Augmentation of CCL17 and CCL28 gene expression by TNF-α, IL-1β, or IFN-γ in cultured canine keratinocytes

Shibata, Sanae; Maeda, Sadatoshi; Maeda, Shingo; Chimura, Naoki; Kondo, N; Fukata, Tsuneo

doi:10.1016/j.rvsc.2009.11.011

Cited by 24 publications

(21 citation statements)

References 26 publications

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“…Although CCL28 levels remain unchanged in these ovarian cell lines, TNF has been shown to increase CCL28 in cultured canine keratinocytes [21] and in A549 human airway epithelial-like cells, via NF-κB signaling [22]. These differences indicate that the chemokine network is likely to be differentially regulated in various cell types.…”

Section: Discussionmentioning

confidence: 99%

Characteristics of chemokine signatures elicited by EGF and TNF in ovarian cancer cells

et al. 2013

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BackgroundOvarian cancer, an inflammation-associated cancer, is the fifth leading cause of cancer deaths in women. The malignancy produces a large amount of tumor necrosis factor (TNF) which promotes a proinflammatory tumor microenvironment. In addition, the epidermal growth factor receptor (EGFR) is frequently overexpressed in high-grade ovarian cancer, which likely aggravates cancer progression. Since ovarian cancer progression is closely associated with chemokine networks driven by inflammation or EGFR activation, we investigated the chemokine signatures elicited by EGF and TNF in ovarian cancer cells to determine their individual profiles and if there was in fact some kind of synergy between their actions on the chemokine network.MethodsWe used a PCR array for the chemokine network to examine the signature of chemokines and their receptors elicited by EGF and TNF in four ovarian cancer cell lines (OVCAR-3, SKOV-3, CaOV-3 and TOV-21G).ResultsThe chemokine network revealed that ovarian cancer cells commonly expressed high levels of proinflammatory chemokines such as CCL20, CXCL1-3 and CXCL8 in response to EGF or TNF. However, the responsiveness to EGF or TNF displayed a cell line specific pattern. Although OVCAR-3 and SKOV-3 cells were responsive to either EGF or TNF, their TNF responsiveness was dominant. On the other hand, CaOV-3 and TOV-21G cells were responsive to EGF but less to TNF, probably due to the high levels of non-canonical nuclear factor (NF)-κB components such as IKKα and p52 in these cell lines compared to OVCAR-3 and SKOV-3 cells. Among chemokine receptors, only CXCR5 was responsive to EGF or TNF in CaOV-3 cells. Finally, CCL20 and CXCL8 responded synergistically in response to EGF and TNF in OVCAR-3 and SKOV-3 cells.ConclusionOur results indicate that CCL20, CXCL1-3 and CXCL8 are the primary chemokines induced by EGF or TNF and are elicited in these ovarian cancer cells via NF-κB, Akt and Erk signaling pathways. Of interest, there was a syngergistic response in terms of CCL20 and CXCL8 levels, when OVCAR-3 and SKOV-3 cells were exposed to EGF plus TNF. Targeting these proinflammatory chemokines may be a promising therapeutic strategy for ovarian cancer with abundant TNF and EGFR activation patterns.

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Section: Discussionmentioning

confidence: 99%

Characteristics of chemokine signatures elicited by EGF and TNF in ovarian cancer cells

et al. 2013

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“…Levels of chemokines and adhesion molecules, like ICAM-1 and VCAM-1 on endothelial cells, are increased and recruited by a variety of leukocytes, such as lymphocytes, mast cells, eosinophils, neutrophils, to skin lesion [33][34][35]. Furthermore, the interaction between CCL17 and CCR4 is regulated by the infiltration of Th2 cells into AD lesions by chemotaxis [36] and in human AD, histological findings have shown that the majority of skin-infiltrating T cells in active AD lesion are CD4 + T cells [37]. We also found that DNFB treatment increased the number of CD4 + T cells infiltrating skin lesions.…”

Section: Discussionmentioning

confidence: 99%

Rosmarinic acid attenuates 2,4-dinitrofluorobenzene-induced atopic dermatitis in NC/Nga mice

Jang

Kim

et al. 2011

International Immunopharmacology

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“…Total RNA was isolated from the cultured primary keratinocytes using the RNeasy Mini Kit (Qiagen; Valencia, CA, USA) according to the manufacturer's manual and the quantification of CCL2 and CCL17 mRNA by real‐time PCR was performed as described previously . Glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) was used as a reference gene . The primers sequences for CCL2, CCL17 and GAPDH were designed as previously described (Supporting Information Table ) .…”

Section: Methodsmentioning

confidence: 99%

Diluted sodium hypochlorite (bleach) in dogs: antiseptic efficacy, local tolerability and in vitro effect on skin barrier function and inflammation

Banović

Olivry

Bäumer

et al. 2017

Veterinary Dermatology

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Augmentation of CCL17 and CCL28 gene expression by TNF-α, IL-1β, or IFN-γ in cultured canine keratinocytes

Cited by 24 publications

References 26 publications

Characteristics of chemokine signatures elicited by EGF and TNF in ovarian cancer cells

Characteristics of chemokine signatures elicited by EGF and TNF in ovarian cancer cells

Rosmarinic acid attenuates 2,4-dinitrofluorobenzene-induced atopic dermatitis in NC/Nga mice

Diluted sodium hypochlorite (bleach) in dogs: antiseptic efficacy, local tolerability and in vitro effect on skin barrier function and inflammation

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