Association of hyaluronic acid with a deproteinized bovine graft improves bone repair and increases bone formation in critical‐size bone defects

Matheus, Henrique Rinaldi; Ervolino, Edílson; Gusman, David Jonathan Rodrigues; Alves, Breno Edson Sendão; Fiorin, Luiz Guilherme; Pereira, Priscilla Aparecida

doi:10.1002/jper.20-0613

Cited by 20 publications

(13 citation statements)

References 51 publications

(111 reference statements)

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“…Similarly, HA promotes bone regeneration by influencing cell proliferation and osteogenic differentiation. In a review of the literature, it was found that HA could be positive for the expression of BMP-2 or other osteoprogenitor and osteogenic markers as it provides an ideal environment by virtue of its physicochemical properties (Nickel and Mueller, 2017;Matheus et al, 2020;Cabreira et al, 2021). In this study, the expression of BMP-2 was upregulated at 4 and 8 weeks.…”

Section: Discussionmentioning

confidence: 55%

Application of Hyaluronic Acid as a Biopolymer Material in Reconstruction of Interdental Papilla in Rats

Zhang

Hong

2021

Front. Mater.

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Applying hyaluronic acid, a biopolymer material, in the treatment of interdental papilla reconstruction has become a trend. The main objective of this research is to investigate the histologic effect of hyaluronic acid on interdental papilla over time. Deficient interdental papilla models were surgically created in sixty-two Sprague-Dawley (SD) rats and were randomly treated with the injection of hyaluronic acid (HA group) or phosphate-buffered saline (sham control group) or left untreated (control group). After 2, 4, and 8 weeks, the rats were sacrificed in batches to observe the histological changes. A fluorochrome label was used to monitor bone formation in 8 weeks. Immunohistochemical analyses were performed to evaluate the expression of potentially relevant cytokines, vascular endothelial growth factor (VEGF), bone morphogenetic protein-2 (BMP-2), and Wnt-induced secreted protein 1 (WISP1) in the gingival tissue in 8 weeks. A preliminary study of HA degradation after 24 weeks was performed in two rats. Following the HA injection, no inflammation or granulomatous foreign body reaction was observed. HA was able to promote collagen fiber and alveolar bone regular formation in the reconstruction site. HA also enhanced VEGF, BMP-2, and WISP-1 expression in gingival tissue (p＜0.05). After 24 weeks, there was no HA filler observed in the interdental papilla. In conclusion, our study suggested that HA is an effective way to reconstruct deficient interdental papilla.

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Section: Discussionmentioning

confidence: 55%

Application of Hyaluronic Acid as a Biopolymer Material in Reconstruction of Interdental Papilla in Rats

Zhang

Hong

2021

Front. Mater.

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“…In the groups treated with biomaterial, a significantly higher number of TRAP-positive cells was observed, possibly because of the reduction in the rate of resorption by osteoclasts during repair. Conflicting data are presented in these groups when combined with the greater amount of new bone, suggesting that accelerated resorption of graft granules before 30 days is not beneficial to bone formation, which was confirmed by the percentage of new bone tissue formed lower in the groups treated with clot at the end of the experiment [ 89 ].…”

Section: Discussionmentioning

confidence: 99%

Use of Photobiomodulation Combined with Fibrin Sealant and Bone Substitute Improving the Bone Repair of Critical Defects

et al. 2022

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In this preclinical protocol, an adjunct method is used in an attempt to overcome the limitations of conventional therapeutic approaches applied to bone repair of large bone defects filled with scaffolds. Thus, we evaluate the effects of photobiomodulation therapy (PBMT) on the bone repair process on defects filled with demineralized bovine bone (B) and fibrin sealant (T). The groups were BC (blood clot), BT (B + T), BCP (BC + PBMT), and BTP (B + T + PBMT). Microtomographically, BC and BCP presented a hypodense cavity with hyperdense regions adjacent to the border of the wound, with a slight increase at 42 days. BT and BTP presented discrete hyperdensing areas at the border and around the B particles. Quantitatively, BCP and BTP (16.96 ± 4.38; 17.37 ± 4.38) showed higher mean bone density volume in relation to BC and BT (14.42 ± 3.66; 13.44 ± 3.88). Histologically, BC and BCP presented deposition of immature bone at the periphery and at 42 days new bone tissue became lamellar with organized total collagen fibers. BT and BTP showed inflammatory infiltrate along the particles, but at 42 days, it was resolved, mainly in BTP. In the birefringence analysis, BT and BTP, the percentage of red birefringence increased (9.14% to 20.98% and 7.21% to 27.57%, respectively), but green birefringence was similar in relation to 14 days (3.3% to 3.5% and 3.5% to 4.2%, respectively). The number of osteocytes in the neoformed bone matrix proportionally reduced in all evaluated groups. Immunostaining of bone morphogenetic protein (BMP—2/4), osteocalcin (OCN), and vascular endothelial growth factor (VEGF) were higher in BCP and BTP when compared to the BC and BT groups (p < 0.05). An increased number of TRAP positive cells (tartrate resistant acid phosphatase) was observed in BT and BTP. We conclude that PBMT positively influenced the repair of bone defects filled with B and T.

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“…Similarly, Matheus et al, 18 evaluated the potential of different formulations of hyaluronic acid to improve the formation of new bone in critical-sized calvaria defects combined with a deproteinized bovine graft material (DBG), demonstrating that the combination of HA in a high viscosity crosslinking agent with DBG enhances the bone repair process and increases the amount of newly formed bone. These studies show, like the present study, that hyaluronic acid combined with another biomaterial improves the bone regeneration process.…”

Section: Discussionmentioning

confidence: 99%

Regenerating effect of hyaluronic acid on circumferential bone defects in Wistar albino rats.

Bueno-Beltrán¹,

Budiel-Salguero²,

Palacios-Bustamante³

et al. 2021

J Oral Res

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Objective: To determine the regenerating effect of hyaluronic acid on circumferential bone defects in albino Wistar rats. Material and Methods: An experimental type study was designed and carried out with 15 albino male Wistar rats, 4 months old and weighing between 250 and 350 grams. Two circumferential bone defects 3mm in diameter and 0.8mm deep were created in the calvaria of the parietal bone (on both sides of the midline). One defect was filled with a demineralized bone matrix (control group); while the other defect was filled with the combination of a demineralized bone matrix plus hyaluronic acid (experimental group). Five experimental rats were euthanized at 30, 60 and 90 days after surgery and they were histologically evaluated following the parameters proposed by Heiple. Results: The experimental group presented a better degree of bone regeneration at 30 and 60 postoperative days. Conclusion: Hyaluronic acid is effective in bone regeneration of circumferential bone defects.

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Association of hyaluronic acid with a deproteinized bovine graft improves bone repair and increases bone formation in critical‐size bone defects

Cited by 20 publications

References 51 publications

Application of Hyaluronic Acid as a Biopolymer Material in Reconstruction of Interdental Papilla in Rats

Application of Hyaluronic Acid as a Biopolymer Material in Reconstruction of Interdental Papilla in Rats

Use of Photobiomodulation Combined with Fibrin Sealant and Bone Substitute Improving the Bone Repair of Critical Defects

Regenerating effect of hyaluronic acid on circumferential bone defects in Wistar albino rats.

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