Asticcacaulis solisilvae sp. nov., isolated from forest soil

Abstract

An obligately aerobic, chemoheterotrophic, mesophilic prosthecate bacterium, designated strain CGM1-3EN T , was isolated from the enrichment cultures of forest soil from Cheonggyesan Mountain, Republic of Korea. Cells were Gram-reaction-negative, motile rods (1.3-2.4 mm long by 0.30-0.75 mm wide) with single flagella. The strain grew at 10-37 6C (optimum 25-30 6C) and at pH 4.5-9.5 (optimum 5.0-7.0). The major cellular fatty acids were C 16 : 0 , C 18 : 1 v7c 11-methyl, C 12 : 1 3-OH and summed feature 8 (comprising C 18 : 1 v7c/C 18 : 1 v6c). The genomic DNA G+C content of strain CGM1-3EN T was 63.7 mol%. The closest phylogenetic neighbour to strain CGM1-3EN T was identified as Asticcacaulis biprosthecium DSM 4723 T (97.2 % 16S rRNA gene sequence similarity) and the DNA-DNA hybridization value between strain CGM1-3EN T and A. biprosthecium DSM 4723 T was less than 24.5 %. Strain CGM1-3EN T used Dglucose, D-fructose, sucrose, maltose, trehalose, D-mannose, D-mannitol, D-sorbitol, D-galactose, cellobiose, lactose, raffinose, fumarate, pyruvate, DL-alanine and glycerol as carbon sources. Based on data from the present polyphasic study, the forest soil isolate CGM1-3EN T is considered to represent a novel species of the genus Asticcacaulis, for which the name Asticcacaulis solisilvae sp. nov. is proposed. The type strain is CGM1-3EN T

Declining diversity of egg-associated bacteria during development of naturally spawned whitefish embryos (Coregonus spp.)

“…The most common bacterial taxa on early whitefish eggs that matched to a previously reported reference according to a search in Web Of Knowledge TM comprised mainly free-living freshwater bacteria, such as the genera of planktonic Hydrogenophaga (Willems et al 1989), Polynucleobacter , or the facultatively autotrophic genus Sulfuritalea (Kojima and Fukui 2011). Four ubiquitous bacterial groups that contributed with many sequences to the bacterial communities on early whitefish eggs have previously mostly been found in soil samples: Asticcacaulis (Kim et al 2013), Hyphomonadaceae (Lee et al 2005), Isosphaeraceae (Joseph et al 2003), and Mycoplana (Urakami et al 1990). Another family that was fairly common was the family of Saprospiraceae, which contains many genera of aquatic cosmopolitans (Schauer et al 2005).…”

Burkholderia jirisanensis sp. nov., isolated from forest soil

“…Strain JRM2-1 T is clearly distinguished from the BCC and belongs to group A in all of the phylogenetic trees (Fig. 1) DNA-DNA hybridization of strain JRM2-1 T against B. terrae KCTC 12388 T was performed as described previously (De Ley et al, 1970;Huss et al, 1983;Kim et al, 2013). The low DNA-DNA hybridization value (14.4 %) found between strain JRM2-1 T and B. terrae KCTC 12388…”

“…Strain JRM2-1 T and the reference strains were inoculated into independent cultures at the same time. Fatty acid methyl esters were prepared and analysed as described previously (Kim et al, 2013). The cellular fatty acids were extracted and prepared as fatty acid methyl esters, and the extracted fatty acid methyl esters were analysed using the Microbial Identification System (MIDI).…”

“…Polar lipids were separated as described previously (Kim et al, 2013;Komagata & Suzuki, 1987;Minnikin et al, 1984) using two-dimensional TLC. The separated polar lipids were stained with molybdatophosphoric acid, molybdenum blue, naphthol and ninhydrin for detection of total lipids, phospholipids, glycolipids and aminolipids, respectively.…”

“…The DNA G+C contents of strain JRM2-1 T and the reference strains were determined as described previously (Kim et al, 2013;Mesbah et al, 1989). The genomic DNA G+C content of strain JRM2-1 T was 63.7 mol% ( Table 1).…”