Association of mast cells with tumor angiogenesis in esophageal squamous cell carcinoma

Tomita, Masaki; Matsuzaki, Yasunori; Edagawa, Masao; Shimizu, Tetsuya; Hara, Masaki; Sekiya, Ryou; Onitsuka, Toshio

doi:10.1046/j.1442-2050.2001.00171.x

Cited by 33 publications

(34 citation statements)

References 14 publications

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“…Among the various host immune cells, the mast cells have been implicated in tumour progression because they promote angiogenesis [5,14]. To examine the relationship between the mast cell density and the histological grade of OSCC, we analyzed the mast cell density (MCD) in different grades of OSCC and compared it with that of the normal mucosa by using the 1% Toluidine blue stain as the mast cells stain metachromatically with Toluidine blue.…”

Section: Introductionmentioning

confidence: 99%

The Relevance of Mast Cells in Oral Squamous Cell Carcinoma

Cheema¹,

Ramesh²,

Balamurali³

2012

JCDR

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Context:The commonly occurring oral neoplasm -Oral Squamous Cell Carcinoma (OSCC) has an association with the chronic inflammation in the adjacent connective tissue. Mast cells are the local residents of the connective tissue. They are said to be pro inflammatory and immune-amplifying in action and they produce mitogenic cytokines. They exhibit a phenotypic plasticity with a change in the microenvironment, thus making the role of the mast cells in this disease even more intriguing. Mast cells have also been reported to play a role in tumour progression and metastases by promoting angiogenesis.Aims: The present study aimed at comparing the Mast Cell Density (MCD) in the Normal Oral Mucosa (NM) and among the various grades of OSCC and at analyzing the possible role of the mast cells. Settings and Design:The MCD was assessed by using the 1% Toluidine blue stain in a study sample of 102 cases of different grades of OSCC and in 07 cases of clinically normal oral mucosa.Statistical Analysis Used: By using the Student's 't' test and one way ANOVA, the Mast Cell Density (MSD) was found to be significantly higher in well differentiated squamous cell carcinoma and in moderately differentiated squamous cell carcinoma as compared to that in the poorly differentiated oral squamous cell carcinoma (P=< 0.01) which was significant at a 1% level. The Spearman's Rank Correlation Co-efficient revealed a significant correlation between the density of the mast cells and the increasing grade of the OSCC. Conclusions:These findings denote that as the squamous cell carcinoma enters the poorly differentiated state, there may be some change which is reflected as a decrease in the mast cell density.

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Section: Introductionmentioning

confidence: 99%

The Relevance of Mast Cells in Oral Squamous Cell Carcinoma

Cheema¹,

Ramesh²,

Balamurali³

2012

JCDR

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“…This is reasonable, because LPA can function as a growth factor for cancer cells and also stimulates angiogenesis [34]. More than 40 years ago, it has been reported that a large number of MC accumulate around solid tumors and a large number of tumor-associated MC were significantly correlated with high vascular density and poor outcome [32,44,65]. In those studies, MC-derived secretalogues, such as histamine, heparin, or VEGF are considered to be responsible for the new vessel formation.…”

Section: Discussionmentioning

confidence: 97%

Submucosal connective tissue-type mast cells contribute to the production of lysophosphatidic acid (LPA) in the gastrointestinal tract through the secretion of autotaxin (ATX)/lysophospholipase D (lysoPLD)

et al. 2007

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Lysophosphatidic acid (LPA) is involved in a broad spectrum of biological activities, including wound healing and cancer metastasis. Autotaxin (ATX), originally isolated from a melanoma supernatant as a tumor cell motility-stimulating factor, has been shown to be molecularly identical to lysophospholipase D (lysoPLD), which is the main enzyme in the production of LPA. Although ATX/lysoPLD is known to be widely expressed in normal human tissues, the exact distribution of ATX-producing cells has not been fully investigated. In this study, we evaluated ATX/lysoPLD expression by immunohistochemical staining using a rat anti-ATX mAb in the human gastrointestinal tract and found that submucosal mast cells (MC) highly expressed this enzyme. This was confirmed by immunofluorescent double staining using mAbs to tryptase and chymase. Then, we isolated MC from human gastric tissue by an immunomagnetic method using CD117-microbeads and showed that a subpopulation of CD203c-positive MC showed positive staining for intracellular ATX/lysoPLD on flowcytometry. This was confirmed by Western blotting of the isolated cells. Moreover, a significant level of ATX/lysoPLD release could be detected in the culture supernatants of human MC by Western blot analysis. Our data suggest that submucosal MC play significant roles in various aspects of pathophysiology in the gastrointestinal tract by locally providing bioactive LPA through the production of ATX/lysoPLD.

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“…In this study, mast cells were found in close contact with fibroblasts. Mast cell activation is a characteristic feature of chronic inflammation, a condition that may lead to fibrosis as a result of increased collagen synthesis by a fibroblast [140][141][142][143][144].…”

Section: Cell-cell Interaction In Dermal Resident Cellsmentioning

confidence: 99%

“…Fibroblasts can modulate the functions of both mast cells and eosinophils, which also increase the amount of melanocytes and melanin pigment [141][142][143].…”

Section: Cell-cell Interaction In Dermal Resident Cellsmentioning

confidence: 99%

Cell-Cell Interactions and Cross Talk Described in Normal and Disease Conditions: Morphological Approach

Ayuob¹,

Ali²

2012

Cell Interaction

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Additional information is available at the end of the chapter http://dx.doi.org/10.5772/48435 IntroductionThe contact between cells and their microenvironment is fundamental both during development and for the preservation of tissue structure. Picking out the signals coming from the surrounding environment enable cells to react promptly to changes that may occur. Various molecular mechanisms explain the ability of cells to sense the microenvironment could be grouped into two major classes: (1) the transmission of signals in the form of soluble molecules which interact with cellular receptors, such as growth factors, cytokines, hormones, etc., and (2) the interaction of cells with structural components of their environment, namely other cells and the extracellular matrix (ECM) [1].Cell-cell interactions are central to the function of many organ systems. A common theme for heterotypic cell interactions is the interaction of parenchymal cells with nonparenchymal neighbors with resultant modulation of cell growth, migration, and/or differentiation. Specifically, these interactions are of fundamental importance in physiology [2,3], pathophysiology [4,5], cancer [6,7] developmental biology [8,9], wound healing [10,11], and attempts to replace tissue function through 'tissueengineering' [12,13]. Further understanding of how cell-cell interactions modulate tissue function will allow us to gain fundamental biological insight as well as suggest approaches that will allow the manipulation of tissue function in vitro for therapeutic applications in vitro for therapeutic applications [14]. Cell-cell interaction and cross talk phenomena during embryonic periodCells are the true miracle of evolution. Once the basic building block, the eukaryotic cell, became available, the form of metazoans evolved by changing the arrangement of cells with respect to each other. Cell-cell interaction in embryo was described in literature to have a

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Association of mast cells with tumor angiogenesis in esophageal squamous cell carcinoma

Cited by 33 publications

References 14 publications

The Relevance of Mast Cells in Oral Squamous Cell Carcinoma

The Relevance of Mast Cells in Oral Squamous Cell Carcinoma

Submucosal connective tissue-type mast cells contribute to the production of lysophosphatidic acid (LPA) in the gastrointestinal tract through the secretion of autotaxin (ATX)/lysophospholipase D (lysoPLD)

Cell-Cell Interactions and Cross Talk Described in Normal and Disease Conditions: Morphological Approach

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