Astaxanthin Protects Dendritic Cells from Lipopolysaccharide-Induced Immune Dysfunction

Yin, Yinyan; Xu, Nuo; Zhou, Bin; Sun, Dongrui; Ma, Bixia; Xu, Zhengzhong; Yang, Jin; Li, Chunmei

doi:10.3390/md19060346

Cited by 23 publications

(18 citation statements)

References 53 publications

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“…TGF-β is required for IL-17 to produce T helper cell (Th-17 cell) differentiation [ 52 ]. In our data, astaxanthin reduced the production of IL-1β, IL-17, and TGF-β in LPS-induced DCs and in LPS-challenged mice, which is in line with our previous findings that showed a decrease in TNF-α, IL-6, and IL-10 caused by astaxanthin in an LPS-induced DC model [ 31 ]. These data suggest that astaxanthin, as an antioxidant, can effectively mitigate overloaded cytokine production in vitro and in vivo.…”

Section: Discussionsupporting

confidence: 93%

“…Previously, we found that astaxanthin strongly inhibited the immune dysfunction of DCs induced by LPS [ 31 ]. Here, our work further shows the antioxidative effects of astaxanthin in DCs and mice, which is a potential key aspect of inflammatory control in the sepsis model ( Figure 9 ).…”

Section: Discussionmentioning

confidence: 99%

“…C57BL/6 mice, aged 6 weeks old, were randomly divided into five groups ( n = 10/group), as described previously [ 31 ]. Astaxanthin (50, 100, and 200 mg/kg body weight) was orally administered for 2 days every 24 h; 48 h after the 1st oral administration, the mice were injected intraperitoneally with LPS (20 mg/kg body weight).…”

Section: Methodsmentioning

confidence: 99%

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Astaxanthin Provides Antioxidant Protection in LPS-Induced Dendritic Cells for Inflammatory Control

Yin

Qin

et al. 2021

Marine Drugs

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Astaxanthin, originating from marine organisms, is a natural bioactive compound with powerful antioxidant activity. Here, we evaluated the antioxidant ability of astaxanthin on dendritic cells (DCs), a key target of immune regulation, for inflammatory control in a sepsis model. Our results showed that astaxanthin suppressed nitric oxide (NO) production, reactive oxygen species (ROS) production, and lipid peroxidation activities in LPS-induced DCs and LPS-challenged mice. Moreover, the reduced glutathione (GSH) levels and the GSH/GSSG ratio were increased, suggesting that astaxanthin elevated the level of cellular reductive status. Meanwhile, the activities of antioxidant enzymes, including glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD), were significantly upregulated. Astaxanthin also inhibited the LPS-induced secretions of IL-1β, IL-17, and TGF-β cytokines. Finally, we found that the expressions of heme oxygenase 1 (HO-1) and nuclear factor erythroid 2-related factor 2 (Nrf2) were significantly upregulated by astaxanthin in LPS-induced DCs, suggesting that the HO-1/Nrf2 pathway plays a significant role in the suppression of oxidative stress. These results suggested that astaxanthin possesses strong antioxidant characteristics in DC-related inflammatory responses, which is expected to have potential as a method of sepsis treatment.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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Astaxanthin Provides Antioxidant Protection in LPS-Induced Dendritic Cells for Inflammatory Control

Yin

Qin

et al. 2021

Marine Drugs

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“…Next, CD69 was detected as a critical activation marker of DCs [23]. The results showed that the expression of CD69 in DCs was remarkably increased after treatment with riboflavin for 24 h (Figure 5A,B).…”

Section: Riboflavin Enhanced the Cytokine Production Of Dcsmentioning

confidence: 91%

Riboflavin as a Mucosal Adjuvant for Nasal Influenza Vaccine

Yin

Wang

et al. 2021

Vaccines

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Intranasal immunization with whole inactivated virus (WIV) is an important strategy used for influenza prevention and control. However, a powerful mucosal adjuvant is required to improve nasal vaccine efficacy. Riboflavin, as a food additive with the advantages of being safe and low-cost, widely exists in living organisms. In this paper, the mucosal adjuvant function of riboflavin was studied. After intranasal immunization with H1N1 WIV plus riboflavin in mice, we found that the mucosal immunity based on the secretory IgA (sIgA) levels in the nasal cavity, trachea, and lung were strongly enhanced compared with H1N1 WIV alone. Meanwhile, the IgG, IgG1, and IgG2a levels in serum also showed a high upregulation and a decreased ratio of IgG1/IgG2a, which implied a bias in the cellular immune response. Moreover, riboflavin strongly improved the protection level of H1N1 inactivated vaccine from a lethal influenza challenge. Furthermore, riboflavin was found to possess the capacity to induce dendritic cell (DC) phenotypic (MHCII, CD40, CD80, and CD86) and functional maturation, including cytokine secretion (TNF-α, IL-1β, IL-12p70, and IL-10) and the proliferation of allogeneic T cells. Lastly, we found that the DC maturation induced by riboflavin was dependent on the activation of the mitogen-activated protein kinase (MAPK) signaling pathway, which plays an important role in immune regulation. Therefore, riboflavin is expected to be developed as an alternative mucosal adjuvant for influenza nasal vaccine application.

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“…Marine carotenoids exert strong antioxidant, restorative, antiproliferative and anti-inflammatory effects and can be used both as skin photoprotection to inhibit the negative effects of solar UV radiation and as nutraceutical/cosmeceutical ingredients to prevent oxidative stress-related diseases. In this regard, Yin et al (2021) contributed with the publication of two research articles focused on the biological properties of astaxanthin, a naturally occurring red carotenoid pigment classified as xanthophyll native to marine organisms, such as microalgae and seafood, with known potent antioxidant properties [5,6]. The authors evaluated the anti-inflammatory and antioxidant capacity of astaxanthin on the immune functions of murine dendritic cells (DCs) in a sepsis model.…”

mentioning

confidence: 99%

Antioxidant and Anti-Inflammatory Agents from the Sea: A Molecular Treasure for New Potential Drugs

Vasarri

Degl’Innocenti

2022

Marine Drugs

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Astaxanthin Protects Dendritic Cells from Lipopolysaccharide-Induced Immune Dysfunction

Cited by 23 publications

References 53 publications

Astaxanthin Provides Antioxidant Protection in LPS-Induced Dendritic Cells for Inflammatory Control

Astaxanthin Provides Antioxidant Protection in LPS-Induced Dendritic Cells for Inflammatory Control

Riboflavin as a Mucosal Adjuvant for Nasal Influenza Vaccine

Antioxidant and Anti-Inflammatory Agents from the Sea: A Molecular Treasure for New Potential Drugs

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