Asymmetric Accumulation of Ash1p in Postanaphase Nuclei Depends on a Myosin and Restricts Yeast Mating-Type Switching to Mother Cells

Bobola, Nicoletta; Jansen, Ralf‐Peter; Shin, Tae Ho; Nasmyth, Kim

doi:10.1016/s0092-8674(00)81048-x

Cited by 325 publications

(312 citation statements)

References 41 publications

(29 reference statements)

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“…Therefore, it might be the defect of actin cable-based transport, which leads to the phenotype observed when AgBNI1 is deleted. It is known from several studies, that actin cables can serve as tracks for different cargos such as secretory vesicles (Johnston et al, 1991) and several organelles (Simon et al, 1995;Hill et al, 1996;Hoepfner et al, 2001;Rossanese et al, 2001) as well as mRNA (Bobola et al, 1996;Sil and Herskowitz, 1996;Takizawa et al, 2000). Using the AgSec4p fused to GFP, we were able to show that secretory vesicles use actin cables for accumulation at the hyphal tips.…”

Section: Agbni1p and The Actin Cytoskeletonmentioning

confidence: 86%

From Function to Shape: A Novel Role of a Formin in Morphogenesis of the FungusAshbya gossypii

Schmitz

Kaufmann

Köhli

et al. 2006

MBoC

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Morphogenesis of filamentous ascomycetes includes continuously elongating hyphae, frequently emerging lateral branches, and, under certain circumstances, symmetrically dividing hyphal tips. We identified the formin AgBni1p of the model fungus Ashbya gossypii as an essential factor in these processes. AgBni1p is an essential protein apparently lacking functional overlaps with the two additional A. gossypii formins that are nonessential. Agbni1 null mutants fail to develop hyphae and instead expand to potato-shaped giant cells, which lack actin cables and thus tip-directed transport of secretory vesicles. Consistent with the essential role in hyphal development, AgBni1p locates to tips, but not to septa. The presence of a diaphanous autoregulatory domain (DAD) indicates that the activation of AgBni1p depends on Rho-type GTPases. Deletion of this domain, which should render AgBni1p constitutively active, completely changes the branching pattern of young hyphae. New axes of polarity are no longer established subapically (lateral branching) but by symmetric divisions of hyphal tips (tip splitting). In wild-type hyphae, tip splitting is induced much later and only at much higher elongation speed. When GTP-locked Rho-type GTPases were tested, only the young hyphae with mutated AgCdc42p split at their tips, similar to the DAD deletion mutant. Two-hybrid experiments confirmed that AgBni1p interacts with GTP-bound AgCdc42p. These data suggest a pathway for transforming one axis into two new axes of polar growth, in which an increased activation of AgBni1p by a pulse of activated AgCdc42p stimulates additional actin cable formation and tip-directed vesicle transport, thus enlarging and ultimately splitting the polarity site.

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Section: Agbni1p and The Actin Cytoskeletonmentioning

confidence: 86%

From Function to Shape: A Novel Role of a Formin in Morphogenesis of the FungusAshbya gossypii

Schmitz

Kaufmann

Köhli

et al. 2006

MBoC

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“…Other Swi5 target genes control events later in G 1 phase. The ASH1 and HO genes are both involved in regulating the initiation of mating type switching; the timing of the mating type switching event is precisely controlled and restricted to late G 1 phase before the initiation of DNA replication (Amon, 1996;Babola et al, 1996). The CDC6 and SIC1 genes are Swi5 targets involved in the proper timing and initiation of DNA replication (Piatti et al, 1995;Knapp et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

A role for the Pcl9‐Pho85 cyclin–cdk complex at the M/G₁ boundary in Saccharomyces cerevisiae

Tennyson¹,

Lee²,

Andrews³

1998

Molecular Microbiology

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SummaryPHO85 is a cyclin-dependent kinase (CDK) with roles in phosphate and glycogen metabolism and cell cycle progression. As a CDK, Pho85 is activated by association with Pho85 cyclins (Pcls), of which 10 are known. PCL1, PCL2 and PCL9 are the only members of the Pho85 cyclin family that are expressed in a cell cycleregulated pattern. We found that PCL9 is expressed in late M/early G 1 phase of the cell cycle and is activated by the transcription factor, Swi5. This pattern of regulation is different from PCL1 and PCL2, which are expressed later in G 1 phase and are regulated primarily by the transcription factor SBF. Co-immunoprecipitation experiments using in vitro translated proteins showed that Pcl9 and Pho85 form a complex. Furthermore, immunoprecipitated Pcl9 complexes from yeast lysates were capable of phosphor ylating the exogenous substrate Pho4. The Pcl9-associated kinase activity was dependent on PHO85, showing that Pcl9 and Pho85 form a functionally active kinase complex in vivo. Deletion of PCL9 in diploid cells caused random, rather than bipolar, budding in 18% of cells. In contrast, deletion of PCL2, the closest relative of PCL9, had no effect on the budding pattern. Deleting more members of the PCL1,2 subfamily (which includes PCL9 ) increased the percentage of random budding in the cell population. When all members of the PCL1,2 subfamily were deleted, 73% of cells budded randomly, a value similar to that obtained when the CDK partner PHO85 was deleted. Our results show that PCL9 and PHO85 form a functional kinase complex and suggest a role for Pho85 CDKs at the M/G 1 boundary.

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“…GAL-CLN3 may repress some of these genes, whereas GAL-CLB2 has no consistent effect on the expression of these genes. Several of these genes are known to be regulated by the transcription factor Swi5p, which itself is a member of the CLB2 cluster (Dohrmann et al, 1992;Bobola et al, 1996;Knapp et al, 1996). Swi5p is thought to bind to a site with the consensus ACCAGC (Knapp et al, 1996), and indeed, when we searched for common motifs in the 5Ј regions of the SIC1 cluster, we found the consensus RRCCAGCR in many of the 27 genes.…”

Section: The M/g1 Clustersmentioning

confidence: 97%

Comprehensive Identification of Cell Cycle–regulated Genes of the YeastSaccharomyces cerevisiaeby Microarray Hybridization

Spellman

Sherlock

Zhang

et al. 1998

MBoC

4,305

118

4,411

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We sought to create a comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle. To this end, we used DNA microarrays and samples from yeast cultures synchronized by three independent methods: ␣ factor arrest, elutriation, and arrest of a cdc15 temperature-sensitive mutant. Using periodicity and correlation algorithms, we identified 800 genes that meet an objective minimum criterion for cell cycle regulation. In separate experiments, designed to examine the effects of inducing either the G1 cyclin Cln3p or the B-type cyclin Clb2p, we found that the mRNA levels of more than half of these 800 genes respond to one or both of these cyclins. Furthermore, we analyzed our set of cell cycle-regulated genes for known and new promoter elements and show that several known elements (or variations thereof) contain information predictive of cell cycle regulation. A full description and complete data sets are available at

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Asymmetric Accumulation of Ash1p in Postanaphase Nuclei Depends on a Myosin and Restricts Yeast Mating-Type Switching to Mother Cells

Cited by 325 publications

References 41 publications

From Function to Shape: A Novel Role of a Formin in Morphogenesis of the FungusAshbya gossypii

From Function to Shape: A Novel Role of a Formin in Morphogenesis of the FungusAshbya gossypii

A role for the Pcl9‐Pho85 cyclin–cdk complex at the M/G₁ boundary in Saccharomyces cerevisiae

Comprehensive Identification of Cell Cycle–regulated Genes of the YeastSaccharomyces cerevisiaeby Microarray Hybridization

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Asymmetric Accumulation of Ash1p in Postanaphase Nuclei Depends on a Myosin and Restricts Yeast Mating-Type Switching to Mother Cells

Cited by 325 publications

References 41 publications

From Function to Shape: A Novel Role of a Formin in Morphogenesis of the FungusAshbya gossypii

From Function to Shape: A Novel Role of a Formin in Morphogenesis of the FungusAshbya gossypii

A role for the Pcl9‐Pho85 cyclin–cdk complex at the M/G1 boundary in Saccharomyces cerevisiae

Comprehensive Identification of Cell Cycle–regulated Genes of the YeastSaccharomyces cerevisiaeby Microarray Hybridization

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A role for the Pcl9‐Pho85 cyclin–cdk complex at the M/G₁ boundary in Saccharomyces cerevisiae