Association of micro<scp>RNA</scp>‐200c expression levels with clinicopathological factors and prognosis in endometrioid endometrial cancer

Wilczyński, Miłosz; Danielska, Justyna; Domańska-Senderowska, Daria; Dzieniecka, Monika; Szymańska, Bożena; Malinowski, Andrzej

doi:10.1111/aogs.13306

Cited by 20 publications

(14 citation statements)

References 30 publications

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“…Wang et al [17] found that in primary or metastatic EC tissues, miR-29b was lower expressed compared with the normal controls, and using miR-29b expression to diagnose EC output 0.976 area under the curve (AUC), with the 96.1% sensitivity and 97.9% specificity, suggesting that lower expression of miR-29b correlated with poor prognosis of EC patients. Similarly, upregulation of miR-200c in endometrioid-EC tissues compared with normal controls was revealed by Wilczynski et al [18]; they also found that lower expression of miR-200c was linked to unfavorable survival.…”

Section: Introductionmentioning

confidence: 59%

Establishment of the Prognosis Predicting Signature for Endometrial Cancer Patient

Tang

Luo

2019

Med Sci Monit

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BackgroundNovel biomarkers provide clinicians more critical information on tumor genetic features and patients’ prognosis. Here, we aimed to establish prognosis-predicting signatures for endometrial carcinoma (EC) patients based on the miRNA information.Material/MethodsThe Cancer Genome Atlas (TCGA) website was available for dataset extraction. Prognosis-associated miRNAs were generated by univariate Cox regression test. Online websites were used to predict the targeted genes of these enrolled miRNAs. The miRNA-mRNA network was described by Cytoscape software, while the relevant signaling pathways of these targeted genes were enriched by Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses.ResultsThe miRNA-based overall survival (OS) and recurrence-free survival (RFS) predicting signatures were constructed by LASSO Cox regression analyses, respectively, by which, the endometrial carcinoma patients were separated into high- and low-risk groups in both the discovery and validation sets. Univariate Cox regression analyses suggested that these high-risk patients had elevated death and recurrence risk compared to low-risk patients. In addition, multivariate Cox regression analysis confirmed that our signatures were independent prognosticate factors with or without clinicopathological features for endometrial carcinoma patients. Moreover, the miRNA-mRNA network was displayed by Cytoscape software, and the pathway enrichment analyses found that the targeted genes of these enrolled miRNAs were enriched in tumor progression and drug resistance-related pathways.ConclusionsThe OS and RFS predicting classifiers serve as independent prognosis-associated determiners for EC patients.

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Section: Introductionmentioning

confidence: 59%

Establishment of the Prognosis Predicting Signature for Endometrial Cancer Patient

Tang

Luo

2019

Med Sci Monit

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“…Lee et al [31] identified the microRNA expression profile related to lymph node status in women with early-stage grade 1–2 endometrial cancer. Wilczynski et al [32] reported aberrant microRNA expression in endometrial carcinoma using formalin-fixed paraffin-embedded (FFPE) tissues. In this study, we found that miR-369-3p was significantly downexpressed in EEC tissues compared to control tissues and that the upregulation of miR-369-3p inhibited EEC cell proliferation via ATG10.…”

Section: Discussionmentioning

confidence: 99%

MiR-369-3p participates in endometrioid adenocarcinoma via the regulation of autophagy

Liu

et al. 2019

Cancer Cell Int

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Background The aim of this study is to examine miRNA profiling and miR-369-3p participates in endometrioid adenocarcinoma (EEC) via the regulation of autophagy. Methods EEC and its adjacent normal tissues were obtained from 20 clinical patients after surgery. MiRNA profiling was performed using next generation sequencing (NGS) and was validated with quantitative real time PCR (qRT-PCR). qRT-PCR was also employed to measure miR-369-3p and autophagy-related protein 10 (ATG10) expression levels. Western blotting assay was performed to measure the expressions of ATG10 and LC3B. Luciferase reporter assay was conducted to confirm the direct targeting of ATG10 by miR-369-3p. Cell proliferation and migration assays were utilized to analyze the role of miR-369-3p in HEC-1-A cells. Results We found that miR-369-3p expression levels were down-regulated in EEC compared to the control tissues. The overexpression of miR-369-3p inhibited cell proliferation and migration in EEC; furthermore, ATG10 expression increased in EEC tissues. ATG10 was found to be a potential target of miR-369-3p via a dual-luciferase reporter assay, and ATG10 was shown to be down-regulated by miR-369-3p in protein levels. Conclusions This study revealed that miR-369-3p inhibited cell proliferation and migration by targeting ATG10 via autophagy in EEC. Electronic supplementary material The online version of this article (10.1186/s12935-019-0897-8) contains supplementary material, which is available to authorized users.

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“…[26][27][28][29] However, there are few reports about miR-200c and cervical cancer. To date, no miRNA-4636-related pathway has been reported.…”

Section: Discussionmentioning

confidence: 99%

Peripheral blood circulating microRNA‐4636/−143 for the prognosis of cervical cancer

Yin

Yang

et al. 2019

J of Cellular Biochemistry

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Cervical cancer is the third leading cause of female death in the world. Serum microRNAs (miRNAs) are currently considered to be valuable as noninvasive cancer biomarkers, but their role in the prognosis of cervical cancer has not been elucidated. We aimed to find serum miRNAs that can be used as prognostic factors for cervical cancer. A traumatic pathological biopsy is the only reliable method for determining the severity of cervical cancer currently. Thus, noninvasive diagnostic markers are needed. The serological expression of candidate miRNAs were measured in 90 participants, including 60 patients with cervical cancer and 50 patients with cervical intraepithelial neoplasia. Two patients with cervical cancer were excluded from the study because of lack of data. miRNAs were evaluated by quantitative reverse transcription polymerase chain reaction. miR‐143/−4636 appeared specific for cervical cancer compared with cervical intraepithelial neoplasia (P < .001). The classification performance of validated miRNAs for cervical cancer [Area under the receiver operating characteristic curve (AUC) = 0.942] was better than that reached by squamous cell carcinoma antigen (SCC‐Ag; AUC = 0.727). Poor‐differentiation group has lower miR‐143/−4636 levels in serum (P < .05). miR‐4636 level was correlated gross tumor volume and the depth of invasion (P < .0001). In our study, we found a combination of miR‐143 and miR‐4636 that is independently and strongly associated with cervical cancer prognosis and can be used as a clinically prognostic factor.

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Association of microRNA‐200c expression levels with clinicopathological factors and prognosis in endometrioid endometrial cancer

Abstract: Expression levels of miRNA-200c might be associated with clinicopathological factors and survival in endometrioid endometrial cancer.

Cited by 20 publications

References 30 publications

Establishment of the Prognosis Predicting Signature for Endometrial Cancer Patient

Establishment of the Prognosis Predicting Signature for Endometrial Cancer Patient

MiR-369-3p participates in endometrioid adenocarcinoma via the regulation of autophagy

Peripheral blood circulating microRNA‐4636/−143 for the prognosis of cervical cancer

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