Attaching and effacing activities of rabbit and human enteropathogenic Escherichia coli in pig and rabbit intestines

Moon, Harley W.; Whipp, S C; Argenzio, RA; Levine, Myron M.; Giannella, Ralph A.

doi:10.1128/iai.41.3.1340-1351.1983

Cited by 796 publications

(451 citation statements)

References 17 publications

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“…JPN 15 (pMAR7) EPEC strain we observed that the localized circumscribed clusters characteristic of EPEC adherence [32,43,541 occured only in differentiated enterocyte-like HT-29 glc-/+. Moreover, we observed that the cluster-localized binding of EPEC bacteria to Caco-2 cells, which spontaneously differentiate in culture [5], developed in parallel with cell differentiation, disclosed by immunofluorescence labeling of sucrase-isomaltase, a differentiation-associated brush border hydrolase [55].…”

Section: Work Presented In This Communication Analyses the Cellassocimentioning

confidence: 75%

Two stages of enteropathogenic Escherichia coli intestinal pathogenicity are up and down-regulated by the epithelial cell differentiation

et al. 1995

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Pathogens and eucaryotic cells are active partners during the process of pathogenicity. To gain access to enterocytes and to cross the epithelial membrane, many enterovirulent microorganisms interact with the brush border membrane-associated components as receptors. Recent reports provide evidence that intestinal cell differentiation plays a role in microbial pathogenesis. Human enteropathogenic Escherichia coli (EPEC) develop their pathogenicity upon infecting enterocytes. To determine if intestinal epithelial cell differentiation influences EPEC pathogenicity, we examined the infection of human intestinal epithelial cells by JPN 15 (pMAR7) [EAF+ eae+] EPEC strain as a function of the cell differentiation. The human embryonic intestinal INT407 cells, the human colonic T84 cells, the human undifferentiated HT-29 cells (HT-29 Std) and two enterocytic cell lines, HT-29 glc-/+ and Caco-2 cells, were used as cellular models. Cells were infected apically with the EPEC strain and the cell-association and cell-entry were examined by quantitative determination using metabolically radiolabeled bacteria, as well as by light, scanning and transmission electron microscopy. [EAF+ eae+] EPEC bacteria efficiently colonized the cultured human intestinal cells. Diffuse bacterial adhesion occurred to undifferentiated HT-29 Std and INT407 cells, whereas characteristic EPEC cell clusters were observed on fully differentiated enterocytic HT-29 glc-/+ cells and on colonic crypt T84 cells. As shown using the Caco-2 cell line, which spontaneously differentiates in culture, the formation of EPEC clusters increased as a function of the epithelial cell differentiation. In contrast, efficient cell-entry of [EAF+ eae+] EPEC bacteria occurred in recently differentiated Caco-2 cells and decreased when the cells were fully differentiated.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Work Presented In This Communication Analyses the Cellassocimentioning

confidence: 75%

Two stages of enteropathogenic Escherichia coli intestinal pathogenicity are up and down-regulated by the epithelial cell differentiation

et al. 1995

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“…Diarrhea represents an increasing and recurrent problem in young calves, from 1 week up to 12 weeks of age, especially in suckling beef calves, in farms where the neonatal diarrhea problems have regressed thanks mainly to vaccination and higher hygiene standards. Among the possible infectious agents, the`attaching and effacing' strains of E. coli (AEEC; Moon et al, 1983) have been associated with diarrhea and dysentery in calves up to 2 months of age (Butler and Clarke, 1994;Mainil et al, 1993). However no large scale epidemiological study has been carried out to verify this association mainly because of the difficulty in the diagnosis of the AEEC.…”

Section: Discussionmentioning

confidence: 99%

“…More recently, attaching and effacing E. coli (AEEC) (Moon et al, 1983) have been implicated in diarrhea and dysentery mostly in 2 to 8-week old calves (Chanter et al, 1984;Mainil et al, 1993).``Attaching and effacing'' was the term first used by Moon et al (1983) to describe an intestinal lesion produced by E. coli:`attaching' indicates the intimate attachment of bacteria to the enterocyte;`effacing' signifies the localized effacement of brush border microvilli. Such a lesion appears when enteropathogenic E. coli (EPEC) or enterohaemorrhagic E. coli (EHEC) infect their host.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and molecular typing of attaching and effacing Escherichia coli among calf populations in Belgium

China

Pirson

Mainil

1998

Veterinary Microbiology

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Attaching and effacing Escherichia coli are involved in diarrhea in 2 to 8-week old calves. The virulence factors of these bacteria include: (i) the secretion of proteins (i.e. EspB) involved in microvilli effacement, (ii) the production of the intimin, a 94 kDa outer membrane protein encoded by the eaeA gene and involved in the intimate attachment of bacteria to epithelial cell and (iii) the production of verotoxins: VT1 and/or VT2. We investigated the presence and the pathotype of these strains in several calf populations by colony hybridization or by genetic amplification. Using the colony hybridization method we showed first that only 5% of calves who died from diarrhea presented EaeA+ E. coli strains and secondly that 19% of healthy calves showed an asymptomatic carriage. However, using colony hybridization and genetic amplification, we identified EaeA+ strains in 91% of calves living in farms with recurrent diarrhea problems. In 66% of the calves, there was a correlation between the presence of AEEC and diarrhea. At the pathotype level, most of the EaeA+ isolates were negative for VT probes. In VT+ bacteria, the majority were VT1+. The number of VT positive bacteria was significantly higher in calves who died from diarrhea than in healthy or sick calves. This underlined the aggravating role of verotoxins in the disease. Moreover, only 25% of the bovine AEEC were positive with the EaeB probe. Surprisingly, the proportion of EaeB+ strains was significantly higher in healthy calves than in other populations.

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“…C. parvum and coronavirus were identified in one additional calf. Histopathologic lesions consistent with the attaching and effacing phenotype (Moon et al, 1983;Hall et al, 1985Hall et al, , 1990Chanter et al, 1984Chanter et al, , 1986Moxley and Francis, 1986;Pospischil et al, 1987;Janke et al, 1990) were detected in 10 calves. The lesion was detected in the colon only of six calves and in the ileum and colon of four calves.…”

Section: Postmortem Examination Data and Concurrent Infectionsmentioning

confidence: 96%

“…Calf strains of Escherichia coli possessing the eae gene and having the capacity to induce the attaching and effacing lesion are designated as attaching and effacing E. coli (AEEC) (Moon et al, 1983). Intimin, a 94 kDa outer membrane protein encoded by the eae gene, mediates adherence of AEEC to intestinal epithelial cells; an event critical for the formation of the attaching and effacing lesion (Jerse and Kaper, 1991;Donnenberg and Kaper, 1992).…”

Section: Introductionmentioning

confidence: 99%

Characterization of eae+ Escherichia coli isolated from healthy and diarrheic calves

Holland

Wilson²,

Holland

et al. 1999

Veterinary Microbiology

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Strains of Escherichia coli from 101 healthy and 114 diarrheic calves were screened by PCR for the eae (intimin) gene and Shiga toxin genes (stx). Each eae+ and eae/stx+ strain was examined for antimicrobial susceptibility, enterohemolysin activity, and the somatic O antigen was determined. An immunoassay was used to detect Shiga toxin antigens for the eae/stx+ E. coli. Significantly more (p = 0.005) of the healthy calves carried eae+ and eae/stx+ E. coli in their feces when compared to strains from diarrheic calves. Moreover, Shiga toxin antigens were detected significantly more (p = 0.001) often among the eae/stx+ strains from healthy calves when compared to eae/stx+ strains from diarrheic calves. However, significantly more (p = 0.001) of the eae+ and eae/stx+ strains from diarrheic calves were resistant to at least one of the antimicrobials tested, and the strains from diarrheic calves had a significantly (p = 0.05) higher rate of antimicrobial resistance to at least two different antimicrobial classes. No significant difference (p> or =0.05) was detected among the eae+ and eae/stx+ strains from healthy and diarrheic calves for enterohemolysin production. Serogroups O-negative, O5, O26, and O111 were predominate among both healthy and diarrheic calves.

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Attaching and effacing activities of rabbit and human enteropathogenic Escherichia coli in pig and rabbit intestines

Cited by 796 publications

References 17 publications

Two stages of enteropathogenic Escherichia coli intestinal pathogenicity are up and down-regulated by the epithelial cell differentiation

Two stages of enteropathogenic Escherichia coli intestinal pathogenicity are up and down-regulated by the epithelial cell differentiation

Prevalence and molecular typing of attaching and effacing Escherichia coli among calf populations in Belgium

Characterization of eae+ Escherichia coli isolated from healthy and diarrheic calves

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