Authentication of “mono-breed” pork products: Identification of a coat colour gene marker in Cinta Senese pigs useful to this purpose

Scotti, Emilio; Gallo, M.; Costa, Leonardo Nanni; Dall’Olio, Stefania

doi:10.1016/j.livsci.2015.12.007

Cited by 30 publications

(27 citation statements)

References 30 publications

(37 reference statements)

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“…Thus, full characterization of KIT alleles was beyond the scope of this work. Nevertheless, the KIT g. 43597545C>T (g.41488472 in Sscrofa11.1) SNP was included in the panel, since it had been previously associated to the belted phenotype [ 68 ]. This SNP was proposed as suitable marker for differentiation of Cinta Senese pigs from other non-belted breeds and in agreement our results show a very high frequency of allele T in Cinta Senese (0.98), while most other breeds are fixed or almost fixed for the C allele.…”

Section: Resultsmentioning

confidence: 99%

Diversity across major and candidate genes in European local pig breeds

Muñoz¹,

Bozzi

García³

et al. 2018

PLoS ONE

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The aim of this work was to analyse the distribution of causal and candidate mutations associated to relevant productive traits in twenty local European pig breeds. Also, the potential of the SNP panel employed for elucidating the genetic structure and relationships among breeds was evaluated. Most relevant genes and mutations associated with pig morphological, productive, meat quality, reproductive and disease resistance traits were prioritized and analyzed in a maximum of 47 blood samples from each of the breeds (Alentejana, Apulo-Calabrese, Basque, Bísara, Majorcan Black, Black Slavonian (Crna slavonska), Casertana, Cinta Senese, Gascon, Iberian, Krškopolje (Krškopoljski), Lithuanian indigenous wattle, Lithuanian White Old Type, Mora Romagnola, Moravka, Nero Siciliano, Sarda, Schwäbisch-Hällisches Schwein (Swabian Hall pig), Swallow-Bellied Mangalitsa and Turopolje). We successfully analyzed allelic variation in 39 polymorphisms, located in 33 candidate genes. Results provide relevant information regarding genetic diversity and segregation of SNPs associated to production and quality traits. Coat color and morphological trait-genes that show low level of segregation, and fixed SNPs may be useful for traceability. On the other hand, we detected SNPs which may be useful for association studies as well as breeding programs. For instance, we observed predominance of alleles that might be unfavorable for disease resistance and boar taint in most breeds and segregation of many alleles involved in meat quality, fatness and growth traits. Overall, these findings provide a detailed catalogue of segregating candidate SNPs in 20 European local pig breeds that may be useful for traceability purposes, for association studies and for breeding schemes. Population genetic analyses based on these candidate genes are able to uncover some clues regarding the hidden genetic substructure of these populations, as the extreme genetic closeness between Iberian and Alentejana breeds and an uneven admixture of the breeds studied. The results are in agreement with available knowledge regarding breed history and management, although largest panels of neutral markers should be employed to get a deeper understanding of the population’s structure and relationships.

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Section: Resultsmentioning

confidence: 99%

Diversity across major and candidate genes in European local pig breeds

Muñoz¹,

Bozzi

García³

et al. 2018

PLoS ONE

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show abstract

“…On the other hand, this heterogeneity should be managed to create phenotypically uniform populations that might better match the standard traits defined by their herd books, useful for acquiring a specific identity for these pig genetic resources and making possible the application of DNA‐based systems for the authentication of their products (Fontanesi ; Fontanesi et al . ).…”

Section: Frequencies Of Melanocortin 1 Receptor (Mc1r) and Nuclear Rementioning

confidence: 97%

Signatures of de‐domestication in autochthonous pig breeds and of domestication in wild boar populations from MC1R and NR6A1 allele distribution

et al. 2019

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Autochthonous pig breeds are usually reared in extensive or semi-extensive production systems that might facilitate contact with wild boars and, thus, reciprocal genetic exchanges. In this study, we analysed variants in the melanocortin 1 receptor (MC1R) gene (which cause different coat colour phenotypes) and in the nuclear receptor subfamily 6 group A member 1 (NR6A1) gene (associated with increased vertebral number) in 712 pigs of 12 local pig breeds raised in Italy (Apulo-Calabrese, Casertana, Cinta Senese, Mora Romagnola, Nero Siciliano and Sarda) and south-eastern European countries (Kr skopolje from Slovenia, Black Slavonian and Turopolje from Croatia, Mangalitsa and Moravka from Serbia and East Balkan Swine from Bulgaria) and compared the data with the genetic variability at these loci investigated in 229 wild boars from populations spread in the same macro-geographic areas. None of the autochthonous pig breeds or wild boar populations were fixed for one allele at both loci. Domestic and wild-type alleles at these two genes were present in both domestic and wild populations. Findings of the distribution of MC1R alleles might be useful for tracing back the complex genetic history of autochthonous breeds. Altogether, these results indirectly demonstrate that bidirectional introgression of wild and domestic alleles is derived and affected by the human and naturally driven evolutionary forces that are shaping the Sus scrofa genome: autochthonous breeds are experiencing a sort of 'dedomestication' process, and wild resources are challenged by a 'domestication' drift. Both need to be further investigated and managed.

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“…Alves et al analysed porcine mitochondrial DNA and identified SNPs specific to Iberian and Duroc pigs [36]. Another SNP in a nuclear gene encoding v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog ( KIT ) was found to be useful for authentication of meat products obtained from Cinta Senese pigs reared exclusively in Italy [37]. A 96-plex SNP genotyping assay using markers selected from the PorcineSNP60 BeadChip was shown to be a powerful tool for identification of traditional and commercial breeds of domestic pigs from the United Kingdom [38].…”

Section: Discussionmentioning

confidence: 99%

STR Profiling for Discrimination between Wild and Domestic Swine Specimens and between Main Breeds of Domestic Pigs Reared in Belarus

et al. 2016

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A panel comprising 16 short tandem repeats (STRs) and a gender-specific amelogenin marker was worked out and tested for robustness in discrimination between wild and domestic swine subspecies encountered in Europe, between regional populations of wild boars and between main breeds of domestic pigs reared in Belarus. The STR dataset comprised 310 wild boars, inhabiting all administrative regions of Belarus, and 313 domestic pigs, representing three local and three cosmopolitan lines. Additionally, a total of 835 wild boars were genotyped for the presence of melanocortin 1 receptor (MC1R) alleles specific for domestic pigs. Correctness of assignment of STR profiles to appropriate populations was measured by log-likelihood ratios (log-LRs). All samples were correctly identified as wild boars or domestic pigs with average log-LR of 42.4 (LR = 2.6×1018). On the other hand, as many as 50 out of 835 (6.0%) genotyped wild boars from Belarus possessed MC1R alleles specific to domestic pigs, demonstrating supremacy of our STR profiling system over traditional differentiation between wild boars and domestic pigs, based on single binary markers. Mean log-LRs for allocation of wild boars to their regions of origin and of domestic pigs to appropriate breeds were 2.3 (LR = 9.7) and 13.4 (LR = 6.6×105), respectively. Our results demonstrate the developed STR profiling system to be a highly efficient tool for differentiation between wild and domestic swine subspecies and between diverse breeds of domestic pigs as well as for verification of genetic identity of porcine specimens for the purpose of forensic investigations of wildlife crimes, assurance of veterinary public health, parentage control in animal husbandry, food safety management and traceability of livestock products.

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Authentication of “mono-breed” pork products: Identification of a coat colour gene marker in Cinta Senese pigs useful to this purpose

Cited by 30 publications

References 30 publications

Diversity across major and candidate genes in European local pig breeds

Diversity across major and candidate genes in European local pig breeds

Signatures of de‐domestication in autochthonous pig breeds and of domestication in wild boar populations from MC1R and NR6A1 allele distribution

STR Profiling for Discrimination between Wild and Domestic Swine Specimens and between Main Breeds of Domestic Pigs Reared in Belarus

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