Association of mesenchymal stem cells with platelet rich plasma on the repair of critical calvarial defects in mice

Abstract

PURPOSE: To evaluate the effects of mesenchymal stem cells (MSC) from eight mice C57BL/6 gfp+ bone marrows expanded in cultures associated with platelets rich plasma (PRP) deriving from another eight mice, in the repair of critical defects in calvarial bone produced in twenty-four adult isogenic mice C57BL/6. METHODS: The animals were submitted to a cranial defect of 6.0mm in diameter and divided into two equal experimental groups. Control group did not receive treatment and the treated group received a MSC pellet containing 1.0 x 10(7) cells/mL associated with 50.0µL of plasma gel containing 1.0 x 10(9) autologous platelets within the defect. RESULTS: In the treated group was observed process of angiogenesis and bone repair better than control group. CONCLUSION: Mesenchymal stem cells derived from bone marrow of C57BL/6 gfp+ mice associated with PRP gel applied in bone critical defects produced in calvarial contributes positively to the process of bone repair.

Exploring anorganic bovine bone granules as osteoblast carriers for bone bioengineering: a study in rat critical-size calvarial defects

“…However, both Biomaterial and Biomaterial + Cells groups presented similar behavior: in both groups, it was possible to verify cells and new bone formation into natural pores of the granules, this last similar to those described in the literature (22). These results are interesting because these materials can be used as scaffold to osteoblasts in addition to promoting the recruitment of cells from the host tissue (22,23). In fact, providing the recruitment of specific cells, such as osteoprogenitor cells, is also one of the most important and decisive events in order to restore a new tissue (23).…”

“…Moreover, most studies in the literature have shown that the addition of cells, in combination or not with growth factors and transported by a biomaterial may favor the process of bone formation (22). Some studies have reported an increase in bone formation, accelerated by these components (cells and growth factors) in the initial period or shorter (1-2 weeks) (23). Probably for this reason, such difference was not observed in the Biomaterial + Cells group, since the evaluation period was 30 days.…”

“…These results are interesting because these materials can be used as scaffold to osteoblasts in addition to promoting the recruitment of cells from the host tissue (22,23). In fact, providing the recruitment of specific cells, such as osteoprogenitor cells, is also one of the most important and decisive events in order to restore a new tissue (23). Also similar results were found in this work, once there was formation of bone tissue within the pores of the material (Biomaterial and Biomaterial + Cells groups).…”

Bone Progenitors Produced by Direct Osteogenic Differentiation of the Unprocessed Bone Marrow Demonstrate High Osteogenic Potential In Vitro and In Vivo

“…Cell-plating time, seeding density, and preseeding or postseeding differentiation influence the bone repair efficacy of such constructs, making their comparison to BPC-seeded scaffolds unreliable. However, the percentage of a new bone (76.1%±18.3%) in the defects transplanted with PLA/PGA scaffolds seeded with about 100,000 BPCs was compatible to or even higher than in published studies of bone repair by MSCs in the calvarial model in mice (50,000 MSCs transplanted on PLA/PGA scaffolds 30 or 10 7 MSCs in platelet-rich plasma [PRP] 31 ), in rats (500,000 MSCs in agarose 32 or umbilical cord blood-derived predifferentiated MSCs mixed with a demineralized bone matrix 33 or 300,000 MSCs in chitosan 34 ), and in rabbits, where 2×10 7 MSCs were transplanted in PRP gel. In the latter study, MSCs predifferentiated with dexamethasone produced better results than undifferentiated MSCs.…”

Aspectos Radiográficos Do Sulco Intertroclear De Coelhos Submetidos Ao Enxerto Osteocondral Alógeno Associado À Terapia Com Células Mononucleares Autógenas E Dexametasona

“…Assim como no reparo de defeitos da tíbia de ovinos, Niemeyer et al (2010) notaram aumento significativo na neoformação óssea. Avaliação semelhante foi realizada por Monteiro et al (2012) na reparação de defeitos críticos em calvaria de camundongos por meio da associação de células--tronco mesenquimais da medula óssea com plasma rico em plaquetas. Neste experimento, por meio de avaliações das imagens radiográficas, notou-se que com a administração de células mononucleares autógenas intra-articular e dexametasona intramuscular, aumento significativo da neoformação óssea.…”