Astacin‐like metallo‐endopeptidase is dynamically expressed in embryonic stem cells and embryonic epithelium during morphogenesis

Acloque, Hervé; Lavial, Fabrice; Pain, Bertrand

doi:10.1002/dvdy.23737

Cited by 10 publications

(7 citation statements)

References 40 publications

(56 reference statements)

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“…Evolutionary analysis of astacin-like metalloproteases in the medaka and zebrafish genomes [16] demonstrated that one or two genes represent bona fide “hatching enzymes”, while the rest are paralogous copies of still unclear function. A recent study [71] revealed that these proteins are involved in mouse and chicken embryogenesis and organogenesis, mostly in remodelling epithelia. Whether astacin-like metallo-endopeptidases have a similar role in the common sole and, more specifically, in promoting cellular/tissue migration through ECM reorganisation during early development represents an interesting topic for further studies.…”

Section: Discussionmentioning

confidence: 99%

Exploring the larval transcriptome of the common sole (Solea solea L.)

et al. 2013

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BackgroundThe common sole (Solea solea) is a promising candidate for European aquaculture; however, the limited knowledge of the physiological mechanisms underlying larval development in this species has hampered the establishment of successful flatfish aquaculture. Although the fact that genomic tools and resources are available for some flatfish species, common sole genomics remains a mostly unexplored field. Here, we report, for the first time, the sequencing and characterisation of the transcriptome of S. solea and its application for the study of molecular mechanisms underlying physiological and morphological changes during larval-to-juvenile transition.ResultsThe S. solea transcriptome was generated from whole larvae and adult tissues using the Roche 454 platform. The assembly process produced a set of 22,223 Isotigs with an average size of 726 nt, 29 contigs and a total of 203,692 singletons. Of the assembled sequences, 75.2% were annotated with at least one known transcript/protein; these transcripts were then used to develop a custom oligo-DNA microarray. A total of 14,674 oligonucleotide probes (60 nt), representing 12,836 transcripts, were in situ synthesised onto the array using Agilent non-contact ink-jet technology. The microarray platform was used to investigate the gene expression profiles of sole larvae from hatching to the juvenile form. Genes involved in the ontogenesis of the visual system are up-regulated during the early stages of larval development, while muscle development and anaerobic energy pathways increase in expression over time. The gene expression profiles of key transcripts of the thyroid hormones (TH) cascade and the temporal regulation of the GH/IGF1 (growth hormone/insulin-like growth factor I) system suggest a pivotal role of these pathways in fish growth and initiation of metamorphosis. Pre-metamorphic larvae display a distinctive transcriptomic landscape compared to previous and later stages. Our findings highlighted the up-regulation of gene pathways involved in the development of the gastrointestinal system as well as biological processes related to folic acid and retinol metabolism. Additional evidence led to the formation of the hypothesis that molecular mechanisms of cell motility and ECM adhesion may play a role in tissue rearrangement during common sole metamorphosis.ConclusionsNext-generation sequencing provided a good representation of the sole transcriptome, and the combination of different approaches led to the annotation of a high number of transcripts. The construction of a microarray platform for the characterisation of the larval sole transcriptome permitted the definition of the main processes involved in organogenesis and larval growth.

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Section: Discussionmentioning

confidence: 99%

Exploring the larval transcriptome of the common sole (Solea solea L.)

et al. 2013

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“…To date, SAS1B has been proposed to function in blastocyst hatching [ 43 ], sperm-egg interaction [ 1 ], the block to polyspermy [ 3 ], epithelial cell behaviour during early development in chick [ 44 ], and as a secretory protein released by oocyte activation [ 45 ]. Previously proposed translational applications of ASTL include its use as an ovarian cyst fluid biomarker [ 46 ] and target for an oocyte-specific contraceptive [ 2 ].…”

Section: Discussionmentioning

confidence: 99%

Membrane associated cancer-oocyte neoantigen SAS1B/ovastacin is a candidate immunotherapeutic target for uterine tumors

et al. 2015

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The metalloproteinase SAS1B [ovastacin, ASTL, astacin-like] was immunolocalized on the oolemma of ovulated human oocytes and in normal ovaries within the pool of growing oocytes where SAS1B protein was restricted to follicular stages spanning the primary-secondary follicle transition through ovulation. Gene-specific PCR and immunohistochemical studies revealed ASTL messages and SAS1B protein in both endometrioid [74%] and malignant mixed Mullerian tumors (MMMT) [87%] of the uterus. A MMMT-derived cell line, SNU539, expressed cell surface SAS1B that, after binding polyclonal antibodies, internalized into EEA1/LAMP1-positive early and late endosomes. Treatment of SNU539 cells with anti-SAS1B polyclonal antibodies caused growth arrest in the presence of active complement. A saporin-immunotoxin directed to SAS1B induced growth arrest and cell death. The oocyte restricted expression pattern of SAS1B among adult organs, cell-surface accessibility, internalization into the endocytic pathway, and tumor cell growth arrest induced by antibody-toxin conjugates suggest therapeutic approaches that would selectively target tumors while limiting adverse drug effects in healthy cells. The SAS1B metalloproteinase is proposed as a prototype cancer-oocyte tumor surface neoantigen for development of targeted immunotherapeutics with limited on-target/off tumor effects predicted to be restricted to the population of growing oocytes.

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“…Additionally, until now REX1 (ZPF42) and UTF1 have not been found in the avian genome. Therefore, genes expressed in undifferentiated, long-term proliferating cESCs include SOX3, POUV (POU5F3), DNMT3B, EPCAM, CLDN1, VGLL2, EOMES, CFC1B, GATA5, NANOG, ESRP2, OTX2, TRIM71, GATA4, FOXA2, LIN28A, ASTL, ENS-1, CDH1 and SALL4 (Jean et al, 2015;Vautherot et al, 2017;Acloque et al, 2012;unpublished data). These genes encode transcription factors, DNA demethylases, and cell adhesion molecules.…”

Section: Molecular Profilementioning

confidence: 99%

Pluripotency in avian species

Pain¹,

Kress²,

Rival‐Gervier³

2018

Int. J. Dev. Biol.

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Pluripotency defines the ability of a cell to self-renew and to differentiate into all embryonic lineages both in vitro and in vivo. This definition was first established mainly with the mouse model and the establishment of mouse embryonic stem cells (ESCs) in the 1980's and extended later on to other species including non-human primates and humans. Similarly, chicken ESCs were derived and established in vitro from pregastrulating embryos leading to cells with unique properties at molecular, epigenetic and developmental levels. By comparing the properties of murine, mammalian and avian ESCs and of the more recently discovered induced pluripotential stem (iPS)-derived cells generated in all of these species, avian specificities start to emerge including specific molecular genes, epigenetic mark profiles and original developmental properties. Here, we present common, but also avian-specific elements that contribute to defining avian pluripotency.

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Astacin‐like metallo‐endopeptidase is dynamically expressed in embryonic stem cells and embryonic epithelium during morphogenesis

Cited by 10 publications

References 40 publications

Exploring the larval transcriptome of the common sole (Solea solea L.)

Exploring the larval transcriptome of the common sole (Solea solea L.)

Membrane associated cancer-oocyte neoantigen SAS1B/ovastacin is a candidate immunotherapeutic target for uterine tumors

Pluripotency in avian species

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