Association of SNP in FTO gene, rs9939609, with metabolic syndrome at a tertiary care unit of Karachi, Pakistan

Abstract

Objective:To determine the association of SNP in FTO gene, rs9939609, with Metabolic Syndrome (MS) in type 2 diabetic subjects at a tertiary care unit of Karachi, Pakistan.Methods:We genotyped FTO rs9939609 SNP in 296 patients with type 2 diabetes from the Out Patient Department (OPD) of Baqai Institute of Diabetology and Endocrinology (BIDE). MS was defined on the basis of International Diabetes Federation (IDF) and National Cholesterol Education program (NCEP) criterion. Association between the rs9939609 SNP and MS was tested through chi-square and Z-tests by using odds ratio (OR) with 95% confidence intervals.Results: The frequency of MS as defined by IDF criterion was significantly higher in female subjects as compared to male subjects (p= 0.006). Carriers of ≥ 1 copy of the rs9939609 A allele were significantly more likely to had MS (69.6%) than non-carriers (30.4%), corresponding to a carrier odds ratio (OR) of 0.52 (95% confidence interval [CI] (0.29-0.93), with a similar trend for the ATP III-defined MS.“A” allele carriers under dominant model, carry all the criterion of MS more significantly as compared to non-carriers.Conclusion:The FTO rs9939609 SNP was associated with an increased risk for Metabolic Syndrome in type 2 diabetic populations at a tertiary care unit of Karachi, Pakistan.

Obesity in the Balinese is associated with FTO rs9939609 and rs1421085 single nucleotide polymorphisms

“…The FTO rs9939609 and rs1421085 variants were detected using amplification-refractory mutation system (ARMS) polymerase chain reaction (PCR). Detection of rs9939609 was performed using a previously published primer sets (Fawwad et al, 2015) while detection of rs1421085 was done using a novel primer sets, designed using Primer1 (Collins & Ke, 2012) and BioEdit Ò Sequence Alignment Editor (Ibis Bioscience, Carlsbed, CA, USA). Lists of outer and inner primers for ARMS-PCR is described in Table S1.…”

Influence of FTO rs9939609 and Mediterranean diet on body composition and weight loss: a randomized clinical trial

“…Subsequently, further studies demonstrated that the allele A of FTO rs9939609 is related to both a higher body mass index and body circumferences [ 21 , 22 ], and, among different ethnicities, also Italian population demonstrated the same results [ 23 ]. Furthermore, this SNP is included among the genetic etiological factors in the development of both metabolic syndrome and Type-2 diabetes [ 24 , 25 ], and allele A was also associated with a higher fat and lean mass [ 26 , 27 ]. In a PREDIMED substudy, it was observed that homozygous subjects for A allele had the highest baseline body weight, but also the lowest body weight gain after 3 years of Mediterranean-style intervention, compared to TT genotype.…”

Peer Review #2 of "Obesity in the Balinese is associated with FTO rs9939609 and rs1421085 single nucleotide polymorphisms (v0.1)"

“…The FTO rs9939609 and rs1421085 variants were detected using amplification-refractory mutation system (ARMS) polymerase chain reaction (PCR). Detection of rs9939609 was performed using a previously published primer sets (Fawwad et al, 2015) while detection of rs1421085 was done using a novel primer sets, designed using Primer1 (Collins & Ke, 2012) and BioEdit® Sequence Alignment Editor (Ibis Bioscience, Carlsbed, CA, USA). Lists of outer and inner primers for ARMS-PCR is described in Supplementary Table S1.…”

“…Among the significant obesity genetic risk factors, the common single nucleotide polymorphisms (SNPs) rs9939609 and rs1421085 in the fat mass and obesity-associated (FTO) gene have been consistently reported to be associated with obesity in distinct populations (Chang et al, 2008;Cha et al, 2008;Fawwad et al, 2015;Babenko et al, 2019).…”