Atypical phenotypic characteristics of klebsiella pneumoniae isolates from an outbreak in a neonatal intensive care unit in Brazil

Otman, Jamile; Perugini, Marcia Regina Eches; Tognim, Maria Cristina Bronharo; Vidotto, Marilda Carlos

doi:10.1590/s1517-83822007000200016

Cited by 11 publications

(9 citation statements)

References 13 publications

(12 reference statements)

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“…This bacterium is one of the leading causes of community-acquired and nosocomial infections and is associated with high mortality rates (2) . The prevalence of K. pneumoniae with multi-drug resistance, which commonly leads to systemic infections and death, has been increasing in recent years (3) . The emergence of infections caused by these multidrug-resistant strains is a clinical challenge, with limited and complex treatment options (4) .…”

Section: Introductionmentioning

confidence: 99%

First report of TEM-104-, SHV-99-, SHV-108-, and SHV-110-producing Klebsiella pneumoniae from Iran

Zahedani

Rigi

Bokaeian

et al. 2016

Rev. Soc. Bras. Med. Trop.

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Introduction: Extended-spectrum beta-lactamases (ESBLs) are bacterial enzymes capable of hydrolyzing beta-lactams. The aim of this study was to describe the prevalence of TEM-and SHV-type ESBL-producing Klebsiella pneumoniae strains in Zahedan, Southeast Iran. Methods: A total of 170 non-repetitive K. pneumoniae strains were collected from patients referred to three teaching hospitals of Zahedan. Antibiotic susceptibility testing was determined for 17 antibiotics using the Kirby-Bauer disc diffusion method. The frequency of ESBL-producing strains was calculated, and minimum inhibitory concentrations of ESBLproducing strains were determined for cefotaxime, ceftazidime, ceftriaxone, and cefpodoxime. The presence of bla TEM and bla SHV genes was tested in all ESBL-producing strains using polymerase chain reaction and DNA sequencing. Results: Among the 170 K. pneumoniae clinical isolates, 55 (32.4%) were ESBL producers; 92.7% (n=51) and 72.7% (n=40) of the isolates carried the bla SHV and bla TEM genes, respectively, and 67.3% (n=37) carried both genes. The sequencing results showed that all bla TEM types were bla TEM-1 , except for two isolates that were bla . The bla SHV types were bla SHV-1 , bla SHV-11 , bla , bla SHV-99 , bla , and bla . Conclusions: The percentage of bla TEM and bla SHV among ESBL-producing K. pneumoniae isolates from Zahedan is relatively high, indicating the need for further surveillance and consideration in antibiotic use. To the best of our knowledge, this is the fi rst report of TEM-104-, SHV-99-, SHV-108-, and SHV-110-type ESBLs among clinical isolates of K. pneumoniae from Iran, and TEM-1, SHV-1, SHV-11, and SHV-12 appear to be the dominant ESBLs in this region.

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Section: Introductionmentioning

confidence: 99%

First report of TEM-104-, SHV-99-, SHV-108-, and SHV-110-producing Klebsiella pneumoniae from Iran

Zahedani

Rigi

Bokaeian

et al. 2016

Rev. Soc. Bras. Med. Trop.

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“…La capacidad de K. pneumoniae de propagarse con rapidez entre pacientes de algunos servicios hospitalarios a menudo conduce a brotes de infección intrahospitalaria, especialmente en los recién nacidos (7,8). Los métodos de genotipificación de los aislamientos como PFGE son recomendados en el estudio de estos casos; así como en la vigilancia epidemiológica.…”

Section: Discussionunclassified

“…Es una bacteria Gram negativa perteneciente a la familia Enterobacteriaceae que posee mecanismos de resistencia antimicrobiana a los antibióticos β-lactámicos (3) y que causa gran morbilidad y mortalidad, especialmente en unidades de cuidados intensivos, neonatales, médicos y quirúrgicos (6)(7)(8)(9)(10). En América Latina, es el tercer patógeno más frecuente aislado en el tracto respiratorio de los pacientes hospitalizados con neumonía y corresponde al 12% de todos los patógenos aislados (11).…”

Section: Introductionunclassified

“…Entre las técnicas disponibles para K. pneumoniae se incluyen la electroforesis en gel de campo pulsado (PFGE) (17,18), tipificación por secuencias de locus múltiples (MLST) (1,2,4), el perfil plasmídico (19), la ribotipificación (20) y métodos basados en la reacción en cadena de la polimerasa (PCR), como la detección de elementos extragénicos repetitivos palindrómicos (REP) (17,21,22) y consenso de elementos repetitivos intergénicos de enterobacterias (ERIC) (8,(20)(21)(22)(23)(24). En las últimas décadas, K pneumoniae portadora de BLEE ha sido responsable de brotes epidémicos en países de todo el mundo (5,25).…”

Section: Introductionunclassified

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Caracterización molecular de cepas de Klebsiella pneumoniae productoras de BLEE causantes de infección intrahospitalaria en el servicio de neonatología de un hospital de Lima, Perú

Bailón

Sacsaquispe

2013

Rev Med Hered

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“…Some authors have reported major single-strain outbreaks (12,24), whereas others emphasized the importance of plasmid transfer rather than the strain spread (3). The current study demonstrates that the R plasmids transferring and spreading the multidrug-resistance between different enterobacterial genera and species isolated from both hospitalized patients and outpatients in a 600-bed university hospital.…”

Section: Introductionmentioning

confidence: 99%

Horizontol dissemination of TEM- and SHV-typr beta-lactamase genes-carrying resistance plasmids amongst clonical isolates of Enterobacteriaceae

Özgümüş¹,

Tosun²,

Aydın³

et al. 2008

Braz. J. Microbiol.

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The extended-spectrum β-lactamase (ESBL)-producing bacteria have been isolated at increasing frequency worldwide. Expression of ESBL is often associated with multidrug resistance and dissemination by resistance plasmids. During a two-month period in 2000, 133 clinical isolates of enterobacterial strains were randomly collected from outpatients and inpatients at a university hospital in Turkey. The ESBL producing strains were determined by double-disk synergy (DDS) testing. Twenty ESBL producing strains (15%) including Escherichia coli (n = 9), Klebsiella pneumoniae (n = 7), Klebsiella oxytoca (n = 2) and Enterobacter aerogenes (n = 2) were detected and further analyzed for their resistance transfer features, plasmid profile and nature of the resistance genes. Plasmid transfer assays were performed using broth mating techniques. TEM- and SHV- genes were analyzed by polymerase chain reaction (PCR) and hybridization using specific probes. EcoRI restriction enzyme analyses of R plasmids were used in the detection of epidemic plasmids. Fourteen plasmid profiles (A, B1, B2, C1, and C2 to L) were obtained with EcoRI restriction enzyme analysis. Most of these plasmids were detected to carry both TEM- and SHV-derived genes by PCR, and confirmed by localizing each gene by hybridization assay. Epidemiological evidence indicated that there was an apparent horizontal dissemination of conjugative R plasmids among multidrug-resistant enterobacterial genera and species in this hospital.

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Atypical phenotypic characteristics of klebsiella pneumoniae isolates from an outbreak in a neonatal intensive care unit in Brazil

Cited by 11 publications

References 13 publications

First report of TEM-104-, SHV-99-, SHV-108-, and SHV-110-producing Klebsiella pneumoniae from Iran

First report of TEM-104-, SHV-99-, SHV-108-, and SHV-110-producing Klebsiella pneumoniae from Iran

Caracterización molecular de cepas de Klebsiella pneumoniae productoras de BLEE causantes de infección intrahospitalaria en el servicio de neonatología de un hospital de Lima, Perú

Horizontol dissemination of TEM- and SHV-typr beta-lactamase genes-carrying resistance plasmids amongst clonical isolates of Enterobacteriaceae

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