Association between the MTHFR C677T polymorphism, blood folate and vitamin B12 deficiency, and elevated serum total homocysteine in healthy individuals in Yunnan Province, China

Ni, Juan; Zhang, Ling; Zhou, Tao; Xu, Weijiang; Xue, Jinglun; Neng, Cao; Wang, Xu

doi:10.1016/j.jcma.2016.07.005

Cited by 56 publications

(40 citation statements)

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“…In addition, the subjects with TT genotype had significantly higher tHcy levels than those CC genotype (5.44 ± 1.32 μmol/L vs. 4.67 ± 1.07 μmol/L, respectively; P=0.010). The results were consistent with the previous studies [24,43,44]. Because the prevalence (19%) of 677TT genotype is high in China, the risk of exposure to plasma folate deficiency, resultant hyperhomocysteinemia, and adverse pregnancy outcome may elevate [45][46][47].…”

Section: Discussionsupporting

confidence: 92%

Association between Decreased Plasma Folate Levels and MTHFR C677T, and MTRR A66G Gene Polymorphisms as Determinants for Elevated Total Homocysteine Concentrations in Pregnant Women

Song¹,

Song²,

Bao³

et al. 2018

Hereditary Genet Curr Res

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Aims: The aims of this paper were to examine the association between gene polymorphisms of methylenetetrahydrofolate reductase (MTHFR) C677T and methionine synthase reductase (MTRR) A66G with levels of plasma folate and tHcy. Methods:Peripheral venous blood samples were obtained from 143 pregnant women, who had been fasted for more than 10 hours. Plasma tHcy and folate concentrations were measured in plasma specimens. The genotypes for polymorphisms were identified by the PCR-restriction fragment length polymorphism (RFLP). Results:Our results demonstrated that MTHFR 677T allele was the predictors of plasma folate levels in pregnant women. While MTHFR 677TT genotype and MTRR 66G allele were the predictors of plasma tHcy concentrations. Plasma folate levels were negatively correlated with tHcy concentrations (Pearson's correlation coefficient r=-0.358, p=0.000012). With the increase of gestational weeks, the levels of plasma folate was first risen and then fall, whereas, there was a downtrend between gestational weeks and tHcy levels. Conclusion:Plasma folate status and total homocysteine level associated with MTHFR C677T and MTRR A66G gene polymorphisms in pregnant women. According to our study, the pregnant women of homozygous mutation of MTHFR 677TT and/or MTRR 66GG genes generally have lower levels of folate. Therefore, we recommend that pregnant women with 677TT and 66GG genotypes should supplement the appropriate dosage of folic acid based on their genotypes.

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Section: Discussionsupporting

confidence: 92%

Association between Decreased Plasma Folate Levels and MTHFR C677T, and MTRR A66G Gene Polymorphisms as Determinants for Elevated Total Homocysteine Concentrations in Pregnant Women

Song¹,

Song²,

Bao³

et al. 2018

Hereditary Genet Curr Res

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“…The increase in the frequency of pathological alleles of the folic acid cycle genes in women with disorders of the pregnancy course, as compared to the control group, revealed in the present study is supported by numerous literature data, for example [9]. However, the lack of direct correlation between the genotype of the four most clinically significant SNPs and the blood homocysteine level detected is in some contradiction with the literature data, for example [10]. This phenomenon can be pretty explained, on the one hand, by the features of the sample being analyzed and on the other hand, by the difference in the mean square deviation of the homocysteine level fluctuation over time in specific patients.…”

Section: Discussionsupporting

confidence: 70%

The Fluctuations in Homocysteine Level Caused by Various Combinations of Folic Acid Cycle Genes Snp Alleles as a Factor in the Course of Pregnancy Violation

Ev¹,

Yn²,

Ma³

et al. 2018

Adv Tech Biol Med

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The presence of pathological alleles of single nucleotide polymorphisms (SNP) of the folic acid cycle genes is one of the female reproductive system violation factors including habitual miscarriage and pre-eclampsia. The realizing mechanism for this genetic predisposition is hyperhomocysteinemia-homocysteine level increasing in the blood. This study presenting an attempt to find the relationship between the genotype for the four SNPs of the three folate cycle genes-C677T and A1298C of the MTHFR gene, A2756G of the MTR gene and the A66G of the MTRR gene and the homocysteine level in the blood of women with impaired pregnancy. As a result no direct correlation was found but it was found a statistically significant interlation between the presence of pathological alleles of the studied SNP and the mean square deviation (σ) of the homocysteine level fluctuations over time. For the polymorphism C677T of the MTHFR gene σ of the homocysteine blood level fluctuation is increased up to four times in women with a homozygous pathological state TT compared with the normal homozygotes CC. The clinical importance of monitoring the homocysteine blood level has been shown especially for women with folate cycle genes pathological alleles' presence.

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“…Elevated homocysteine level can be either due to deficiency of folate or Vitamin B 12 [20]. A recent study carried out in China among 330 study volunteers also reported folate and Vitamin B 12 deficiency as well as MTHFR C677T polymorphism were significantly associated with elevated serum homocysteine levels [21]. Higher prevalence of hyperhomocysteinemia has been reported among vegetarians mainly due to Vitamin B 12 deficiency [22].…”

Section: Research Articlementioning

confidence: 99%

Homocysteine Status of Elderly Slum Women in Delhi

Gonmei

Dwivedi

Siddhu

et al. 2018

Asian J Pharm Clin Res

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Objective: The study was undertaken to assess homocysteine levels among economically deprived elderly women in Delhi.Methods: The study was carried out among elderly women aged 60–70 years residing in Kirti Nagar slums of West Delhi. Blood samples were collected, and serum homocysteine was analyzed using chemiluminescent immunometric assay. Hyperhomocysteinemia was defined as serum homocysteine >15 μmol/l. Dietary information was also collected using 24 h dietary recall method and food frequency questionnaire.Results: The prevalence of hyperhomocysteinemia was 16.9%. The mean serum homocysteine was 12.35±6.43 μmol/l. Serum homocysteine levels ranged from 3.84 to 35.20 μmol/l. It was observed that hyperhomocysteinemia was higher in vegetarians (31.2%) compared to non-vegetarians (6.7%).Conclusion: The prevalence of hyperhomocysteinemia is higher among vegetarians than non-vegetarians. Further, research is necessary to see the effect of elevated homocysteine in multiple age-related diseases.

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Association between the MTHFR C677T polymorphism, blood folate and vitamin B12 deficiency, and elevated serum total homocysteine in healthy individuals in Yunnan Province, China

Cited by 56 publications

References 56 publications

Association between Decreased Plasma Folate Levels and MTHFR C677T, and MTRR A66G Gene Polymorphisms as Determinants for Elevated Total Homocysteine Concentrations in Pregnant Women

Association between Decreased Plasma Folate Levels and MTHFR C677T, and MTRR A66G Gene Polymorphisms as Determinants for Elevated Total Homocysteine Concentrations in Pregnant Women

The Fluctuations in Homocysteine Level Caused by Various Combinations of Folic Acid Cycle Genes Snp Alleles as a Factor in the Course of Pregnancy Violation

Homocysteine Status of Elderly Slum Women in Delhi

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