipping of exon 5 of its own pre-mRNA, which produces FUST-1, isoform b with different N-terminal sequences. FUST-1, isoform a is the functional isoform in circRNA regulation. Although FUST-1, isoform b has the same functional domains as isoform a, it cannot regulate either exon-skipping or circRNA formation.

show abstract

Section: An Autoregulation Loop In Fust-1mentioning

confidence: 99%

An autoregulation loop infust-1for circular RNA regulation inCaenorhabditis elegans

Cao

2021

Preprint

View full text Add to dashboard Cite

show abstract

Author response: Splicing in a single neuron is coordinately controlled by RNA binding proteins and transcription factors

Cited by 1 publication

References 0 publications

An autoregulation loop infust-1for circular RNA regulation inCaenorhabditis elegans

An autoregulation loop infust-1for circular RNA regulation inCaenorhabditis elegans

Contact Info

Product

Resources

About