ATG7 deficiency suppresses apoptosis and cell death induced by lysosomal photodamage

Kessel, David; Price, Michael R.; Reiners, John J.

doi:10.4161/auto.20792

Cited by 99 publications

(101 citation statements)

References 47 publications

(44 reference statements)

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“…When photodamage reduces the number of functional lysosomes, autophagosomes accumulate, but there is no cytoprotective effect. Moreover, depletion of ATG5 does not further alter this result, 16 indicating that increased lysosomal pH is sufficient to impair the cytoprotective role of autophagy.…”

mentioning

confidence: 92%

“…15 Experimental data are consistent with lysosomal photodamage initially leading to a loss of the low lysosomal pH needed for the optimal activity of lysosomal proteases. 16 This can result in an accumulation of autophagosomes that are unable to progress further. Meanwhile, the initiation of apoptosis via interactions involving t-BID will be meting out cell death unimpeded by any cytoprotective process.…”

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confidence: 99%

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Autophagic death probed by photodynamic therapy

Kessel

2015

Autophagy

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mentioning

confidence: 92%

mentioning

confidence: 99%

Autophagic death probed by photodynamic therapy

Kessel

2015

Autophagy

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“…Contudo, nas mesmas condições, os mesmos autores observaram uma resposta citoprotetora da autofagia utilizando células L1210 deficientes em ATG7, uma proteína essencial para a autofagia, pois sua depleção resultou na redução da sobrevivência celular. Porém, ATG7 também parece estar envolvida na indução de apoptose (Kessel et al, 2012). É certo que a autofagia representa um mecanismo de sobrevivência frente a distintos estresses celulares, porém se pergunta como a autofagia faria parte também de um mecanismo de morte celular.…”

Section: Morte Celular Induzida Pela Terapia Fotodinâmicaunclassified

“…Kessel et al (2012) propõem que o fotodano lisossomal suprime as funções citoprotetoras da autofagia e implica em estresse adicional devido ao bloqueio do fluxo autofágico. Não se sabe exatamente como a interrupção do fluxo autofágico contribui para a morte celular induzida pela TFD.…”

Section: Morte Celular Induzida Pela Terapia Fotodinâmicaunclassified

“…Foi demonstrado que a morte celular induzida por DMMB fotoativado é acompanhada do acúmulo de vesículas ácidas em células HaCaT, característica indicativa da ocorrência de morte celular relacionada à autofagia (Santos, 2014 fotossensibilizadores que têm localização lisossomal (Reiners Jr et al, 2010;Agostinis et al,2011;Kessel et al, 2012). Nossa hipótese é que o dano lisossomal induzido por DMMB fotoativado mostrado por Santos (2014) bloqueia a autofagia, leva a uma reciclagem ineficiente de biomoléculas pela via autofágica, resultando no acúmulo fatal de intermediários não processados.…”

Section: Autofagia E Morte Celularunclassified

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Efeito da proteína p53 nas respostas celulares à terapia fotodinâmica com 1,9-dimetil azul de metileno

Abrantes¹

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MiR‐490‐3p inhibits autophagy via targeting ATG7 in hepatocellular carcinoma

Yu-xiang

Liu

2018

IUBMB Life

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The miR-490-3p was transfected into HepG2 cells to explore the correlation between miR-490-3p and hepatocellular carcinoma cell proliferation, apoptosis, and autophagy and its downstream target gene ATG7. Then we could possibly provide a mechanism for the treatment of hepatocellular carcinoma. MiR-490-3p was screened out by fold change > 4 and P < 0.01 using gene microarray data. The expression level of miR-490-3p was tested by qRT-PCR and the prognosis analysis was achieved by using TCGA data. The cell proliferation was tested via colony formation assay and CCK-8 after the miR-490-3p mimics were transfected into HepG2 cells; the variations of cell cycle and apoptosis was examined by flow cytometry assay; the number of autophagosome was observed by electron microscopy and the changes of autophagy-relative protein LC-II and LC-I as well as their ratio was tested by western blot. MiR-490-3p is low expressed in hepatocellular carcinoma cell lines and tissues. The results of TCGA showed that miR-490-3p high expression indicated better prognosis. After HepG2 cells were transfected with miR-490-3p mimics, cell viability was increased, cell proliferation was enhanced, cell cycle was blocked in G0/G1 phase, cell apoptosis rate was increased, the number of autophagosomes was reduced, autophagy-associated protein LC-II was decreased, and LC-I was increased and their ratio was decreased. After 3-MA was added, cell proliferation was declined, cell apoptosis rate was increased. Besides, the autophagy was inhibited by knocking down the ATG7, which promoted the cell apoptosis. MiR-490-3p could suppress cell proliferation, retard cell cycle and upgrade cell apoptosis by inhibiting autophagy in HCC cells via targeting ATG7. © 2018 IUBMB Life, 70(6):468-478, 2018.

show abstract

ATG7 deficiency suppresses apoptosis and cell death induced by lysosomal photodamage

Cited by 99 publications

References 47 publications

Autophagic death probed by photodynamic therapy

Autophagic death probed by photodynamic therapy

Efeito da proteína p53 nas respostas celulares à terapia fotodinâmica com 1,9-dimetil azul de metileno

MiR‐490‐3p inhibits autophagy via targeting ATG7 in hepatocellular carcinoma

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