Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionisation Mass Spectrometry: A Review

Creaser, Colin S.; Ratcliffe, Lucy

doi:10.2174/157341106775197420

Cited by 26 publications

(23 citation statements)

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“…In terms of lipid analysis, one of the main advantages of AP-MALDI over vacuum MALDI is the collisional cooling of ions (i.e., the facile removal of ion internal energy by collisions with a 16 neutral gas) during transfer at atmospheric pressure. This results in less fragmentation than conventional MALDI [93,94]. However, this phenomenon can also lead to undesirable matrix/analyte cluster formation [93].…”

Section: Atmospheric Pressure Maldi (Ap-maldi)mentioning

confidence: 99%

Surface analysis of lipids by mass spectrometry: More than just imaging

Ellis

Brown

Panhuis

et al. 2013

Progress in Lipid Research

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Mass spectrometry is now an indispensable tool for lipid analysis and is arguably the driving force in the renaissance of lipid research. In its various forms, mass spectrometry is uniquely capable of resolving the extensive compositional and structural diversity of lipids in biological systems. Furthermore, it provides the ability to accurately quantify molecular-level changes in lipid populations associated with changes in metabolism and environment; bringing lipid science to the "omics" age. The recent explosion of mass spectrometry-based surface analysis techniques is fuelling further expansion of the lipidomics field. This is evidenced by the numerous papers published on the subject of mass spectrometric imaging of lipids in recent years. While imaging mass spectrometry provides new and exciting possibilities, it is but one of the many opportunities direct surface analysis offers the lipid researcher. In this review we describe the current state-ofthe-art in the direct surface analysis of lipids with a focus on tissue sections, intact cells and thin-layer chromatography substrates. The suitability of these different approaches towards analysis of the major lipid classes along with their current and potential applications in the field of lipid analysis are evaluated. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 2 AbstractMass spectrometry is now an indispensable tool for lipid analysis and is arguably the driving force in the renaissance of lipid research. In its various forms, mass spectrometry is uniquely capable of resolving the extensive compositional and structural diversity of lipids in biological systems. Furthermore, it provides the ability to accurately quantify molecular-level changes in lipid populations associated with changes in metabolism and environment;bringing lipid science to the "omics" age. The recent explosion of mass spectrometry-based surface analysis techniques is fuelling further expansion of the lipidomics field. This is evidenced by the numerous papers published on the subject of mass spectrometric imaging of lipids in recent years. While imaging mass spectrometry provides new and exciting possibilities, it is but one of the many opportunities direct surface analysis offers the lipid researcher. In this review we describe the current state-of-the-art in the direct surface analysis of lipids with a focus on tissue sections, intact cells and thin-layer chromatography substrates.The suitability of these different approaches towards analysis of the major lipid classes along with their current and potential applications in the field of lip...

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Section: Atmospheric Pressure Maldi (Ap-maldi)mentioning

confidence: 99%

Surface analysis of lipids by mass spectrometry: More than just imaging

Ellis

Brown

Panhuis

et al. 2013

Progress in Lipid Research

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“…78 In the last couple of years, the number of publications that describe the use or study of AP-MALDI has increased significantly as the commercial plug-and-play technology 79 has become more widely available. A more detailed discussion of the technique and applications is beyond the limited space of this tutorial article and the interested reader is referred to the reviews 80,81 and some of the broad overview articles 82,83 that appeared on the subject. A schematic of a commercial AP-MALDI source coupled to an LCQ ion trap is shown in Fig.…”

Section: Atmospheric Pressure Matrix-assisted Laser Desorption Ionizamentioning

confidence: 99%

“…The coupling with ion trap mass spectrometers, orthogonal acceleration The mechanism of ion generation in AP-MALDI is believed to be the same as in vacuum MALDI (although that issue is not completely settled) 84 and is believed to be as efficient. 80 One difference between vacuum MALDI and AP-MALDI mass spectra is that the latter generally contain less fragmentation, because of the rapid collisional cooling that occurs at AP. This same process benefits all the LD/I techniques.…”

Section: Atmospheric Pressure Matrix-assisted Laser Desorption Ionizamentioning

confidence: 99%

Established and emerging atmospheric pressure surface sampling/ionization techniques for mass spectrometry

2008

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The number and type of atmospheric pressure techniques suitable for sampling analytes from surfaces, forming ions from these analytes, and subsequently transporting these ions into vacuum for interrogation by MS have rapidly expanded over the last several years. Moreover, the literature in this area is complicated by an explosion in acronyms for these techniques, many of which provide no information relating to the chemical or physical processes involved. In this tutorial article, we sort this vast array of techniques into relatively few categories on the basis of the approaches used for surface sampling and ionization. For each technique, we explain, as best known, many of the underlying principles of operation, describe representative applications, and in some cases, discuss needed research or advancements and attempt to forecast their future analytical utility.

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“…and laser ablation can be used in conjunction with post-ionization by inductively coupled plasma [92-94], chemical ionization [95, 96], photoionization [97], or electrospray ionization [21, 98-100]. AP MALDI with an IR laser and endogenous matrix [19, 20, 101] and femtosecond pulse width laser matrix-free ionization [102] can be considered ambient ionization methods, but AP MALDI [24, 103] is more accurately described as an AP technique rather than an ambient technique due to the requirement for adding a matrix.…”

Section: Ambient and Atmospheric Pressure Laser Imaging Mass Spectmentioning

confidence: 99%

High resolution laser mass spectrometry bioimaging

Murray

Seneviratne

Ghorai

2016

Methods

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MSI (MSI) was introduced more than five decades ago with secondary ion mass spectrometry (SIMS) and a decade later with laser desorption/ionization (LDI) mass spectrometry (MS). Large biomolecule imaging by matrix-assisted laser desorption/ionization (MALDI) was developed in the 1990s and ambient laser MS a decade ago. Although SIMS has been capable of imaging with a moderate mass range at sub-micrometer lateral resolution from its inception, laser MS requires additional effort to achieve a lateral resolution of 10 μm or below which is required to image at the size scale of single mammalian cells. This review covers untargeted large biomolecule MSI using lasers for desorption/ionization or laser desorption and post-ionization. These methods include laser microprobe (LDI) MSI, MALDI MSI, laser ambient and atmospheric pressure MSI, and near-field laser ablation MS. Novel approaches to improving lateral resolution are discussed, including oversampling, beam shaping, transmission geometry, reflective and through-hole objectives, microscope mode, and near-field optics.

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Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionisation Mass Spectrometry: A Review

Cited by 26 publications

References 0 publications

Surface analysis of lipids by mass spectrometry: More than just imaging

Surface analysis of lipids by mass spectrometry: More than just imaging

Established and emerging atmospheric pressure surface sampling/ionization techniques for mass spectrometry

High resolution laser mass spectrometry bioimaging

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