Authentication of honeys of different floral origins <i>via</i> high-performance thin-layer chromatographic fingerprinting

Locher, Cornelia; Neumann, Jonas; Sostaric, Tomislav

doi:10.1556/1006.2017.30.1.8

Cited by 35 publications

(32 citation statements)

References 14 publications

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“…A standard stock solution of gallic acid (20 µg/mL) in methanol and a reference solution of 0.5 mg/mL of 4,5,7-trihydroxyflavanone in methanol were prepared. A mixture of toluene: ethyl acetate: formic acid (6:5:1, v/v/v) was used as the mobile phase [33]. The derivatisation reagent was prepared by dissolving 40 mg DPPH* in 10 mL of 50% methanol and 50% ethanol and stored in an amber glass bottle, protected from light, until use [34].…”

Section: Sample Preparation 221 Standard Solution and Reagent Prepmentioning

confidence: 99%

Antioxidant HPTLC-DPPH Fingerprinting of Honeys and Tracking of Antioxidant Constituents upon Thermal Exposure

Islam

Sostaric

Lim

et al. 2021

Foods

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The use of High-Performance Thin-Layer Chromatography (HPTLC) coupled with the use of DPPH* (2,2-diphenyl-1-picrylhydrazyl) as a derivatisation reagent is a novel approach to the analysis of antioxidant activity of honeys. The method facilitates the visualisation of individual constituents that contribute to the overall antioxidant activity of the honey, even if they are not yet chemically identified, and allows for the quantification of their antioxidant activity as gallic acid equivalents. The method supports a more in-depth study of the antioxidant activity of honey as it allows for a comparative analysis of the antioxidant fingerprints of honeys of different floral origin and is able to capture differences in their individual bioactive constituents. Further, it supports the tracking of changes in antioxidant activity of individual honey constituents over time upon exposure to different temperature conditions, which demonstrates the potential value of the method for in-process quality control.

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Section: Sample Preparation 221 Standard Solution and Reagent Prepmentioning

confidence: 99%

Antioxidant HPTLC-DPPH Fingerprinting of Honeys and Tracking of Antioxidant Constituents upon Thermal Exposure

Islam

Sostaric

Lim

et al. 2021

Foods

Self Cite

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“…Although HPLC still remains the most dominant technique for the separation and identification of phenolic compounds (Badjah Hadj Ahmed et al 2016, Pascual-Maté et al 2017, HPTLC seems to be a very promising alternative for the analysis of these compounds in honey (Locher et al 2017). This is because HPTLC is simpler, more flexible, more accessible, and cheaper than other commonly used methods.…”

Section: Introductionmentioning

confidence: 99%

“…It is noteworthy that only one paper has been devoted to the analysis of the chemical composition of honey by means of HPTLC. Locher et al 2017 explored HPTLC fingerprinting for the authentication of honey, such as jarrah (Eucalyptus marginata), marri (Corymbia calophyla), and manuka (Leptospermum scoparium), but they did not study the use of the HPTLC method for the determination of phenolic compounds.…”

Section: Introductionmentioning

confidence: 99%

HPTLC Phenolic Profiles as Useful Tools for the Authentication of Honey

Stanek

Jasicka-Misiak

2018

Food Anal. Methods

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The present study reveals the utility of high-performance thin-layer chromatographic (HPTLC) fingerprinting of phenolic constituents for the authentication of monofloral honeys. The obtained data enables a more complete assessment of honey quality and the identification of emerging threats to honey quality. The developed procedure facilitates differentiation of varietal honeys and detection of honey adulterations. We used an HPTLC fingerprint analysis to determine the characteristic patterns of different honey types (willow, buckwheat, heather, pine honeydew, and manuka honey). The HPTLC chromatograms were used to determine the differences in the botanical origin of the honey samples on the basis of the band profiles, which are characteristic for each honey type. Identification of 11 polyphenols was performed by comparison of the color and R f of the bands with available standards. Additionally, the results were confirmed by an HPLC analysis.

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“…Spectroscopic methods such as NMR, FT-IR and NIR have also been successfully used for metabolomic studies [ 16 , 24 , 25 , 26 ]. There are limited reports about the usage of HPTLC [ 27 , 28 ], capillary electrophoresis [ 29 ], amperometric methods [ 30 ], ion chromatography [ 31 ] and even immunoassays [ 32 ]. Of course, the obtained results (including profiles and metabolomes) are strongly dependent on the isolation technique used to obtain the honey fraction and on the methods used to determine its composition.…”

Section: Introductionmentioning

confidence: 99%

“…To the best of our knowledge, this work is one of the first reports on the use of HPTLC for the determination of the botanical origin of honey samples. So far, HPTLC procedures have been successfully applied in the differentiation of manuka and kanuka honeys [ 28 ] and for differentiation honeys based on the identified phenolic compounds [ 33 ]. Both of these works indicate the great potential of the usage of HPTLC for fast and accurate differentiation of honeys based on their botanical origin.…”

Section: Introductionmentioning

confidence: 99%

HPTLC Fingerprinting—Rapid Method for the Differentiation of Honeys of Different Botanical Origin Based on the Composition of the Lipophilic Fractions

et al. 2018

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Bee honey possess various nutritional and medicinal functions, which are the result of its diverse chemical composition. The numerous bioactive compounds in honey come from flower nectar; thus, the identification of the specific chemical profiles of honey samples is of great importance. The lipophilic compounds from eight monofloral honeys (rape, buckwheat, clover, willow, milk thistle, dandelion, raspberry and sweet yellow clover) were investigated. Analyses of the lipophilic fractions obtained by UAE (ultrasound assisted extraction) and SPE (solid phase extraction) extractions were performed using high-performance thin layer chromatography (HPTLC). Chromatographic and cluster analyses allowed the identification of a unique, colorful pattern of separated compounds with specific Rf values on the HPTLC plate for each type of monofloral honey. HPTLC is a simple and effective visual method of analysis, and it can serve as a basis for authenticating different types of honey.

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Authentication of honeys of different floral origins via high-performance thin-layer chromatographic fingerprinting

Cited by 35 publications

References 14 publications

Antioxidant HPTLC-DPPH Fingerprinting of Honeys and Tracking of Antioxidant Constituents upon Thermal Exposure

Antioxidant HPTLC-DPPH Fingerprinting of Honeys and Tracking of Antioxidant Constituents upon Thermal Exposure

HPTLC Phenolic Profiles as Useful Tools for the Authentication of Honey

HPTLC Fingerprinting—Rapid Method for the Differentiation of Honeys of Different Botanical Origin Based on the Composition of the Lipophilic Fractions

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