Atrazine, desethylatrazine (DEA) and desisopropylatrazine (DIA) degradation by <i>Pleurotus ostreatus</i> INCQS 40310

Lopes, Raquel de Oliveira; Pereira, Patrícia Maia; Pereira, António; Fernandes, Keysson Vieira; Carvalho, Julia Finamor; França, Alexandre da Silva de; Valente, Richard H.; Silva, Manuela da; Ferreira-Leitão, Viridiana Santana

doi:10.1080/10242422.2020.1754805

Cited by 29 publications

(18 citation statements)

References 73 publications

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“…Phanerochaete chrysosporium removed 48% of initial atrazine from the medium within 4 days [16]. Lopes et al [4] reported a noticeable performance of Pleurotus ostreatus INCQS 40310 degrading 82% and 56% of initial 10 mg L -1 of atrazine and desethylatrazine, respectively, after 15 days in liquid medium; it was the rst work demonstrating the biodegradation of atrazine metabolites DIA and DEA by fungi. The proteome analysis indicated that, in this case, extracellular hydrolases and peroxidases were overexpressed in the extracellular environment.…”

Section: Discussionmentioning

confidence: 98%

“…Lignocellulosic crop residues, specially sugarcane bagasse, are abundant bulk agents to support fungal growth in this context. [4,15,16]. Thus, a nutrient rich culture medium, allowing substantial biomass development, provides best results in terms of biodegradation [22].…”

Section: Discussionmentioning

confidence: 99%

“…Despite the limitations imposed by the incomplete atrazine biodegradation by basidiomycetes in pure cultures, studies applying puri ed ligninolytic enzymes under controlled conditions, or adopting cocultures involving soil bacteria and deuteromycetes, have been successful in achieving complete dissipation of atrazine and detoxi cation of contaminated substrates [4,18,19]. White-rot fungi bioremediation approaches also present the advantage of being low cost processes, once fungal species are usually not fastidious; being mainly saprophytes, their growth can be easily supported by the organic residues, in laboratory culture conditions or in the eld [20].…”

Section: Introductionmentioning

confidence: 99%

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Biodegradation of atrazine and ligninolytic enzyme production by basidiomycete strains 

Henn

Monteiro

Boscolo

et al. 2020

Preprint

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Background Atrazine is one of the most widespread chlorinated herbicides, leaving large bulks in soils and groundwater. The biodegradation of atrazine by bacteria is well described, but many aspects of the fungal metabolism of this compound remain unclear. Thus, we investigated the toxicity and degradation of atrazine by 13 rainforest basidiomycete strains. Results In liquid medium, Pluteus cubensis SXS320, Gloelophyllum striatum MCA7, and Agaricales MCA17 removed 30, 37, and 38%, respectively, of initial 25 mg L-1 of the herbicide within 20 days. Deficiency of nitrogen drove atrazine degradation by Pluteus cubensis SXS320; this strain removed 30% of atrazine within 20 days in a culture medium with 2.5 mM of N, raising three metabolites; in a medium with 25 mM of N, only 21% of initial atrazine were removed after 40 days, and two metabolites appeared in culture extracts. This is the first report of such different outcomes linked to nitrogen availability during the biodegradation of atrazine by basidiomycetes. The herbicide also induced synthesis and secretion of extracellular laccases by Datronia caperata MCA5, Pycnoporus sanguineus MCA16, and Polyporus tenuiculus MCA11. Laccase levels produced by of P. tenuiculus MCA11 were 13.3-fold superior in the contaminated medium than in control; the possible role of this enzyme on atrazine biodegradation was evaluated, considering the strong induction and the removal of 13.9% of the herbicide in vivo. Although 88% of initial laccase activity remained after 6 h, no evidence of in vitro degradation was observed, even though ABTS was present as mediator. Conclusions This study revealed a high potential for atrazine biodegradation among tropical basidiomycete strains. Further investigations, focusing on less explored ligninolytic enzymes and cell-bound mechanisms, could enlighten key aspects of the atrazine fungal metabolism and the role of the nitrogen in the process.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biodegradation of atrazine and ligninolytic enzyme production by basidiomycete strains 

Henn

Monteiro

Boscolo

et al. 2020

Preprint

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“…Atrazine belongs to the chemical class of chlorinated triazines, and is formed by a heterocyclic aromatic ring alternating carbon and nitrogen atoms, besides chlorine substituent. Like other halogenated aromatic compounds, atrazine and its dealkylated and deaminated metabolites are toxic, carcinogenic and endocrine disruptors [3,4].…”

Section: Introductionmentioning

confidence: 99%

“…The resulting metabolites, such as deethylatrazine (DEA), deisopropylatrazine (DIA), deethyldeisopropylatrazine (DEA-DIA), and hydroxyatrazine (HA) still retain the aromatic ring [16,17]. The degradation of atrazine derivatives DIA and DEA by a fungal strain (Pleurotus ostreatus INCQS 40310) was only recently described [4].…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of atrazine and ligninolytic enzyme production by basidiomycete strains

et al. 2020

View full text Add to dashboard Cite

Background: Atrazine is one of the most widespread chlorinated herbicides, leaving large bulks in soils and groundwater. The biodegradation of atrazine by bacteria is well described, but many aspects of the fungal metabolism of this compound remain unclear. Thus, we investigated the toxicity and degradation of atrazine by 13 rainforest basidiomycete strains. Results: In liquid medium, Pluteus cubensis SXS320, Gloelophyllum striatum MCA7, and Agaricales MCA17 removed 30, 37, and 38%, respectively, of initial 25 mg L − 1 of the herbicide within 20 days. Deficiency of nitrogen drove atrazine degradation by Pluteus cubensis SXS320; this strain removed 30% of atrazine within 20 days in a culture medium with 2.5 mM of N, raising three metabolites; in a medium with 25 mM of N, only 21% of initial atrazine were removed after 40 days, and two metabolites appeared in culture extracts. This is the first report of such different outcomes linked to nitrogen availability during the biodegradation of atrazine by basidiomycetes. The herbicide also induced synthesis and secretion of extracellular laccases by Datronia caperata MCA5, Pycnoporus sanguineus MCA16, and Polyporus tenuiculus MCA11. Laccase levels produced by of P. tenuiculus MCA11 were 13.3fold superior in the contaminated medium than in control; the possible role of this enzyme on atrazine biodegradation was evaluated, considering the strong induction and the removal of 13.9% of the herbicide in vivo. Although 88% of initial laccase activity remained after 6 h, no evidence of in vitro degradation was observed, even though ABTS was present as mediator. Conclusions: This study revealed a high potential for atrazine biodegradation among tropical basidiomycete strains. Further investigations, focusing on less explored ligninolytic enzymes and cell-bound mechanisms, could enlighten key aspects of the atrazine fungal metabolism and the role of the nitrogen in the process.

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Pesticide Bioremediation: OMICs Technologies for Understanding the Processes

Rodríguez

Castrejón-Godínez²,

Sánchez-Salinas³

et al. 2022

Pesticides Bioremediation

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Atrazine, desethylatrazine (DEA) and desisopropylatrazine (DIA) degradation by Pleurotus ostreatus INCQS 40310

Cited by 29 publications

References 73 publications

Biodegradation of atrazine and ligninolytic enzyme production by basidiomycete strains

Biodegradation of atrazine and ligninolytic enzyme production by basidiomycete strains

Biodegradation of atrazine and ligninolytic enzyme production by basidiomycete strains

Pesticide Bioremediation: OMICs Technologies for Understanding the Processes

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Atrazine, desethylatrazine (DEA) and desisopropylatrazine (DIA) degradation by Pleurotus ostreatus INCQS 40310

Cited by 29 publications

References 73 publications

Biodegradation of atrazine and ligninolytic enzyme production by basidiomycete strains&nbsp;

Biodegradation of atrazine and ligninolytic enzyme production by basidiomycete strains&nbsp;

Biodegradation of atrazine and ligninolytic enzyme production by basidiomycete strains

Pesticide Bioremediation: OMICs Technologies for Understanding the Processes

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Biodegradation of atrazine and ligninolytic enzyme production by basidiomycete strains

Biodegradation of atrazine and ligninolytic enzyme production by basidiomycete strains