Assessment of the Effect of Easily-metabolised Carbon Supplements on Xylanase Production by Newly Isolated Trichoderma asperellum USM SD4 Cultivated on Oil Palm Empty Fruit Bunches

Ajijolakewu, Kamoldeen Abiodun; Leh, Cheu Peng; Abdullah, Wan Nazwanie Wan; Lee, Chee Keong

doi:10.15376/biores.11.4.9611-9627

Cited by 13 publications

(14 citation statements)

References 27 publications

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“…Water and substrate powder will mix to form a tight bulk or pellet, which not only significantly reduces oxygen transfer, but also obstructs in-depth growth of fungal mycelium. Comparing to particle size, the important effect of initial moisture content on products production by SSF was widely demonstrated in many previous studies (Almeida et al 2019 ; Botella et al 2007 ; Yang et al 2006 ; Ajijolakewu et al 2016 ; Nutongkaew et al 2019 ; Ezeilo et al 2019 ). Our results show that 1:3 solid–liquid ratio (75% of moisture, Y = 11.75 mg/g, Fig.…”

Section: Resultsmentioning

confidence: 83%

“…RCK2010 cultivated on wheat bran at 30 °C. Trichoderma asperellum USM SD4 which grew on oil palm empty fruit bunch showed a lower optimum temperature of 27 °C for xylanase production (Ajijolakewu et al 2016 ). In fact, these basic findings revealed optimum growth temperatures between 25 and 30 °C for products production by SSF of filamentous fungi (Almeida et al 2019 ; Botella et al 2007 ; Yang et al 2006 ; Ajijolakewu et al 2016 ; Nutongkaew et al 2019 ; Ezeilo et al 2019 ).…”

Section: Resultsmentioning

confidence: 99%

“…Trichoderma asperellum USM SD4 which grew on oil palm empty fruit bunch showed a lower optimum temperature of 27 °C for xylanase production (Ajijolakewu et al 2016 ). In fact, these basic findings revealed optimum growth temperatures between 25 and 30 °C for products production by SSF of filamentous fungi (Almeida et al 2019 ; Botella et al 2007 ; Yang et al 2006 ; Ajijolakewu et al 2016 ; Nutongkaew et al 2019 ; Ezeilo et al 2019 ). Furthermore, from our work and previous studies, it must be pointed out that filamentous fungi are better than other microorganism, such as bacteria, for production of products by SSF due to their optimal incubation temperature closing to room temperature.…”

Section: Resultsmentioning

confidence: 99%

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Concurrent production of glycyrrhetic acid 3-O-mono-β-d-glucuronide and lignocellulolytic enzymes by solid-state fermentation of a plant endophytic Chaetomium globosum

Gao

Yang

Zhang

et al. 2021

Bioresour. Bioprocess.

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Glycyrrhetic acid 3-O-mono-β-d-glucuronide (GAMG) as an important derivative of glycyrrhizin (GL) shows stronger biological activities and higher sweetness than GL. The biotransformation process is considered as an efficient strategy for GAMG production, due to its mild reaction, high production efficiency and environmentally friendly status. In this study, licorice straw was used for the first time as a medium for GAMG and lignocellulosic enzyme production via solid-state fermentation (SSF) of endophytic fungus Chaetomium globosum DX-THS3. The fermentation conditions including particle size, temperature, seed age, inoculum size, and moisture of substrate were optimized. Furthermore, additional nitrogen sources and carbon sources were screened for GAMG production by C. globosum DX-THS3 of SSF. Under optimal fermentation conditions, the percent conversion of glycyrrhizin reached 90% in 15 days, whereas the control needed 35 days to achieve the same result. The productivity of optimization (P = 2.1 mg/g/day) was 2.33-fold that of non-optimization (P = 0.9 mg/g/day). Meanwhile, high activities of filter paper enzyme (FPase) (245.80 U/g), carboxymethyl cellulase (CMCase) (33.67 U/g), xylanase (83.44 U/g), and β-glucuronidase activity (271.42 U/g) were obtained faster than those in the control during SSF. Our study provides a novel and efficient strategy for GAMG production and indicates C. globosum DX-THS3 as a potential producer of lignocellulolytic enzymes.

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Section: Resultsmentioning

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Concurrent production of glycyrrhetic acid 3-O-mono-β-d-glucuronide and lignocellulolytic enzymes by solid-state fermentation of a plant endophytic Chaetomium globosum

Gao

Yang

Zhang

et al. 2021

Bioresour. Bioprocess.

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“…Xylose and xylan [6,40], as well as simple sugars derived from lignocellulosic substrates are effective xylanase inducers. While a mixture of these sugars was suggested to potentially improve production of xylanases [39], it remains unclear how a microorganism reacts to each individual inducer [24,39,47]. Here, we evaluated the effect of different xylooligosaccharides and cellooligosaccharides on induction of xylanolytic enzymes by F. oxysporum.…”

Section: Discussionmentioning

confidence: 99%

“…Such complexity can be attributed to the different physiological and metabolic properties of microorganisms, fermentation type (solid state or submerged fermentation), composition of culture medium, and carbon source (pure sugars or lignocellulosic substrates) [6,39]. In most instances where xylose and xylan induced xylanases, these substrates were added as pure carbon sources in submerged fermentation.…”

Section: Introductionmentioning

confidence: 99%

Effect of Oligosaccharide Degree of Polymerization on the Induction of Xylan-Degrading Enzymes by Fusarium oxysporum f. sp. Lycopersici

2020

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Xylan is one of the most abundant carbohydrates on Earth. Complete degradation of xylan is achieved by the collaborative action of endo-β-1,4-xylanases and β-d-xylosidases and a number of accessories enzymes. In filamentous fungi, the xylanolytic system is controlled through induction and repression. However, the exact mechanism remains unclear. Substrates containing xylan promote the induction of xylanases, which release xylooligosaccharides. These, in turn, induce expression of xylanase-encoding genes. Here, we aimed to determine which xylan degradation products acted as inducers, and whether the size of the released oligomer correlated with its induction strength. To this end, we compared xylanase production by different inducers, such as sophorose, lactose, cellooligosaccharides, and xylooligosaccharides in Fusarium oxysporum f. sp. lycopersici. Results indicate that xylooligosaccharides are more effective than other substrates at inducing endoxylanase and β-xylosidases. Moreover, we report a correlation between the degree of xylooligosaccharide polymerization and induction efficiency of each enzyme. Specifically, xylotetraose is the best inducer of endoxylanase, xylohexaose of extracellular β-xylosidase, and xylobiose of cell-bound β-xylosidase.

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Solid‐state valorization of raw oil palm leaves by novel fungi Trichoderma asperellumUC1 and Rhizopus oryzaeUC2 for sustainable production of cellulase and xylanase

Ezeilo

Wahab

Huyop

et al. 2021

J of Chemical Tech & Biotech

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BACKGROUND:The growing demand for cellulases and xylanases in lignocellulosic degradation and reutilization has spurred the need for their improved production at reduced cost. Abundantly available oil palm leaves (OPL) promise an alternative and sustainable substrate for producing such enzymes in the degradation and biotransformation of unwanted lignocellulosic biomass. Both enzymes are key players in the transformation of biomass into other value-added commodities.RESULTS: This study statistically optimized the solid-state fermentation (SSF) of raw OPL by novel fungal strains Trichoderma asperellum UC1 and Rhizopus oryzae UC2, to produce cellulase and xylanase, where raw OPL was pretreated using ultrasonication before the dual enzyme-assisted saccharification process. This experiment aimed to identify the best parameters for the highest activity of CMCase (EC 3.2.1.4), FPase (EC 3.2.1.91) and xylanase (EC 3.2.1.8) of T. asperellum UC1 and of ⊎-glucosidase (EC 3.2.1.21) of R. oryzae UC2 for improved saccharification. Activities of CMCase (126.87 U g −1 ), FPase (85.53 U g −1 ) and xylanase (215.42 U g −1 ) achieved the maximum under optimized SSF conditions (30 °C, 2.0 × 10 7 spores g −1 , 75% moisture content, pH 6). The best ⊎-glucosidase activity (131.76 U g −1 ) was obtained at 32 °C, 2.0 × 10 7 spores g −1 , 50% moisture content, pH 12. OPL saccharification yielded 1240 ± 32 mg g −1 total reducing sugar. Individual enzyme cocktails improved juice clarification (84-88%) and dough rising (1.7-to 2.0-fold).CONCLUSIONS: The optimized SSF of raw OPL successfully afforded the production of effective cellulases and xylanases for saccharification-related reactions.

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Assessment of the Effect of Easily-metabolised Carbon Supplements on Xylanase Production by Newly Isolated Trichoderma asperellum USM SD4 Cultivated on Oil Palm Empty Fruit Bunches

Cited by 13 publications

References 27 publications

Concurrent production of glycyrrhetic acid 3-O-mono-β-d-glucuronide and lignocellulolytic enzymes by solid-state fermentation of a plant endophytic Chaetomium globosum

Concurrent production of glycyrrhetic acid 3-O-mono-β-d-glucuronide and lignocellulolytic enzymes by solid-state fermentation of a plant endophytic Chaetomium globosum

Effect of Oligosaccharide Degree of Polymerization on the Induction of Xylan-Degrading Enzymes by Fusarium oxysporum f. sp. Lycopersici

Solid‐state valorization of raw oil palm leaves by novel fungi Trichoderma asperellumUC1 and Rhizopus oryzaeUC2 for sustainable production of cellulase and xylanase

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