Association Between Serum Aminotransferase enzymes–lipid Profile Ratio and Spontaneous HCV Clearance in Blood Donors

Tabll, Ashraf A.; Kodous, Marwa A; Abbas, Ayman T.; Omran, Mohamed M.; Elsayed, Elsherbeny H.

doi:10.2217/fvl.15.103

Cited by 2 publications

(1 citation statement)

References 47 publications

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“…It was reported that the mean HAI and fibrosis scores of the PCR negative women were also significantly lower than those of PCR positive women (p=0.000). 23 Also, the present study showed that CHC patients had significantly lower PLC than SVC subjects. This could be explained by the presence of hypersplenism, decreased platelet production due to decreased hepatic thrombopoietin (TPO) secretion and CHC induced bone marrow suppression.…”

Section: Discussionsupporting

confidence: 63%

Single nucleotide polymorphism of the promotor region of Interleukin-10 gene did not show significant association with spontaneous clearance of hepatitis C virus infection in Egyptian patients

Waly,

Zaki,

Shabana

et al. 2023

GHOA

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Introduction: Accurate etiologies of spontaneous clearance of HCV infection are hard to be clarified. However, it is proposed that genetically determined interleukin-10 (IL-10) changes play an important function in HCV elimination. Individuals who are homozygous for IL-10 gene promoter region 1082 (G/A; rs1800896) genotype AA have significantly lower levels of plasma IL-10 levels with better capability of spontaneous viral clearance (SVC); while those with GG genotype have two folds greater level of IL-10 than GA or AA persons, leading to diminished capability of SVC. Aims: The study aimed at identifying the IL-10 gene promoter region 1082 (G/A; rs1800896) polymorphism as a possible predictor of SVC of HCV infection in Egyptian patients and its relation with the viral load and the degree of liver injury in chronic hepatitis C (CHC) patients. Methods: The study was conducted on two groups; group I which included 100 cases of CHC defined as detectable anti-HCV antibodies and detectable serum HCV RNA for ≥ 6 months and group II which included 50 cases of SVC defined as detectable anti-HCV antibodies for ≥ 6 months and undetectable HCV RNA in two consecutive tests, 3 months apart, without previous antiviral therapies. HCV antibody testing was done using 4th generation enzyme-linked immuno sorbent assay (ELISA) technique. HCV-RNA quantification was done using polymerase chain reaction (PCR) technique. Detection of IL10 1082 (G/A; rs1800896) polymorphism was done by PCR restriction fragment length polymorphism (RFLP) technique. Complete blood count (CBC); serum albumin, serum bilirubin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), international normalized ratio (INR), serum creatinine and HBsAg, were done for all participants. Child-Turcotte-Pugh (CTP) class & FIB4 score were calculated. Abdominal ultra-sonography (US) and contrast enhanced computed tomography (CT) of the abdomen were done for all participants. Results: In group I, the age was 53.6±11.2 years old, males represented 51% and females represented 49%. In group II, the age was 52.8±8.6 years old, males represented 48% and females represented 52%. No significant differences were found regarding age and gender (p=0.658, 0.729 respectively). Group I showed significantly higher frequency of hepatomegaly, splenomegaly & liver cirrhosis (p < 0.001) and significantly lower platelet count (PLC) (183.5±61.1 versus 220.3±67.2 ×109/L, p=0.006). Group I showed significantly higher ALT, AST & FIB4 score (p <0.001, 0.002, <0.001 respectively). Group I had median viral load of 555640 IU/mL. Applying Hardy Weinberg (HW) equation, IL10 1082 (G/A; rs1800896) genotypes in both groups were in HW equilibrium (HW p=0.845&0.861 respectively). In Group I, genotype AA represented 72%, AG represented 26% & GG represented 2%, while in Group II, genotype AA represented 62%, AG represented 34% & GG represented 4% ,with insignificant differences between both groups (p=0.272, 0.415 & 0.216 respectively). In Group I, no significant differences regarding HCV RNA load ,CTP class, PLC , FIB4 score ,were found between the three genotypes (p=0.191, 0.086, 0.754,0.829, 0.187 respectively). Multivariate logistic regression analysis showed that IL10 1082 (G/A; rs1800896) genotypes was not a significant predictor of SVC of HCV infection in Egyptian patients (p=0.216.OR=1.322, 95% CI=0.849-2.058). Conclusion: Single nucleotide polymorphism of the promoter region of the IL-10 gene, 1082 (G/A; rs1800896), did not show significant association with the spontaneous clearance of hepatitis C virus infection in Egyptian patients. In chronic hepatitis C patients, this SNP had no significant association with the serum HCV viral load, CTP class, PLC or FIB4 score.

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Section: Discussionsupporting

confidence: 63%

Single nucleotide polymorphism of the promotor region of Interleukin-10 gene did not show significant association with spontaneous clearance of hepatitis C virus infection in Egyptian patients

Waly,

Zaki,

Shabana

et al. 2023

GHOA

View full text Add to dashboard Cite

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Peripheral Expression ofCXCL10Gene in Chronic Hepatitis C Patients Treated with Sofosbuvir, Daclatasvir, and Ribavirin

Afzal

Ali

Sheikh

et al. 2020

Journal of Interferon & Cytokine Research

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Hepatitis C virus (HCV) causes persistent infection and invades host's innate and adaptive immune systems. During the eradication of this pathogen, the components of immune system may cause bystander damage to host, which might be even worse than the viral pathogenesis. Thus, the therapy should not only eliminate primary virus infection but also improve the inflammatory immune responses. The breakthrough of interferon free direct acting antiviral (DAA) drugs has provided the opportunity to unravel the association of HCV with immune response. This study aimed to examine the expression level of C-X-C motif chemokine ligand 10 (CXCL10) in the Peripheral blood mononuclear cells (PBMCs) of HCV infected patients treated with DAAs + Ribavirin. In this study we analyzed the expression levels of CXCL10 mRNA in the 90 chronic HCV patients using quantitative PCR (qPCR) prior, after, and during therapy with sofosbuvir/ribavirin (SOF+RBV) and sofosbuvir/daclatasvir/ribavirin (SOF+DCV+RBV), and further, the results were analyzed relative to treatment response. Significantly elevated CXCL10 mRNA was seen in naive patients having higher viral load (P = 0.005) and those suffering from hepatocellular carcinoma (P = 0.006). HCV patients had remarkable decline in CXCL10 level after 4, 12, and 24 weeks of therapy with DAAs. An approximate one-fold decrease was observed in patients who attained sustained virological response compared to untreated patients (P < 0.0001). Comparing the 2 regimens, the reduction in peripheral CXCL10 expression was more pronounced in patients undergoing SOF+DCV+RBV therapy. The current study implicitly shows the role of CXCL10 as an indicator of disruption of host-virus equilibrium and consequent pathogenesis of HCV during successful antiviral therapy. Furthermore, the drop in CXCL10 level after HCV viral clearance might reflect the DAA-induced alleviation in the extrahepatic manifestation of this infection.

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Association Between Serum Aminotransferase enzymes–lipid Profile Ratio and Spontaneous HCV Clearance in Blood Donors

Cited by 2 publications

References 47 publications

Single nucleotide polymorphism of the promotor region of Interleukin-10 gene did not show significant association with spontaneous clearance of hepatitis C virus infection in Egyptian patients

Single nucleotide polymorphism of the promotor region of Interleukin-10 gene did not show significant association with spontaneous clearance of hepatitis C virus infection in Egyptian patients

Peripheral Expression ofCXCL10Gene in Chronic Hepatitis C Patients Treated with Sofosbuvir, Daclatasvir, and Ribavirin

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