Association of single nucleotide polymorphisms of ERCC1 and XPF with colorectal cancer risk and interaction with tobacco use

ABSTRACT. We conducted a study to investigate the association between ERCC1 (rs3212986) and ERCC2 (rs13181) gene polymorphisms and the risk of pancreatic cancer in a Chinese population. A total of 217 pancreatic cancer patients and 244 control subjects were recruited from the Nuclear Industry 215 Hospital of Shaanxi Province between February 2013 and December 2014. Genomic DNA was extracted from peripheral blood samples using a TIANamp Blood DNA Kit (Tiangen, Beijing, China) according to the manufacturer's instructions. The ERCC1 rs3212986 and ERCC2 rs13181 polymorphisms were genotyped by polymerase chain reaction-restriction fragment length of polymorphism. Unconditional logistic regression analyses showed that subjects with the CC genotype of ERCC1 rs3212986 were susceptible to the development of pancreatic cancer when compared with subjects with the AA genotype (OR = 2.57, 95%CI = 1.34-5.02). The ERCC1 rs3212986 gene polymorphism was associated with increased risk of pancreatic cancer in the dominant (OR = 1.54, 95%CI = 1.05-2.28) and recessive (OR = 2.22, 95%CI = 1.20-4.19) models. However, no significant difference was found between the ERCC2 rs13181 polymorphism and the risk of pancreatic cancer in the codominant, dominant, and recessive models. We suggest that the ERCC1 rs3212986 polymorphism increases susceptibility to pancreatic cancer in the codominant, dominant, and recessive models, although further studies are needed to confirm our findings.

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Association between ERCC1 and ERCC2 gene polymorphisms and susceptibility to pancreatic cancer

He¹,

Zheng²,

Tan³

et al. 2016

“…Many epidemiologic studies have investigated the impact of the ERCC1 rs3212986 polymorphism in the risk of several cancers including glioma, breast cancer, lung cancer, and colorectal cancer (Adel Fahmideh et al, 2014;Zhu et al, 2014;Hou et al, 2014). For example, Adel Fahmideh et al (2014) conducted a meta-analysis of 27 case-control studies and assessed the association between DNA repair gene polymorphisms and risk of glioma, and found that the ERCC1 rs3212986 polymorphism may significantly increase the risk of glioma.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, Zhu et al (2014) conducted a meta-analysis of 16 case-control studies, which demonstrated that there was no association between the ERCC1 rs3212986 polymorphism and lung cancer. However, Hou et al (2014) conducted a 1:1 matched case-control study in a Chinese population, and found that the ERCC1 rs3212986 polymorphism was correlated with an increased risk of colorectal cancer. Taken together, these previous findings suggest that the ERCC1 rs3212986 polymorphism has a role in the development of certain cancers, which may be dependent on the study population.…”

Section: Discussionmentioning

confidence: 99%

Association between ERCC1 and ERCC2 polymorphisms and breast cancer risk in a Chinese population

Zhao¹,

Mf²

2016

ABSTRACT. We conducted a case-control study to investigate the role of ERCC1 rs3212986 and ERCC2 rs13181 gene polymorphisms in the development of breast cancer. Between March 2012 and March 2014, a total of 242 newly diagnosed breast cancer patients with histopathologically confirmed primary breast cancer and 242 healthy controls were recruited. Genotyping of ERCC1 rs3212986 and ERCC2 rs13181 polymorphisms was carried out using polymerase chain reaction-restriction fragment length polymorphism analysis. Unconditional logistic regression analyses indicated that the TT genotype of rs3212986 was associated with a higher risk of breast cancer compared to that associated with the GG genotype (OR = 2.05, 95%CI = 1.13-3.78). In dominant and recessive models, we found that the rs3212986 polymorphism was associated with increased risk of breast cancer, and the ORs were 1.50 (95%CI = 1.03-2.18) and 1.74 (95%CI = 1.01-3.11), respectively. In summary, we found that the ERCC1 rs3212986 polymorphism was associated with the development of breast cancer.

“…Guo et al (2015) conducted a meta-analysis with 3308 patients and 3242 controls from eight studies, and reported that individuals with the ERCC1 rs3212986 polymorphism were associated with an increased risk of breast cancer in Caucasians. Hou et al (2014) conducted a study in a Chinese population, and reported that the ERCC1 rs3212986 polymorphism is associated with a risk of colorectal cancer. Zhu et al (2014) conducted a meta-analysis with 11 case-control studies, but they did not find any association between the ERCC1 rs3212986 polymorphism and development of lung cancer.…”

Section: Discussionmentioning

confidence: 99%

Role of single nucleotide polymorphisms of DNA repair genes in susceptibility to pancreatic cancer in Chinese population

Mf¹,

Zhao²

2016

ABSTRACT. We conducted a case-control study to investigate the role of ERCC1-ERCC5 gene polymorphisms in the risk of pancreatic cancer. This study included 195 patients who were newly diagnosed with histopathologically confirmed primary pancreatic cancer, and 254 controls were recruited from Sir Run Run Shaw Hospital, between January 2012 and December 2014. Genotyping of ERCC1 rs3212986 and rs11615, ERCC2 rs13181, ERCC3 rs4150441, ERCC4 rs6498486, and ERCC5 rs2094258 polymorphisms was carried out using polymerase chain reaction coupled with restriction fragment length polymorphism. Unconditional logistic regression analyses showed that the TT genotype of ERCC1 rs3212986 was associated with an increased risk of pancreatic cancer, and the OR (95%CI) was 2.26 (1.21-4.22). However, we did not find a significant association between ERCC1 rs11615, ERCC2 rs13181, ERCC3 rs4150441, ERCC4 rs6498486, and ERCC5 rs2094258 polymorphisms and risk of pancreatic cancer. In summary, we found that the presence of the ERCC1 rs3212986 polymorphism correlated with an increased risk of pancreatic cancer.