Association of the interleukin-1 * genetic polymorphism and gastric cancer risk in Japanese

Guerra

et al. 2005

Helicobacter pylori cagA-positive strains and host cytokine proinflammatory polymorphisms have been associated with gastric carcinoma. However, the individual role of each factor has not been evaluated yet. Our aim was to evaluate whether IL-1 gene cluster and tumor necrosis factor-a (TNFA)-307 polymorphisms, as well as cagA-positive status, are associated with gastric carcinoma in a non-Caucasian population by analyzing the data in logistic regression models. We evaluated 166 patients with noncardia gastric carcinoma and 541 blood donors. Among them, 702 were successfully genotyped for all cytokine studied: 166 with gastric carcinoma and 536 controls. The carcinoma patients were considered to be H. pylori-positive if culture alone or 2 among preformed urease test, stained smear or histologic section, serology, polymerase chain reaction (PCR) for ureA and urea breath test were positive. In blood donors, H. pylori status was based on enzyme-linked immunosorbent assay. The cagA status was determined by PCR or serology. IL1B-511/-31, IL1RN (interleukin-1 receptor antagonist) and TNFA-307 polymorphisms were genotyped by PCR, PCR with restriction fragment length polymorphism, or PCR with confronting 2-pair primers. We found that the IL1RN2 polymorphic allele (OR 5 1.93) was associated with noncardia gastric carcinoma, even after inclusion of age, gender and cagA status in the logistic models. However, the cagA-positive status was the strongest independent factor associated with gastric carcinoma (OR 5 11.89). The other polymorphisms were not significantly associated with the disease when they were evaluated in logistic models. This study provides evidence supporting the independent associations of cagA-positive H. pylori status and IL1RN polymorphisms with noncardia gastric carcinoma. ' 2005 Wiley-Liss, Inc.

show abstract

Section: Discussionmentioning

confidence: 85%

IL1RN polymorphic gene and cagA‐positive status independently increase the risk of noncardia gastric carcinoma

Guerra

et al. 2005

show abstract

“…The C allele was designated if 2 bands of 88 and 67 bp were obtained and the T allele was designated if a single band of 155 bp was obtained. 22 The PCR products for tandem repeat polymorphisms of IL-1RN were used directly for electrophoresis. The IL-1RN gene has a pentaallelic 86 bp tandem repeat (VNTR) at intron 2, 26 and the alleles were coded conventionally as follows: allele 1, 4 repeats; allele 2, 2 repeats; allele 3, 5 repeats; allele 4, 3 repeats; and allele 5, 6 repeats (Table II).…”

Section: Genotyping Of Il-1a Il-1b and Il-1rnmentioning

confidence: 99%

“…However, ethnicity might greatly affect genotype status, and 2 recent studies revealed no difference in IL-1B-511 genotypes between GC and controls in Japanese and Taiwanese populations. 22,23 Even within Asia, the IL-1B polymorphisms differ between northern (Japanese and Chinese) and southern (Thai and Vietnamese) populations. In subjects with severe gastric mucosal atrophy, the IL-1B-511C/C polymorphism was dominant in the Japanese population and the IL-1B-511T/T ϩ T/C polymorphism was dominant in the Chinese population; however, no differences in C-and T-allele frequencies were found in the Thai and Vietnamese populations.…”

Section: Populationmentioning

confidence: 99%

“…20,21 In contrast, 2 recent studies could not find any association between the IL-1B-511 polymorphism and GC in a Japanese population and a Taiwanese population. 22,23 The aims of this study were to elucidate the association of IL-1A, IL-1B and IL-1RN loci polymorphisms as host genetic factors with the increased risk of developing GC in a Korean population and to examine the combined effect of IL-1 polymorphisms with subsequent enhanced gastric mucosal levels of IL-1␤ and H. pylori infection in gastric carcinogenesis.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Interleukin‐1B (IL‐1B) polymorphisms and gastric mucosal levels of IL‐1β cytokine in Korean patients with gastric cancer

Chang

Jang

Kim

et al. 2004

Interleukin-1B and IL-1 receptor antagonist gene polymorphisms are associated with an increased risk of gastric cancer (GC) in Caucasian populations. However, recent studies could not find any association between IL-1B-511T polymorphism and the risk of GC in Asians. We tested for an association between IL-1 loci polymorphisms with increased gastric mucosal levels of IL-1␤ and an increased risk of developing GC in a Korean population. Polymorphisms of IL-1A-889, IL-1B-31, IL-1B-511 and IL-1RN were genotyped in 434 controls and 234 patients with GC. Mucosal IL-1␤ cytokine was measured using an ELISA. The frequencies of IL-1A, IL-1B-511, IL-1B-31 and IL-1RN were not statistically different between controls and all patients with GC. After subclassification of GC, only patients with intestinal-type GC showed a higher frequency of IL-1B-31T homozygotes (OR ‫؍‬ 2.2; 95% CI ‫؍‬ 1.1-4.3) compared with controls. Risk was also significantly increased in these patients for IL-1B-31T homozygotes compared with patients with diffuse-type GC (OR ‫؍‬ 3.4; 95% CI ‫؍‬ 1.5-7.7). As in Caucasian populations, linkage disequilibrium between IL-1B-31 and IL-1B-511 was nearly complete, but the pattern of haplotype related to the risk of GC (IL-1B-31T/IL-1B-511C) was opposite (IL-1B-511T/IL-1B-31C). Mucosal IL-1␤ levels in H. pylori-infected GC patients were higher in patients homozygous for IL-1B-31T compared with IL-1B-31C/T and IL-1B-31C/C. Thus, the combined effects of H. pylori infection and IL-1B-31T/IL-1B-511C polymorphisms with enhanced mucosal IL-1␤ production contributed to the development of intestinal-type GC in this Korean population.

show abstract

“…20,21 It is becoming evident, however, that even in these Asian populations, one has to consider the background incidence of the cancer in question. Thus, Zeng et al 22 reported recently that IL-1B-511T/T genotype was a significant risk factor for gastric cancer in low incidence areas of China but that the effect of this genotype was less obvious in areas of high incidence.…”

Section: Il-1b-31(c/t) Tnf-a-308mentioning

confidence: 99%

Role of the polymorphic IL‐1B, IL‐1RN and TNF‐A genes in distal gastric cancer in Mexico

Garza‐González

Bosques-Padilla

El-Omar

et al. 2004

109