Assessment of the genetic diversity of Two Czech autochthonous sheep breeds

Machová, Karolína; Milerski, M.; Rychtářová, Jana; Hofmanová, B.; Vostrá-Vydrová, Hana; Moravčíková, Nina; Kasarda, Radovan; Vostrý, L.

doi:10.1016/j.smallrumres.2020.106301

Cited by 4 publications

(3 citation statements)

References 37 publications

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“…It is worth noting that loss of genetic diversity within species and among populations is harmful not only in the context of conservation and current productivity but also for future utilization, as some genes lost at present might be vital in the future [ 18 ], under the dynamic global economic and social status and climate change as well. Perhaps it is also worth noting that species lose their genetic diversity through uncontrolled gene flow between and within populations leading to inbreeding, reduced effective population size, consequently causing inbreeding depression e.g., [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Meta-Analysis of Mitochondrial DNA Control Region Diversity to Shed Light on Phylogenetic Relationship and Demographic History of African Sheep (Ovis aries) Breeds

Wanjala

Bagi²,

Kusza³

2021

Biology

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To improve sheep breeding and conservation of genetic resources, the mitochondrial DNA control region (mtDNA CR) of 399 sequences of African indigenous sheep breeds from previously published research articles were meta-analyzed to elucidate their phylogenetic relationship, diversity, and demographic history. A total of 272 haplotypes were found, of which 207 were unique and a high level of mtDNA CR variability was observed. Generally, the number of polymorphic sites, nucleotide and haplotype diversity were high (284, 0.254 ± 0.012 and 0.993 ± 0.002, respectively). The median-joining (MJ) network of haplotypes produced three major haplogroups (A, B and C), with haplogroup B being dominant. A mixture of populations suggests a common matrilineal origin and lack of and/or a weak phylogeographic structure. Mismatch analysis showed recent expansion of North African breeds, whereas East African and continental populations exhibited selection pressures for adaptation. A slight historical genetic difference was also observed between the fat tail and thin tail sheep breeds. However, further investigations are required using more samples and long sequence segments to achieve deeper levels of conclusions on the African sheep phylogenetic relationship. The present meta-analysis results contribute to the general understanding of African native sheep populations for improved management of sheep genetic resources.

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Section: Introductionmentioning

confidence: 99%

Meta-Analysis of Mitochondrial DNA Control Region Diversity to Shed Light on Phylogenetic Relationship and Demographic History of African Sheep (Ovis aries) Breeds

Wanjala

Bagi²,

Kusza³

2021

Biology

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“…Wallachian sheep are classified among genetic resources and protected by the Czech national rescued program of threatened species for their historical and genetic uniqueness. Machova et al [ 1 ] estimated the average inbreeding coefficient at 5% and the average relatedness coefficient at 9% in a population of Wallachian sheep. The inbreeding coefficient has a cumulative effect and could reach as high an inbreeding coefficient as 7% over the next 30 years, as Machova et al [ 1 ] have added.…”

Section: Introductionmentioning

confidence: 99%

“…Machova et al [ 1 ] estimated the average inbreeding coefficient at 5% and the average relatedness coefficient at 9% in a population of Wallachian sheep. The inbreeding coefficient has a cumulative effect and could reach as high an inbreeding coefficient as 7% over the next 30 years, as Machova et al [ 1 ] have added. Sonesson et al [ 2 ] hypothesized that inbreeding can be reduced by a correctly designed strategy of frozen semen insemination when different generations are introduced in a closed small-scaled population.…”

Section: Introductionmentioning

confidence: 99%

Glycerol-Free Equilibration with the Addition of Glycerol Shortly before the Freezing Procedure: A Perspective Strategy for Cryopreservation of Wallachian Ram Sperm

Málková

Savvulidi

Ptáček

et al. 2023

Animals

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This study investigated the effect of glycerol added in different phases of sperm equilibration on CASA and flow cytometry parameters of thawed ram spermatozoa. Sperm was collected from adult Wallachian rams. The freezing extender was glycerol-free ANDROMED® (Minitub GmbH, Tiefenbach, Germany) supplied by 6% exogenous glycerol at different stages of the cryopreservation process. The purpose of this study was to compare two strategies of glycerol addition for sperm cryopreservation. The first strategy included the use of a glycerol-free extender for the procedure of glycerol-free equilibration and chilling, with the glycerolation of the extender by 6% glycerol shortly before sperm slow freezing (GFA). The second strategy included the use of a freezing extender already glycerolated by 6% glycerol before the equilibration and chilling of sperm and following slow freezing (GA). Sperm samples were analyzed after equilibration (but before freezing) and after thawing (at T0, T1 h, and T2 h time points). iSperm® mCASA (Aidmics Biotechnology Co., LTD., Taipei, Taiwan) was used for the evaluation of sperm kinematics. Flow cytometry was used to measure sperm viability (plasma membrane/acrosome intactness) and mitochondrial membrane potential. The obtained results significantly demonstrated that the glycerol-free equilibration with the addition of glycerol shortly before freezing is a perspective strategy for cryopreservation of Wallachian ram sperm.

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Genetic diversity of two native sheep breeds by genome-wide analysis of single nucleotide polymorphisms

Machová¹,

Marina²,

Arranz³

et al. 2023

animal

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Assessment of the genetic diversity of Two Czech autochthonous sheep breeds

Cited by 4 publications

References 37 publications

Meta-Analysis of Mitochondrial DNA Control Region Diversity to Shed Light on Phylogenetic Relationship and Demographic History of African Sheep (Ovis aries) Breeds

Meta-Analysis of Mitochondrial DNA Control Region Diversity to Shed Light on Phylogenetic Relationship and Demographic History of African Sheep (Ovis aries) Breeds

Glycerol-Free Equilibration with the Addition of Glycerol Shortly before the Freezing Procedure: A Perspective Strategy for Cryopreservation of Wallachian Ram Sperm

Genetic diversity of two native sheep breeds by genome-wide analysis of single nucleotide polymorphisms

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