Abstract:The close association of the MPs and IRBP may suggest a functional role for this complex. As a practical consideration, it is likely that many preparations of IRBP may be contaminated with one or more MPs. We found no evidence for any other class of proteinase. Caution should thus be exercised in using an IRBP preparation purified from fresh tissue. It should be monitored for proteinase activity by zymography and/or prepared or stored in the presence of EDTA or dithiothreitol as much as possible.
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