Asymptomatic Leishmania infection in blood donors from a major blood bank in Northeastern Brazil: a cross-sectional study

Silva, Lucas Portela; Montenegro, Sílvia Maria Lucena; Werkauser, Roberto; Sales, Kamila Gaudêncio da Silva; Soares, F; Costa, Vlaudia Maria Assis; Bezerra, Ana Cristina Barreto; Pinto, Maria B. D. A.; Ferreira, Suzany Maria; Neitzke-Abreu, Herintha Coeto; Dantas‐Torres, Filipe; Lima, Manoel Sebastião da Costa

doi:10.1590/s1678-9946202062092

Cited by 8 publications

(3 citation statements)

References 28 publications

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“…[9][10][11][12] There are large numbers of asymptomatic L. infantum infections, with various studies identifying 1-6% of the general population in endemic regions. 7,13,14 VL case fatality rates in Brazil average 7%, the highest in the world, and show an increasing trend. 12 Hence, understanding the factors that lead to severe disease and mortality is a priority for leishmaniasis research.…”

Section: 501951 Doi: Biorxiv Preprintmentioning

confidence: 99%

Parasite genotype is a major predictor of mortality from visceral leishmaniasis

Grace

Carvalho

Lima

et al. 2022

Preprint

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Visceral leishmaniasis (VL) is a potentially fatal disease mainly caused by Leishmania infantum and L donovani. Disease outcomes have been associated with patient genotype, nutrition, age, sex, co-morbidities, and co-infections. Here, we examine the effects of parasite genetic variation on VL disease severity in Brazil. We sequenced the genomes of 109 L infantum isolates from patients in northeast Brazil and retrieved matching patient clinical data, including mortality, sex, HIV co-infection and laboratory data. We identified genetic differences between parasite isolates. To describe associations between the parasite genotypes and clinical outcomes, we applied quantitative genetics methods of heritability and genome-wide association studies (GWAS), treating clinical outcomes as traits that may be influenced by parasite genotype. Multiple aspects of analysis indicate that parasite genotype affects clinical outcomes. We estimate that parasite genotype explains 83% chance of mortality (narrow sense heritability, h2 = 0.83 ± 0.17), and has a significant relationship with patient sex (h2 = 0.60 ± 0.27). Impacts of parasite genotype on other clinical traits are lower (h2 ± 0.34). GWAS analysis identified multiple parasite genetic loci that were significantly associated with clinical outcomes; CNVs that were significantly associated with mortality, creatinine and bacterial co-infection, jaundice and HIV co-infection; and two SNPs/indels that associate with age and jaundice. Parasite genotype is an important factor in VL disease severity in Brazil. Our analysis indicates that specific genetic differences between parasites act as virulence factors, enhancing risks of severe disease and mortality. More detailed understanding of these virulence factors could be exploited for novel therapies.

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Section: 501951 Doi: Biorxiv Preprintmentioning

confidence: 99%

Parasite genotype is a major predictor of mortality from visceral leishmaniasis

Grace

Carvalho

Lima

et al. 2022

Preprint

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“…There are approximately 5,000 VL cases/year in Brazil, 90% of the cases registered in the Americas ( 9 – 12 ). There are large numbers of asymptomatic L. infantum infections, with various studies identifying 1 to 6% of the general population in regions of endemicity ( 7 , 13 , 14 ). VL case fatality rates in Brazil average 7%, the highest in the world, and show an increasing trend ( 12 ).…”

Section: Introductionmentioning

confidence: 99%

Parasite Genotype Is a Major Predictor of Mortality from Visceral Leishmaniasis

Grace

Carvalho

Lima

et al. 2022

mBio

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“…No Brasil, estudo associou a transfusão sanguínea como um fator de risco para a presença de anticorpos anti-leishmania em pacientes politransfundidos e em hemodiálise (Luz et al, 1997). Outros autores demonstraram a presença do DNA do parasita em doadores de sangue assinomáticos, entretanto, não é realizado nenhum tipo de triagem nos hemocentros para essa parasitose (Silva et al, 2020;França et al, 2018).…”

Section: Introductionunclassified

Diagnóstico da doença de Chagas: Avaliação da reação cruzada em pacientes com leishmaniose visceral

Barcelos

Aguiar

Boni

et al. 2021

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No Brasil, existem diversas regiões endêmicas simultaneamente para leishmaniose e doença de Chagas e como os agentes causadores pertencem ambos à mesma família, Trypanosomatidae, eles compartilham diversos antígenos, que no momento do diagnóstico sorológico, podem promover reações cruzadas. Diversos autores avaliaram a reação cruzada Chagas/leishmaniose em cães, entretanto poucos trabalhos fizeram este questionamento em humanos. O objetivo do presente estudo foi identificar a possibilidade de reação cruzada para o diagnóstico da doença de Chagas em pacientes com leishmaniose visceral curada. Para tanto, foram analisadas 30 amostras de soro de pacientes com leishmaniose visceral curada atendidos no Ambulatório de Infectologia do Hospital Regional de Presidente Prudente, São Paulo. As amostras foram avaliadas em duplicata através de três kits sorológicos para o diagnóstico da doença de Chagas: ensaio imunoenzimático (ELISA), imunofluorescência indireta (IFI) e hemaglutinação indireta (HAI). Das 30 amostras analisadas, apenas o soro de um paciente mostrou-se reagente na IFI, entretanto, a duplicata avaliada não foi positiva, assim como os resultados de ELISA e HAI. No presente estudo, devido à reatividade para a IFI apresentar apenas um T. cruzi marcado, com a sua duplicata e outros testes sorológicos (ELISA e HAI) negativos, podemos concluir que o paciente com leishmaniose visceral apresentou uma reação cruzada para o diagnóstico da Doença de Chagas.

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Asymptomatic Leishmania infection in blood donors from a major blood bank in Northeastern Brazil: a cross-sectional study

Abstract: This is an open-access article distributed under the terms of the Creative Commons Attribution License.

Cited by 8 publications

References 28 publications

Parasite genotype is a major predictor of mortality from visceral leishmaniasis

Parasite genotype is a major predictor of mortality from visceral leishmaniasis

Parasite Genotype Is a Major Predictor of Mortality from Visceral Leishmaniasis

Diagnóstico da doença de Chagas: Avaliação da reação cruzada em pacientes com leishmaniose visceral

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