Astragaloside IV prevents MPP+-induced SH-SY5Y cell death via the inhibition of Bax-mediated pathways and ROS production

Abstract

Parkinson's disease (PD) is characterized by a progressive degeneration of dopaminergic neurons in the substantia nigra. Oxidative stress and neural degeneration are suggested to be involved in the pathogenesis of PD. Previous studies have revealed that Astragaloside IV (AS-IV) can reduce inflammation and oxidation, making it a potential therapeutic agent for neurodegenerative disease. In this study, we investigated whether AS-IV protect against 1-methyl-4-phenylpyridnium ion (MPP(+))-induced dopaminergic neurotoxicity in SH-SY5Y cells and determined the mechanism of AS-IV neuroprotection. We found that pretreatment with AS-IV significantly reversed the loss of cell viability, nuclear condensation, the generation of intracellular reactive oxygen species (ROS), and the increase in Bax/Bcl-2 ratio and the activity of caspase-3 induced by MPP(+). Our study suggests that the neuroprotective effect of AS-IV is related to mechanisms including ROS production and the inhibition of Bax-mediated pathway. The present study supports the notion that AS-IV may be a promising neuroprotective agent for the treatment of neurodegenerative disorders such as PD.

Neuroprotective effects of astragaloside IV on Parkinson disease models of mice and primary astrocytes

“…A recent study reported that in SH-SY5Y cells, AS-IV significantly reversed MPP+-induced elevated activity of caspase-3 (35). We carried out western blot to investigate the effects of AS-IV on caspase-3 in astrocytes.…”

Astragaloside IV rescues MPP+-induced mitochondrial dysfunction through upregulation of methionine sulfoxide reductase A

“…In a previous study, AS-IV was demonstrated to exert notable protective effects against MPP + -induced oxidative damage in SH-SY5Y neuroblastoma cells (41). However, the antioxidant mechanism of AS-IV remains unclear.…”

“…Rg1 activates the Wnt/β-catenin signaling pathway in vivo and in vitro, thus exerting a protective effect against neurodegeneration (38). AS-IV has been used as an effective treatment for ischemia-reperfusion injury, myocardial infarction and type 2 diabetes (21,39,41). The protective functions of AS-IV are attributed to an increased cellular content and activity of superoxide dismutase (SOD), and the inhibition of calcium pumps.…”

Mimosa Pudica Exerts Neuroprotection Against Mpp+induced Neurotoxicity in Shsy5y Cell Lines-an in Vitro Model of Anti-Parkinsonism

“…Human neuroblastoma SH-SY5Y cells (NCCS, Pune), possess morphological, biochemical, and electrophysiological characteristics of dopaminergic neurones and have been widely used in the study of cell model for PD [7]. Cells were cultured in DMEM+F12 supplemented with 10% (v/v) heat-inactivated foetal calf serum and 100 units/ml penicillin/streptomycin.…”