Assignment of CDC Weak Oxidizer Group 2 (WO-2) to the Genus
            <i>Pandoraea</i>
            and Characterization of Three New
            <i>Pandoraea</i>
            Genomospecies

Daneshvar, Maryam I.; Hollis, D G; Steigerwalt, Arnold G.; Whitney, Anne M.; Spangler, Laura; Douglas, Michael P.; Jordan, Jean G.; MacGregor, John P.; Hill, Bertha C.; Tenover, Fred C.; Brenner, Don J.; Weyant, Robbin S.

doi:10.1128/jcm.39.5.1819-1826.2001

Cited by 74 publications

(110 citation statements)

References 20 publications

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“…AF247693), Pandoraea genomospecies 3 (AF247697), and a single isolate of Pandoraea apista (AF247699, but none of the other P. apista isolates in GenBank). However, DNA-DNA hybridization studies have previously established that these four isolates, with similar 16S rRNA gene sequences, are in fact located in different species-level hybridization groups (9). After a review of our records and those provided by the external laboratory, the biochemical profile of our isolates was found to be most consistent with characteristics reported for P. sputorum isolates described in previous studies (6,9): no growth at 42°C, growth on cetrimide agar, negative nitrate reduction, positive urease activity, and a negative oxidase reaction.…”

Section: Case Reportmentioning

confidence: 99%

“…However, DNA-DNA hybridization studies have previously established that these four isolates, with similar 16S rRNA gene sequences, are in fact located in different species-level hybridization groups (9). After a review of our records and those provided by the external laboratory, the biochemical profile of our isolates was found to be most consistent with characteristics reported for P. sputorum isolates described in previous studies (6,9): no growth at 42°C, growth on cetrimide agar, negative nitrate reduction, positive urease activity, and a negative oxidase reaction. While these studies have demonstrated that the phenotypic characteristics may vary between isolates of the same species, absence of growth at 42°C appears to be a characteristic only of P. sputorum, Pandoraea norimbergensis, and Pandoraea genomospecies 1 (the last two of which were not in question, due to other differing characteristics).…”

Section: Case Reportmentioning

confidence: 99%

See 1 more Smart Citation

Misidentification of Pandoraea sputorum Isolated from Sputum of a Patient with Cystic Fibrosis and Review of Pandoraea Species Infections in Transplant Patients

Pimentel

MacLeod

2008

J Clin Microbiol

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Pandoraea species are considered emerging pathogens in cystic fibrosis (CF) patients, but few data exist regarding outcomes of patients colonized with these organisms. We report a case of Pandoraea sputorum colonization in a CF patient under consideration for lung transplantation and review five cases of lung transplantation involving Pandoraea species.

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Section: Case Reportmentioning

confidence: 99%

Section: Case Reportmentioning

confidence: 99%

Misidentification of Pandoraea sputorum Isolated from Sputum of a Patient with Cystic Fibrosis and Review of Pandoraea Species Infections in Transplant Patients

Pimentel

MacLeod

2008

J Clin Microbiol

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“…Pandoraea colonization has been associated with CF and non-CF patients and often occurs when the patient has already succumbed to chronic colonization with another pathogen (Schneider et al, 2006). Nine species have been identified within the genus to date Daneshvar et al, 2001;Sahin et al, 2011). Pandoraea apista has been associated with epidemic spread and rapid patient decline (Atkinson et al, 2006;Jørgensen et al, 2003), while Pandoraea pulmonicola is the most commonly identified Pandoraea species among Irish CF patients (Costello et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of co-colonizing cystic fibrosis-associated pathogens by Pseudomonas aeruginosa and Burkholderia multivorans

et al. 2014

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Cystic fibrosis (CF) is a recessive genetic disease characterized by chronic respiratory infections and inflammation causing permanent lung damage. Recurrent infections are caused by Gramnegative antibiotic-resistant bacterial pathogens such as Pseudomonas aeruginosa, Burkholderia cepacia complex (Bcc) and the emerging pathogen genus Pandoraea. In this study, the interactions between co-colonizing CF pathogens were investigated. Both Pandoraea and Bcc elicited potent pro-inflammatory responses that were significantly greater than Ps. aeruginosa. The original aim was to examine whether combinations of pro-inflammatory pathogens would further exacerbate inflammation. In contrast, when these pathogens were colonized in the presence of Ps. aeruginosa the pro-inflammatory response was significantly decreased. Real-time PCR quantification of bacterial DNA from mixed cultures indicated that Ps. aeruginosa significantly inhibited the growth of Burkholderia multivorans, Burkholderia cenocepacia, Pandoraea pulmonicola and Pandoraea apista, which may be a factor in its dominance as a colonizer of CF patients. Ps. aeruginosa cell-free supernatant also suppressed growth of these pathogens, indicating that inhibition was innate rather than a response to the presence of a competitor. Screening of a Ps. aeruginosa mutant library highlighted a role for quorum sensing and pyoverdine biosynthesis genes in the inhibition of B. cenocepacia. Pyoverdine was confirmed to contribute to the inhibition of B. cenocepacia strain J2315. B. multivorans was the only species that could significantly inhibit Ps. aeruginosa growth. B. multivorans also inhibited B. cenocepacia and Pa. apista. In conclusion, both Ps. aeruginosa and B. multivorans are capable of suppressing growth and virulence of co-colonizing CF pathogens.

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“…However, Coenye et al (2000) reported the absence of assimilation of substrates such as L-arabinose and Dmannitol and the presence of alkaline phosphatase activity in Pandoraea species, for which the present study observed discrepancies. Such laboratory-to-laboratory discrepancies show the importance of obtaining and testing valid reference strains to verify their reactions with in-house procedures (Daneshvar et al, 2001). Strain ATSB16 T can be differentiated from other Pandoraea species on the basis of its ability to assimilate D-mannose, maltose, L-rhamnose and L-fucose and inability to assimilate capric acid.…”

mentioning

confidence: 99%

“…Currently, the genus Pandoraea consists of five species, namely Pandoraea pnomenusa, P. pulmonicola, P. apista, P. norimbergensis and P. sputorum, and four genomospecies (Coenye et al, 2000;Daneshvar et al, 2001). During a study of thiosulfate-oxidizing bacterial populations in sesame rhizosphere soils, one of the isolates, strain ATSB16 T , was subjected to phenotypic, genotypic and chemotaxonomic investigations in order to determine its taxonomic position.…”

mentioning

confidence: 99%

Pandoraea thiooxydans sp. nov., a facultatively chemolithotrophic, thiosulfate-oxidizing bacterium isolated from rhizosphere soils of sesame (Sesamum indicum L.)

Anandham

Indiragandhi

Kwon

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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A facultatively chemolithoautotrophic, thiosulfate-oxidizing, Gram-negative, aerobic, motile, rodshaped bacterial strain, designated ATSB16 T , was isolated from rhizosphere soils of sesame (Sesamum indicum L.). 16S rRNA gene sequence analysis demonstrated that this strain was closely related to Pandoraea pnomenusa LMG 18087 T (96.7 % similarity), P. pulmonicola LMG 18016 T (96.5 %), P. apista LMG 16407 T (96.2 %), P. norimbergensis LMG 18379 T (96.1 %) and P. sputorum LMG 18819 T (96.0 %). Strain ATSB16 T shared 96.0-96.4 % sequence similarity with four unnamed genomospecies of Pandoraea. The major cellular fatty acids of the strain ATSB16 T were C 17 : 0 cyclo (33.0 %) and C 16 : 0 (30.6 %). Q-8 was the predominant respiratory quinone. The major polar lipids were phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylethanolamine and two unidentified aminophospholipids. Hydroxyputrescine and putrescine were the predominant polyamines. The genomic DNA G+C content of the strain was 64.0 mol%. On the basis of the results obtained from this study, strain ATSB16 T represents a novel species of the genus Pandoraea, for which the name Pandoraea thiooxydans sp. nov. is proposed. The type strain is ATSB16 T (5KACC 12757 T 5LMG 24779 T ).Currently, the genus Pandoraea consists of five species, namely Pandoraea pnomenusa, P. pulmonicola, P. apista, P. norimbergensis and P. sputorum, and four genomospecies (Coenye et al., 2000;Daneshvar et al., 2001). During a study of thiosulfate-oxidizing bacterial populations in sesame rhizosphere soils, one of the isolates, strain ATSB16 T , was subjected to phenotypic, genotypic and chemotaxonomic investigations in order to determine its taxonomic position.Rhizosphere soil samples were collected from sesame (Sesamum indicum L.) cultivated in fields in Jung ha-dong (35 u 10-179 N 128 u 49-569 E), Republic of Korea.Rhizosphere soil (soil still attached to the roots after gentle shaking) and fine roots (approximately 1 cm in length) from five plants were pooled into one sample, homogenized by thorough hand-mixing and used for enrichment isolation. Each rhizosphere soil sample (10 g) was added to 100 ml liquid mineral salts thiosulfate (MST) medium (Mukhopadhyaya et al., 2000) and incubated in a rotary shaker at 30 u C, in the dark to avoid any growth of phototrophic bacteria, until the colour of the indicator in the medium, bromcresol purple, changed to yellow. To isolate pure cultures, 10-fold dilutions (10 2 -10 6 ) were made with sterile deionized water and 0.2 ml aliquots were spread with sterile glass sticks on MST agar. Colonies that developed a yellow halo against a purple background, indicative of the production of sulfuric acid resulting fromThe GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain ATSB16 T is EF397578.

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Assignment of CDC Weak Oxidizer Group 2 (WO-2) to the Genus Pandoraea and Characterization of Three New Pandoraea Genomospecies

Cited by 74 publications

References 20 publications

Misidentification of Pandoraea sputorum Isolated from Sputum of a Patient with Cystic Fibrosis and Review of Pandoraea Species Infections in Transplant Patients

Misidentification of Pandoraea sputorum Isolated from Sputum of a Patient with Cystic Fibrosis and Review of Pandoraea Species Infections in Transplant Patients

Inhibition of co-colonizing cystic fibrosis-associated pathogens by Pseudomonas aeruginosa and Burkholderia multivorans

Pandoraea thiooxydans sp. nov., a facultatively chemolithotrophic, thiosulfate-oxidizing bacterium isolated from rhizosphere soils of sesame (Sesamum indicum L.)

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