2013
DOI: 10.1016/j.fob.2013.01.009
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Abstract: In mouse parotid glands, we found expression of skeletal muscle actin (actin-α1) protein and mRNA. We isolated myoepithelial cells from the mouse parotid glands and investigated their actin-α1 expression because smooth muscle actin (actin-α2) has been used as a marker for myoepithelial cells. We used actin-α1 expression to identify pathological changes in diabetic non-obese diabetic (NOD; NOD/ShiJcl) mice—a mouse model for Sjögren's syndrome—and found myoepithelial cells to be decreased or atrophied in the dia… Show more

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Cited by 15 publications
(14 citation statements)
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“…We also found a novel increase in the myoepithelial SM a-actin staining in the NOD/ShiLtJ SMGs as compared with controls. In the human samples, consistently decreased SM a-actin was present in the SS tissues as well as in non-SS controls as compared with autopsy samples, consistent with previous reports in the NOD/ShiLtJ parotid gland that indicate decreased myoepithelial populations in SS (Nashida et al, 2013). Again, this may reflect that 22 weeks in the NOD/ShiLtJ model is not representative of human late-stage disease, or may indicate glandspecific differences in disease progression since the human biopsies we queried are minor labial glands rather than submandibular glands.…”
Section: Discussionsupporting
confidence: 90%
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“…We also found a novel increase in the myoepithelial SM a-actin staining in the NOD/ShiLtJ SMGs as compared with controls. In the human samples, consistently decreased SM a-actin was present in the SS tissues as well as in non-SS controls as compared with autopsy samples, consistent with previous reports in the NOD/ShiLtJ parotid gland that indicate decreased myoepithelial populations in SS (Nashida et al, 2013). Again, this may reflect that 22 weeks in the NOD/ShiLtJ model is not representative of human late-stage disease, or may indicate glandspecific differences in disease progression since the human biopsies we queried are minor labial glands rather than submandibular glands.…”
Section: Discussionsupporting
confidence: 90%
“…Previous studies have indicated that there is an increase in the ductal cell population accompanying a loss of acinar cells in SS (Daniels, ), although subsequent studies have revealed considerable heterogeneity in the extent of acinar loss and ductal dysplasia in these patients (Dawson et al, ; Beroukas et al, ; Goicovich et al, ). Myoepithelial cells surrounding the acini and some of the ducts promote salivary gland structure by producing basement membrane and regulatory growth factors, and facilitating the secretion of saliva by contraction (Redman, ; Ianez et al, ; Kandagal et al, ; Tamgadge et al, ), and a decrease in the myoepithelial cell population has been reported in the parotid glands of some SS patients (Nashida et al, ). As patients are typically diagnosed late in disease and display considerable heterogeneity in tissue atrophy, cellular mechanisms contributing to disease progression are not well understood.…”
Section: Introductionmentioning
confidence: 99%
“…Pathological analyses of DM‐NOD mice by haematoxylin–eosin staining indicated the destruction of β ‐cells in the pancreas but no apparent change in the parotid glands (Figure ). This is in contrast to the abnormal immunocytochemical findings of parotid glands observed in DM‐NOD and non‐DM‐NOD mice, indicating destruction of myoepithelial cells surrounding acinar cells (Nashida et al , ). Functional failures in the parotid glands may be different from those in the pancreas in NOD mice.…”
Section: Discussionmentioning
confidence: 68%
“…Similarly, there are some other markers, such as skeletal muscle actin, which has been proven to be specific for the MECs of salivary glands, although we are not aware that it has been used to study human MECs or tumours originating from these cells (Nashida et al. ).…”
Section: Discussionmentioning
confidence: 99%
“…Although other useful markers are currently used, such as podoplanin (Hata et al 2008), S100 (Ogawa, 2003;Ianez et al 2010) and p63 (Ianez et al 2010), they lack specificity. Similarly, there are some other markers, such as skeletal muscle actin, which has been proven to be specific for the MECs of salivary glands, although we are not aware that it has been used to study human MECs or tumours originating from these cells (Nashida et al 2013).…”
Section: Discussionmentioning
confidence: 99%