Assessment of the Multi-mycotoxin-Binding Efficacy of a Carbon/Aluminosilicate-Based Product in an in Vitro Gastrointestinal Model

Abstract

A laboratory model, set to simulate the in vivo conditions of the porcine gastrointestinal tract, was used to study the small intestinal absorption of several mycotoxins and the effectiveness of Standard Q/FIS (a carbon/aluminosilicate-based product) in reducing mycotoxin absorption when added to multitoxin-contaminated diets. Mycotoxins were quickly absorbed in the proximal part of the small intestine at levels of 105 and 89% for fumonisins B1 and B2, respectively, 87% for ochratoxin A, 74% for deoxynivalenol, 44% for aflatoxin B1, and 25% for zearalenone. Addition of Standard Q/FIS to the diet (up to 2%, w/w) significantly reduced mycotoxin absorption, in a dose-dependent manner, up to 88% for aflatoxin B1, 44% for zearalenone, and 29% for the fumonisins and ochratoxin. Standard Q/FIS was ineffective in reducing deoxynivalenol uptake. These findings suggest that Standard Q/FIS can be used as a multitoxin adsorbent material to prevent the individual and combined adverse effects of mycotoxins in animals.

New mycotoxin adsorbents based on tri-octahedral bentonites for animal feed

“…Anyhow, this preliminary study revealed that more than half of the adsorbents showed high adsorbing capacity for AFB1 and lower for FB1, ZEN and OTA, while little DON adsorption was recorded. Our results are in agreement with other previous works in which several adsorbent materials, including smectites, activated carbons (AC), polymers such as cholestyramine and other commercial products were tested, and no adsorbent materials, with the exception of AC and cholestyramine, showed a relevant ability binding DON (Avantaggiato et al, 2003(Avantaggiato et al, , 2005(Avantaggiato et al, , 2007Döll et al, 2004;Ramos et al, 1996 a, b;Sabater-Vilar et al, 2007). Regarding AC, DON adsorption abilities vary widely depending on the type of carbonaceous substances and activation processes.…”

New mycotoxin adsorbents based on tri-octahedral bentonites for animal feed

“…Anyhow, this preliminary study revealed that more than half of the adsorbents showed high adsorbing capacity for AFB1 and lower for FB1, ZEN and OTA, while little DON adsorption was recorded. Our results are in agreement with other previous works in which several adsorbent materials, including smectites, activated carbons (AC), polymers such as cholestyramine and other commercial products were tested, and no adsorbent materials, with the exception of AC and cholestyramine, showed a relevant ability binding DON (Avantaggiato et al, 2003(Avantaggiato et al, , 2005(Avantaggiato et al, , 2007Döll et al, 2004;Ramos et al, 1996 a, b;Sabater-Vilar et al, 2007). Regarding AC, DON adsorption abilities vary widely depending on the type of carbonaceous substances and activation processes.…”

An In Vitro Study on the Efficacy of Mycotoxin Sequestering Agents for Aflatoxin B1, Deoxynivalenol, and Zearalenone

“…The great sequestering ability of two aluminosilicate products in the present work is likely due to the fact that aluminosilicates can form a stable complex with AFB1 at 37 • C [28]. In agreement with the present work, an aluminosilicate product has been shown to effectively bind to AFB1 [17,18]. Heulandite is categorized as a zeolite consisting of tectosilicates and has AFB1 sequestering ability [29], which supports the present results.…”

In Vitro Efficacy Assessment of Mycotoxin-Detoxifying Agents Against Emerging Mycotoxins

“…As expected, DON adsorption was not significant (it did not exceed 10%). The latter finding was also observed by several authors stating that except for AC and cholestyramine, no material had a relevant ability in binding DON [60,61,65,66,71,72].…”

New mycotoxin adsorbents based on tri-octahedral bentonites for animal feed

“…Anyhow, this preliminary study revealed that more than half of the adsorbents showed high adsorbing capacity for AFB1 and lower for FB1, ZEN and OTA, while little DON adsorption was recorded. Our results are in agreement with other previous works in which several adsorbent materials, including smectites, activated carbons (AC), polymers such as cholestyramine and other commercial products were tested, and no adsorbent materials, with the exception of AC and cholestyramine, showed a relevant ability binding DON (Avantaggiato et al, 2003(Avantaggiato et al, , 2005(Avantaggiato et al, , 2007Döll et al, 2004;Ramos et al, 1996 a, b;Sabater-Vilar et al, 2007). Regarding AC, DON adsorption abilities vary widely depending on the type of carbonaceous substances and activation processes.…”

An In Vitro Study on the Efficacy of Mycotoxin Sequestering Agents for Aflatoxin B1, Deoxynivalenol, and Zearalenone

“…The great sequestering ability of two aluminosilicate products in the present work is likely due to the fact that aluminosilicates can form a stable complex with AFB1 at 37 • C [28]. In agreement with the present work, an aluminosilicate product has been shown to effectively bind to AFB1 [17,18]. Heulandite is categorized as a zeolite consisting of tectosilicates and has AFB1 sequestering ability [29], which supports the present results.…”

In Vitro Efficacy Assessment of Mycotoxin-Detoxifying Agents Against Emerging Mycotoxins

“…As expected, DON adsorption was not significant (it did not exceed 10%). The latter finding was also observed by several authors stating that except for AC and cholestyramine, no material had a relevant ability in binding DON [60,61,65,66,71,72].…”

New mycotoxin adsorbents based on tri-octahedral bentonites for animal feed

“…Anyhow, this preliminary study revealed that more than half of the adsorbents showed high adsorbing capacity for AFB1 and lower for FB1, ZEN and OTA, while little DON adsorption was recorded. Our results are in agreement with other previous works in which several adsorbent materials, including smectites, activated carbons (AC), polymers such as cholestyramine and other commercial products were tested, and no adsorbent materials, with the exception of AC and cholestyramine, showed a relevant ability binding DON (Avantaggiato et al, 2003(Avantaggiato et al, , 2005(Avantaggiato et al, , 2007Döll et al, 2004;Ramos et al, 1996 a, b;Sabater-Vilar et al, 2007). Regarding AC, DON adsorption abilities vary widely depending on the type of carbonaceous substances and activation processes.…”

An In Vitro Study on the Efficacy of Mycotoxin Sequestering Agents for Aflatoxin B1, Deoxynivalenol, and Zearalenone

“…The great sequestering ability of two aluminosilicate products in the present work is likely due to the fact that aluminosilicates can form a stable complex with AFB1 at 37 • C [28]. In agreement with the present work, an aluminosilicate product has been shown to effectively bind to AFB1 [17,18]. Heulandite is categorized as a zeolite consisting of tectosilicates and has AFB1 sequestering ability [29], which supports the present results.…”

In Vitro Efficacy Assessment of Mycotoxin-Detoxifying Agents Against Emerging Mycotoxins

“…As expected, DON adsorption was not significant (it did not exceed 10%). The latter finding was also observed by several authors stating that except for AC and cholestyramine, no material had a relevant ability in binding DON [60,61,65,66,71,72].…”