Association between Epstein - Barr Virus (EBV) and Breast Cancer

Torabizadeh, Zhila; Nadji, Alireza; Naghshvar, Farshad; Nosrati, Anahita; Parsa, Mohsen

doi:10.18869/acadpub.rmm.2.4.24

Cited by 7 publications

(5 citation statements)

References 38 publications

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“…For EBV, the screening of the viral genome did not show any correlation with the case and control groups (P = 0.164) ( Table 3). The rate of EBV infection in breast cancer patients was not unexpected, as the virus has been detected in many studies to be absent or be at a low rate at this site (45)(46)(47). However, we did not observe any significant association between breast cancer and EBV presence.…”

Section: Discussioncontrasting

confidence: 49%

Downregulation of miR-143/145 Cluster in Breast Carcinoma Specimens: Putative Role of DNA Oncoviruses

Nakhaie

Makvandi

Charostad

et al. 2021

Jundishapur J Microbiol

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Background: The DNA oncoviruses, including human papillomavirus (HPV) and Epstein-Barr virus (EBV) are among the most important infectious agents involved in breast carcinogenesis. These oncoviruses have a broad disrupting effect on cellular miRNAs and their functions, by which they contribute to carcinogenesis. Objectives: In this investigation, we evaluated the correlation between HPV and EBV and the expression level of tumor suppressor miRNAs (miR-143 and 145), clinical outcomes, and their association with stimulating inflammatory cytokines in patients with breast carcinoma. Methods: In our case-control study, 35 cancerous tissues and 35 adjacent non-cancerous tissues were collected from 35 patients. Nested-PCR was set up for the detection of HPV and EBV genomes, and RT-qPCR was used for miRNA expression in the case and control groups. In addition, serum specimens were obtained from all patients (n = 35) and healthy controls (n = 35) to determine the IL-8 serum concentration. Results: We found HPV and EBV in 14.2% (10/70) and 7% (5/70) of all samples, respectively. The distributions of positive samples in the case and control groups were 25.7% (9/35) and 2.9% (1/35) (P = 0.006) for HPV and 11.4% (4/35) and 2.9% (1/35) (P = 0.164) for EBV, respectively. Besides, RT-qPCR showed that miR-143 and miR-145 were significantly downregulated in HPV and EBV-infected cases compared to non-infected ones (P < 0.05). Data also indicated that the promotion of metastasis status was related to miR-143/145 downregulation and HPV infection (P = 0.003). No significant difference was found in serum IL-8 concentration concerning viral infections. Conclusions: Our results suggested the possible involvement of viral infections in breast carcinogenesis and adverse clinical outcomes by downregulating miR-143/145.

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Section: Discussioncontrasting

confidence: 49%

Downregulation of miR-143/145 Cluster in Breast Carcinoma Specimens: Putative Role of DNA Oncoviruses

Nakhaie

Makvandi

Charostad

et al. 2021

Jundishapur J Microbiol

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“…The low frequency of 7.3% EBV DNA have been reported among the breast cancer in Iran (Torabizadeh et al, 2014). While high frequency of 55.5% of EBV DNA detection in women with breast cancer have been reported in Sudan (Yahia et al, 2014).…”

Section: Discussionmentioning

confidence: 94%

Frequency of Epstein–Barr Virus DNA in Formalin-fixed Paraffin-embedded Tissue of Patients with Ductal Breast Carcinoma

Sharifpour

Makvandi

Samabafzadeh

et al. 2019

Asian Pac J Cancer Prev

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Background:Ductal carcinoma is one of the most common breast cancer (BrC) among the women in the world. Several factors may involve in establishment of breast cancer. The role of viral infections have been investigated in BrC, Among them the association of Epstein Barr virus have been reported in the patients with breast cancer type ductal carcinoma. Thus this study was conducted to evaluate the rate of Epstein Barr virus in women with breast cancer type ductal carcinoma.Material and methods:A total of 72 formalin-fixed paraffin-embedded tissue blocks samples were collected from 37 (51.38%) women with breast cancer type ductal carcinoma and 35 (48.61%) samples of breast with fibro adenoma as control group. The DNA was extracted for all the samples. The detection of EBNA 3C EBV DNA was done by nested PCR. The results of positive were sequenced to confirm PCR product and determine EBV genotypes.Results:About 10/37 (27.02%) samples of ductal breast carcinoma were showed positive for EBNA 3C EBV DNA while 4/35 (11.42%) of fibro adenoma were positive for EBNA 3C EBV DNA (p= 0.095). Randomly 7 PCR products were sequenced and the results of sequencing EBNA 3C shows, the detected EBVDNA were type 1 EBV type.Conclusion:This study shows high prevalence of 27.02% EBV DNA type 1 was found in formalin-fixed paraffin-embedded tissue of Patients with ductal breast carcinoma. The outcomes of this study suggesting that EBV might have a significant role in breast cancer in Ahvaz city, south west region of Iran. However the expression of EBV oncoproteins, EBNA1, LMP1, and LMP2 require to be determined with ductal carcinoma cells. About 72.97% breast samples showed negative for EBVDNA. The role other viruses including Human cytomegalovirus, papilloma viruses and Merkel viruses are required to be investigated in further studies.

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“…In addition, Eghbali et al [16] in Tehran, Iran, reported EBV infection in 16.6% of carcinoma and 4.1% of fibroadenoma samples, but this infection rate was statistically insignificant. A low frequency (7.3%) of EBV DNA have also been reported among the breast cancer in another study in Iran [17] .…”

Section: Discussionmentioning

confidence: 65%

Molecular Detection of Epstein-Barr Virus and Human Cytomegalovirus Antigen Expression in Breast Cancer in Khartoum State, Sudan 2018

Aidrous

Hussein²,

Dahawi

et al. 2019

NACS

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Background: Breast cancer is a leading cause of death among women worldwide. The association between Epstein-Barr Virus (EBV) and Human Cytomegalovirus (HCMV) and breast cancer risk still remains controversial making it difficult to determine whether either, both or neither virus is causally associated with breast cancer. The aim of this study was to detect EBV and the expression of the Immediate Early Antigen (IE) of HCMV in breast cancer in Sudanese women.Method: 60 Formalin-fixed and paraffin-embedded tissue blocks from 42 ductal breast carcinoma and18 benign breast tumors (control group) were obtained from the pathology laboratories of several hospitals in Khartoum State, Sudan, and used in this study. We used PCR to detect EBV and the IE-antigen of HCMV in ductal breast carcinoma and benign breast tumor.Result: Only 3 (7%) out of 42 ductal breast carcinoma were positive for EBV DNA and none of 18 benign breast tumor (control group) were positive for EBV DNA and all 60 specimens were negative for IE-antigen of HCMV expression. Conclusion:The results of this study failed to show any relationship between EBV and HCMV and the development of breast carcinoma in the tested specimens.

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Association between Epstein - Barr Virus (EBV) and Breast Cancer

Cited by 7 publications

References 38 publications

Downregulation of miR-143/145 Cluster in Breast Carcinoma Specimens: Putative Role of DNA Oncoviruses

Downregulation of miR-143/145 Cluster in Breast Carcinoma Specimens: Putative Role of DNA Oncoviruses

Frequency of Epstein–Barr Virus DNA in Formalin-fixed Paraffin-embedded Tissue of Patients with Ductal Breast Carcinoma

Molecular Detection of Epstein-Barr Virus and Human Cytomegalovirus Antigen Expression in Breast Cancer in Khartoum State, Sudan 2018

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