Association of Circulating Antibodies Against Double-Stranded and Single-Stranded DNA with Thyroid Autoantibodies in Graves' Disease and Hashimoto's Thyroiditis Patients

Abstract

The occurrence of antinuclear antibody (ANA), rheumatoid factor (RF), antibodies to double-stranded DNA (anti-dsDNA) and to single-stranded DNA (anti-ssDNA) was investigated in 51 patients with autoimmune thyroid diseases (AITD), and in 25 matched control subjects. In comparison with controls, the prevalence of anti-dsDNA was 74.5% in AITD patients (p=0.0001), 82.0% in 39 hyperthyroid Graves' disease (GD) (p=0.0001), and 50.0% in 12 euthyroid Hashimoto's thyroiditis (HT) patients (p=0.0001). The prevalence of anti-ssDNA was 90.1% in AITD (94.8% in GD and 75% in HT; p=0.001). The concentration of both anti-dsDNA and anti-ssDNA were higher (p=0.002) in AITD, in GD (p=0.001), and in HT (p=0.01) patients than in controls. Two patients with AITD were identified as positive for ANA. RF was detected in 4 AITD patients. Positive correlation was noted between anti-dsDNA with T4 (p=0.001), T3 (p=0.002), thyroid peroxidase antibody (anti-TPO) (p=0.0001), and TSH (p=0.001) values but not with thyroglobulin antibody (anti-Tg). Serum anti-ssDNA values were also correlated with T3 (p=0.0001), TSH (p=0.003), and anti-TPO (p=0.0001). However, by using a multiple regression analysis only anti-TPO remained associated with anti-dsDNA and both anti-Tg and anti-TPO with anti-ssDNA values. The predisposition to develop systemic autoimmune disorders is not influenced by thyroid hormones. The elevated prevalence of serum anti-dsDNA and anti-ssDNA in AITD patients points out that we must be aware of the risk for predisposition for the development of other systemic autoimmune diseases.

Acute hepatitis E virus infection and autoimmune thyroiditis: yet another trigger?

“…In fact, components of several viruses have been found in the thyroid gland of patients with autoimmune thyroiditis and activation of auto-reactive T cells as well as collateral damage of other antiviral responses might be involved in the pathogenesis of autoimmune thyroiditis 7. On the other hand, a variety of auto-antibodies including antinuclear antibodies – as were present in our patient – have been described for autoimmune thyroiditis8 9 but also in patients with asthma bronchiale 10…”

Biochemical and immunological markers of autoimmune thyroiditis

“…Встречаемость АТ-дДНК составила 90% от общей выборки (ХЛТ и БГ), -из них -75% у больных ХЛТ [20]. Результаты наших исследований сыворотки крови больных ХЛТ также показали преобладание пула АТ-дДНК.…”

“…В работе Pedro и соавт. [20] также показано отсутствие корреляционной зависимости между АТ-нДНК и АТ-ТГ у больных эутиреозом. При этом активность гидролизующих АТ обнаруживает корреляционную зависимость с тяжестью клинической картины ХЛТ, а также с явлениями дисфункции и разрушения ЩЖ [33], что имело место в нашем исследовании.…”

Algorithmic Approach With Clinical Pathology Consultation Improves Access to Specialty Care for Patients With Systemic Lupus Erythematosus

“…Patients with thyroid disease and hepatitis may have symptoms that overlap with collagen vascular disease and positive ANAs. [8][9][10][11] Thus, screening for thyroid disease with thyroid-stimulating hormone (TSH) and a hepatitis panel that includes hepatitis C virus immunoglobulin G, hepatitis B virus immunoglobulin M (IgM) to core antigen and surface antigen, and hepatitis A virus IgM is performed.…”