Assessment of the influence of the composition of atmospheric microparticles on redox homeostasis of alveolar macrophages

Abstract

Introduction. The components and dimensions of suspended particulate matter (SPM) depend on territory, the sources of generation. The aim is to assess the relationship between the quality and dispersed composition of atmospheric SPMs with oxidative and antioxidant processes in alveolar macrophages. Materials and methods. The impact of actual multi component pollution of the surface layer of atmospheric air on alveolar macrophages (AMs) of Wistar rats was modelled. Correlations between the characteristics of AMs and pollution were assessed using the Spearman correlation coefficient. Results. The predominant contribution of the quality characteristics of SPM has been founded. The effect of the dispersed composition increases with an increase of the finely dispersed particles fraction. Metal particles have caused oxidative damage to lipids, with the formation of stable lipid products. The antioxidant system (AOS) has been characterized by the activation of compensatory response. Mineral components have caused irreversible oxidative damage to lipid and protein compounds, activating the thioredoxin formation. Soot particles have showed the most pathogenic effect, leading to oxidative damage to lipids, proteins, and nucleic acids. The AOS stress was accompanied by an increase in the formation of oxidized glutathione and thioredoxin, which performs restoration of proteins and DNA. Limitations. Our study characterizes the response of the AMs of a particular territory. Conclusion. The established tendencies characterize the impact of composition of SPMs of the urban atmospheric on AMs. The study made it possible to identify the most sensitive criteria for the response of AMs upon contact with atmospheric SPMs (thioredoxin, protein carbonyl, 8-hydroxy-2’-deoxyguanosine).

Effects Of Atmospheric Suspended Particulate Matter On The Immune System

“…The thioredoxin system ensures the restoration of disulfide bonds in proteins damaged by oxidation. Another example of redox regulation is the activation of the transcription factor NF-κB by cytosolic thioredoxin, which regulates the immune response, apoptosis and the cell cycle [53][54][55][56][57].…”

“…Production of ROS by SPM and formation of oxidative stress induce transmission of NF-κB and MAPK signals. Although both signaling pathways are activated upon exposure to SPM, the NF-κB pathway has been found to play a crucial role [57,[59][60][61][62]. Active oxygen species can directly affect cellular calcium channels, thereby disrupting the transmission of intracellular ionic signals.…”