Authentication of camel meat using species-specific PCR and PCR-RFLP

Vaithiyanathan, S.; Vishnuraj, M. R.; Reddy, Godumagadda Narender; Srinivas, C. V.

doi:10.1007/s13197-020-04849-w

Cited by 15 publications

(7 citation statements)

References 24 publications

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“…This method can be applied as a tool for improving mislabeling issues, traceability, and authentication. Determination of beef‐jerky species variation, authentication of camel meat and shrimp species are some recent applications of PCR‐RFLP method 44–46 . In the present study, sequencing analysis of coi gene of fish eggs, and digestion with selected restriction enzymes was performed and proved to be efficient for authentication purposes.…”

Section: Discussionmentioning

confidence: 86%

“…Determination of beef-jerky species variation, authentication of camel meat and shrimp species are some recent applications of PCR-RFLP method. [44][45][46] In the present study, sequencing analysis of coi gene of fish eggs, and digestion with selected restriction enzymes was performed and proved to be efficient for authentication purposes. From the above results, it is clear that Greek PDO "avgotaracho Mesolonghiou" can be fully discriminated from other samples by restriction enzymes and PCR-RFLP protocol.…”

Section: Pcr-rflp For Coi Genementioning

confidence: 96%

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Potential biological markers by DNA‐based tools for determination of Greek PDO geographical origin and authenticity: “Avgotaracho Mesolonghiou” and “Vostizza currant”

et al. 2021

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Background: Food traceability and authentication had become mandatory for food industry and global food trade. Numerous DNA-based methods could contribute against food frauds, because of their advantages such as simplicity, accuracy, and robustness.The aim of this study was to explore whether unique biological markers for two high valuable and popular Greek protected designation of origin (PDO) products could be indicated. For this purpose, "Avgotaracho Mesolonghiou" known as Greek caviar and "Vostizza" currant were subjected to DNA-based analysis. PCR-RAPD, PCR-RFLP, and PCR-DGGE were performed for Greek PDO products and potential biological markers were explored, based on either genomic DNA or bacteria communities.Results: Band profiles resulted in molecular techniques, could be used as a "barcode" to certify the origin and authenticity of PDO products. Conclusion:These methods are proposed, as alternative traceability tools, in order to provide unique markers and could be the key to "farm to table" challenge. Therefore, biological markers could be used throughout the entire commercial supply chain and protect food products, which hold a quality scheme, from adulterations.

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Section: Discussionmentioning

confidence: 86%

Section: Pcr-rflp For Coi Genementioning

confidence: 96%

Potential biological markers by DNA‐based tools for determination of Greek PDO geographical origin and authenticity: “Avgotaracho Mesolonghiou” and “Vostizza currant”

et al. 2021

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“…Sample DNA templates and primers are critical components of any PCR analysis, as they are the primary determinants of its specificity, sensitivity, and robustness [31]. Therefore, the purity of milk powder samples and the specificity of primers have a great influence on the results of subsequent experiments, which are related to the success or failure of the experiments [32]. The PCR amplification results for camel, ovine, and bovine milk powder DNA showed that only the expected products amplified by specific primers for the corresponding sample showed positive amplification, and the remaining samples did not produce nontarget bands (Figure 2).…”

Section: Results Of Qualitative Detection 321 Validation Of Primersmentioning

confidence: 99%

Detection of Ovine or Bovine Milk Components in Commercial Camel Milk Powder Using a PCR-Based Method

Hao

et al. 2022

Molecules

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Food ingredient adulteration, especially the adulteration of milk and dairy products, is one of the important issues of food safety. The large price difference between camel milk powder, ovine, and bovine milk powder may be an incentive for the incorporation of ovine and bovine derived foods in camel milk products. This study evaluated the use of ordinary PCR and real-time PCR for the detection of camel milk powder adulteration based on the presence of ovine and bovine milk components. DNA was extracted from camel, ovine, and bovine milk powder using a deep-processed product column DNA extraction kit. The quality of the extracted DNA was detected by amplifying the target sequence from the mitochondrial Cytb gene, and the extracted DNA was used for the identification of milk powder based on PCR analysis. In addition, PCR-based methods (both ordinary PCR and real-time PCR) were used to detect laboratory adulteration models of milk powder using primers targeting mitochondrial genes. The results show that the ordinary PCR method had better sensitivity and could qualitatively detect ovine and bovine milk components in the range of 1% to 100% in camel milk powder. The commercial camel milk powder was used to verify the practicability of this method. The real-time PCR normalization system has a good exponential correlation (R2 = 0.9822 and 0.9923) between ovine or bovine content and Ct ratio (specific/internal reference gene) and allows for the quantitative determination of ovine or bovine milk contents in adulterated camel milk powder samples. Accuracy was effectively validated using simulated adulterated samples, with recoveries ranging from 80% to 110% with a coefficient of variation of less than 7%, exhibiting sufficient parameters of trueness. The ordinary PCR qualitative detection and real-time PCR quantitative detection method established in this study proved to be a specific, sensitive, and effective technology, which is expected to be used for market detection.

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“…Within the mitochondrial genes, 16S rRNA has been used for broad range of mammalian and birds' species because of its evolutionary stability (Vences et al, 2016;Ha et al, 2017;Lalitha & Chandavar, 2017). Furthermore, the developed direct PCR method is coupled with RFLP assay which is much more desirable for molecular based meat identification, especially, in cases where large number of samples have to be processed as it does not require DNA sequencing and/or specialized equipment and reduces the cost and time for post-PCR processing of samples (Guan et al, 2019;Gargouri et al, 2021;Vaithiyanathan et al, 2021;Taha et al, 2021). It has been mostly applied for species discrimination in processed and unprocessed meat products because of its simplicity, quicker detection and reduced cost (Al et al, 2020;Asghar et al, 2022;Farag et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Comparison of different DNA preparatory methods for development of a universal direct PCR-RFLP workflow for identification of meat origin in food products

et al. 2023

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The quality and quantity of the extracted DNA are two key aspects for a successful PCR (Polymerase Chain Reaction) amplification. Moreover, reduction in time and cost required for DNA extraction are also two considerable factors, in cases when large number of samples are to be analyzed within a limited time-frame and budget. Accordingly, the aim of this study was to compare and optimize performance of five different DNA extraction methods by boiling meat tissues from cattle, buffalo, sheep, goat, chicken, camel, horse and dog in PBS (Phosphate Buffer Saline), distilled water, alkaline lysis buffers 1, 2 or 3. The results indicated that the boiling of meat and its products in alkaline lysis buffers was a good method to extract crude DNA. The optimized crude DNA extraction protocol was coupled with PCR-RFLP (Restriction Fragment Length Polymorphism) analysis for meat species differentiation. This developed workflow was tested on fifty-three commercial beef and mutton samples, out of which three samples were found to be adulterated. In conclusion, the rapid crude DNA extraction protocol has led to the development of a direct PCR-RFLP workflow that is simple, time-saving and cost-effective for PCR-based identification of different meat species.

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Authentication of camel meat using species-specific PCR and PCR-RFLP

Cited by 15 publications

References 24 publications

Potential biological markers by DNA‐based tools for determination of Greek PDO geographical origin and authenticity: “Avgotaracho Mesolonghiou” and “Vostizza currant”

Potential biological markers by DNA‐based tools for determination of Greek PDO geographical origin and authenticity: “Avgotaracho Mesolonghiou” and “Vostizza currant”

Detection of Ovine or Bovine Milk Components in Commercial Camel Milk Powder Using a PCR-Based Method

Comparison of different DNA preparatory methods for development of a universal direct PCR-RFLP workflow for identification of meat origin in food products

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