Association between interleukin 4 (IL-4) VNTR, gene polymorphism, and breast cancer susceptibility in Iranian population: experimental and web base analysis

Ibrahimi, Mostafa; Jamalzei, B.; Akbari, M. E.; Ibrahimi, Reza; Alaei, Mohammad Reza; Moossavi, Maryam; Mohammadoo‐Khorasani, Milad

doi:10.4149/bll_2018_116

Cited by 11 publications

(8 citation statements)

References 36 publications

(38 reference statements)

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“…Although cachexia is generally observed in pancreatic, gastric, colorectal, lung, and head and neck cancer, recent studies have provided a compelling evidence that there is a relationship between advance metastatic breast cancer and cachexia due to a decrease in muscle mass and excessive weight loss (53)(54)(55). Recent studies have also demonstrated that a high level of IL-6 is associated with cachexia, angiogenesis, atrophy, increased catabolism of muscle protein and resistance to treatment in advance breast cancer (2,35,56).…”

Section: Discussionmentioning

confidence: 99%

IL-6 mediated JAK/STAT3 signaling pathway in cancer patients with cachexia

Eskiler¹,

Bezdegümeli²,

Özman³

et al. 2019

BLL

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OBJECTIVES: We investigated the changes in the IL-6 and STAT3 expression levels in cachectic and noncachectic patients with gastric, lung and breast cancer and evaluated the association between IL-6 and STAT3 levels and cancer types in terms of cachexia condition. BACKGROUND: Cancer-associated cachexia, observed in nearly 50-80 % of cancer patients, has drawn attention in advanced patients. IL-6/JAK/STAT pathway plays an essential role in the progression of cancer cachexia through the regulation of the infl ammatory response. METHODS: This study consisted of 48 gastric, breast and lung cancer patients (18 cachectic and 30 noncachectic) and healthy individuals. Total RNA isolation and cDNA synthesis was performed after the collection of blood samples. IL-6 and STAT3 expression levels were analyzed by RT-PCR analysis. RESULTS: Our fi ndings demonstrated that IL-6 mRNA levels considerably increased 19.89 ± 8.25, 5.18 ± 2.81 and 15.33±9.54-fold in gastric, lung and breast cancer patients with cachexia, respectively. Additionally, a 16.67±7.13, 14.21 ± 11.72 and 8.85 ± 3.89-fold increase in the STAT3 expression level was detected in cachectic gastric, lung and breast cancer patients, respectively (p < 0.01). CONCLUSION: STAT3 may be considered as a therapeutic target for cachectic patients with gastric, lung and breast cancer. Furthermore, IL-6 mediates STAT3 activation in cachectic gastric and breast cancer patients (Tab. 5, Fig. 2, Ref. 62).

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Section: Discussionmentioning

confidence: 99%

IL-6 mediated JAK/STAT3 signaling pathway in cancer patients with cachexia

Eskiler¹,

Bezdegümeli²,

Özman³

et al. 2019

BLL

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“…2R/2R carrier genotypes (3R/2R and 2R/2R) were reported to be associated with late stage bladder cancer in northern Indian population (28) and 2R/2R genotype refl ected a signifi cant association for bladder cancer and tumor invasiveness in a Taiwanese population (29). Concertedly with previous studies, 3R/3R genotype of IL-4 VNTR was proven as a protective factor against breast cancer susceptibility in an Iranian population (30) while the effect of IL-4 VNTR variants was not observed in Jordanian breast cancer patients (31). In adult acute myeloid leukemia patients, the frequency of 3R alleles was higher than in controls and 3R/3R genotype was reported to be signifi cantly associated with poor therapeutic response, higher susceptibility to disease recurrence and shorter overall survival and disease-free survival (32).…”

Section: U N C O R R E C T E Dmentioning

confidence: 65%

Predictive value of KRAS/NRAS, IL-4 VNTR and HPV alterations in metastases of colorectal cancer

OZEN¹,

YEGIN²,

AVSAR³

et al. 2022

BLL

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PURPOSE:The main aim of the study was to evaluate the potential roles of KRAS/NRAS proto-oncogenes, IL-4 VNTR variants and HPV prevalence in colorectal cancer metastasis. As the second aim, the interactions of the analyzed genes and viral sequences with both clinicopathological variables and each other were targeted. METHODS: DNA was extracted using AmoyDx FFPE DNA Extraction kit from paraffi n-embedded colorectal tumor tissue samples (n = 60). NRAS/KRAS mutational profi les were determined with real-time polymerase chain reaction using AmoyDx KRAS/NRAS Mutation Detection Kit. Genotyping of IL-4 VNTR was made with PCR. HPV detection was analyzed by PCR with both GP5+/GP6+ consensus primers and type-specifi c primers for HPV-16 and HPV-18. SPSS v22 (IBM) statistics software was used for all statistical analyses. RESULTS: From the demographical/clinicopathological parameters, age and biopsy specimens revealed an association with metastasis. KRAS mutation rate was as high as 65 % in the patients and the most prevalent mutation type was G12D. Metastasis risk was 3.19-fold increased in KRAS-mutated patients compared to KRAS-negative ones. IL-4 VNTR genotypes/alleles were not associated with metastasis in our analysis. The frequency of HPVs in our colorectal cancer cohort was 36.7 %, but HPV positivity was not found to be associated with metastasis. A signifi cant association was found between HPV and NRAS mutations; NRAS wild-type status acted as a protective factor by 7.5-fold for HPV negativity. CONCLUSION: Our study comprehensively and concomitantly evaluated several potential molecular risk factors. Future studies designed in such combined approaches will substantially contribute to better manage colorectal cancer tumorigenesis from molecular biological perspective (Tab. 6, Fig. 2, Ref. 40).

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“…In a recent study, Ibrahimi et al suggested that RP2\RP2 genotypes might be a potent protective factor against BC 39. In addition, Jia et al40 examined in a comprehensive meta-analysis the correlation between different types of genetic variants within the IL-4 gene, including the VNTR, and cancer risk.…”

Section: Discussionmentioning

confidence: 99%

<p>The influence of an<em> IL-4</em> variable number tandem repeat (VNTR) polymorphism on breast cancer susceptibility</p>

AL-Eitan¹,

Rababa'h²,

Alghamdi³

et al. 2019

PGPM

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BackgroundsBreast cancer (BC) is one of the most widespread cancers globally. Understanding the etiology of BC may help in determining the various risk factors involved in its malignancy. Certain genetic mutations are considered to play a key role in increasing the risk of BC.ObjectivesIn this study, we explored the correlation between a variable number tandem repeat (VNTR) polymorphism in the IL-4 gene and BC.MethodsPCR and subsequent gel electrophoresis were used to genotype this variant in 360 Jordanian women (180 BC patients and 180 controls). In addition, phenotype–genotype analysis was carried out.ResultsOur findings illustrate that there is no significant relationship between the variant genotypes in the IL-4 gene and BC among Jordanian females. Other than body mass index and tumor differentiation (p< 0.05), none of the clinical and pathological parameters of BC patients exhibited any association with the variant genotypes.ConclusionsFrom this study, we propose that the IL-4 genetic variant does not impact BC development and progression but that it could influence the disease prognosis.

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Association between interleukin 4 (IL-4) VNTR, gene polymorphism, and breast cancer susceptibility in Iranian population: experimental and web base analysis

Cited by 11 publications

References 36 publications

IL-6 mediated JAK/STAT3 signaling pathway in cancer patients with cachexia

IL-6 mediated JAK/STAT3 signaling pathway in cancer patients with cachexia

Predictive value of KRAS/NRAS, IL-4 VNTR and HPV alterations in metastases of colorectal cancer

<p>The influence of an<em> IL-4</em> variable number tandem repeat (VNTR) polymorphism on breast cancer susceptibility</p>

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