Assessment of the Angiogenic Potential of 2-Deoxy-D-Ribose Using a Novel in vitro 3D Dynamic Model in Comparison With Established in vitro Assays

Dikici, Serkan; Dikici, Betül Aldemir; Bhaloo, Shirin Issa; Balcells, Mercedes; Edelman, Elazer R.; MacNeil, Sheila; Reilly, Gwendolen C.; Sherborne, Colin; Claeyssens, Frederik

doi:10.3389/fbioe.2019.00451

Cited by 34 publications

(57 citation statements)

References 93 publications

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“…Severn et al (2019) revealed that functionalised thiolene PolyHIPEs are promising platforms to mimic the bone marrow niche. Recently, Dikici et al developed a 3D dynamic in vitro model using tubular PCL PolyHIPEs combined with electrospun PCL tubes that can be used for the testing of angiogenic agents (Figure 12D; Dikici et al, 2020a). In the developed dynamic system, PCL PolyHIPE was found to be a suitable environment for comparison of the infiltration capacity of endothelial cells in response to different pro-angiogenic factors.…”

Section: In Vitro Modelsmentioning

confidence: 99%

“…This technique is the easiest way of manufacturing PolyHIPE scaffolds with almost no additional technical equipment requirement. Silicone ( Naranda et al, 2016 ; Aldemir Dikici et al, 2019a , b ; Dikici et al, 2020a ), polyvinyl chloride ( Busby et al, 2002 ), polytetrafluoroethylene ( Hayman et al, 2004 , 2005 ; Lee et al, 2017 ), glass ( Audouin et al, 2012 ), polypropylene ( Moglia et al, 2014b ), polycarbonate centrifuge tubes ( Carnachan et al, 2006 ; Hayward et al, 2013b ), and aluminium ( Moglia et al, 2011 ) are some of the materials reported to be used to create moulds for the fabrication of PolyHIPE scaffolds. Recently, we have shown that mould material has a significant impact on the morphology of the contact surface of PolyHIPEs which have fully or partially closed cellular morphology ( Aldemir Dikici et al, 2019a ).…”

Section: Development Of the Emulsion Templated Scaffoldsmentioning

confidence: 99%

“…(Original images were cropped, and scale bars were added to enhance the figures). Images (Ab,Bb,Cb,Fb) were adapted from Dikici et al (2020a) , Owen et al (2016) , Aldemir Dikici et al (2020) , and Paterson et al (2018) respectively, under The Creative Commons License. Image (Db) was adapted with permission from Moglia et al (2011) , Copyright 2011 American Chemical Society.…”

Section: Development Of the Emulsion Templated Scaffoldsmentioning

confidence: 99%

Section: Development Of the Emulsion Templated Scaffoldsmentioning

confidence: 99%

“…Casting enables the manufacturing of scaffolds in a wide range of shapes and sizes, depending on the mould design ( Paljevac et al, 2018 ; Diez-Ahedo et al, 2020 ; Dikici et al, 2020a ; Owen et al, 2020 ). Recently, Dikici et al (2020a) reported the fabrication of the PolyHIPEs in tubular form by designing a re-usable tubular silicone mould system that, HIPE can be injected into, polymerised, and recovered easily ( Figure 10Ab ). Also, sacrificial polymer beads made of PDMS ( Paljevac et al, 2018 ) or alginate ( Owen et al, 2020 ) have been incorporated into emulsion composition prior to polymerisation in a mould to simply introduce multiscale porosity to PolyHIPEs.…”

Section: Development Of the Emulsion Templated Scaffoldsmentioning

confidence: 99%

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Basic Principles of Emulsion Templating and Its Use as an Emerging Manufacturing Method of Tissue Engineering Scaffolds

Dikici

Claeyssens

2020

Front. Bioeng. Biotechnol.

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Tissue engineering (TE) aims to regenerate critical size defects, which cannot heal naturally, by using highly porous matrices called TE scaffolds made of biocompatible and biodegradable materials. There are various manufacturing techniques commonly used to fabricate TE scaffolds. However, in most cases, they do not provide materials with a highly interconnected pore design. Thus, emulsion templating is a promising and convenient route for the fabrication of matrices with up to 99% porosity and high interconnectivity. These matrices have been used for various application areas for decades. Although this polymer structuring technique is older than TE itself, the use of polymerised internal phase emulsions (PolyHIPEs) in TE is relatively new compared to other scaffold manufacturing techniques. It is likely because it requires a multidisciplinary background including materials science, chemistry and TE although producing emulsion templated scaffolds is practically simple. To date, a number of excellent reviews on emulsion templating have been published by the pioneers in this field in order to explain the chemistry behind this technique and potential areas of use of the emulsion templated structures. This particular review focusses on the key points of how emulsion templated scaffolds can be fabricated for different TE applications. Accordingly, we first explain the basics of emulsion templating and characteristics of PolyHIPE scaffolds. Then, we discuss the role of each ingredient in the emulsion and the impact of the compositional changes and process conditions on the characteristics of PolyHIPEs. Afterward, current fabrication methods of biocompatible PolyHIPE scaffolds and polymerisation routes are detailed, and the functionalisation strategies that can be used to improve the biological activity of PolyHIPE scaffolds are discussed. Finally, the applications of PolyHIPEs on soft and hard TE as well as in vitro models and drug delivery in the literature are summarised.

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Section: In Vitro Modelsmentioning

confidence: 99%

Section: Development Of the Emulsion Templated Scaffoldsmentioning

confidence: 99%

Section: Development Of the Emulsion Templated Scaffoldsmentioning

confidence: 99%

Section: Development Of the Emulsion Templated Scaffoldsmentioning

confidence: 99%

Section: Development Of the Emulsion Templated Scaffoldsmentioning

confidence: 99%

See 3 more Smart Citations

Basic Principles of Emulsion Templating and Its Use as an Emerging Manufacturing Method of Tissue Engineering Scaffolds

Dikici

Claeyssens

2020

Front. Bioeng. Biotechnol.

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Glass, Ceramic, Polymeric, and Composite Scaffolds with Multiscale Porosity for Bone Tissue Engineering

Jeyachandran

Cerruti

2023

Adv Eng Mater

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Porosity affects performance of scaffolds for bone tissue engineering both in vitro and in vivo. Macropores (i.e., pores with a diameter >100 μm) are essential for cellular infiltration; micropores (i.e., pores with a diameter of 1–10 μm) promote cell adhesion and facilitate nutrient absorption. Scaffolds containing both macropores and micropores exploit the advantages of both pore sizes and have excellent osteogenic properties. Nanopores (i.e., pores with a diameter of 1–50 nm) can be included as well, to improve cell–material interactions by further enhancing the surface area of the scaffold. This article reviews fabrication techniques and properties of scaffolds with multiscale porosity, focusing on glass, ceramic, polymeric, and composite scaffolds. After discussing the structure of bone and how it inspired scaffolds for bone tissue engineering, pore nomenclature is introduced. Then, the techniques used to induce multiscale porosity, the nature of the pores created, and the effects of scaffold porosity on mechanical properties and biological activity of the scaffolds are discussed. The review concludes by providing an outlook for this field, including advancements that are made possible by computational modeling and artificial intelligence.

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Microfluidic bubble‐generator enables digital light processing 3D printing of porous structures

Weber,

Cai,

Rojas

et al. 2023

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Three‐dimensional (3D) printing is an emerging technique that has shown promising success in engineering human tissues in recent years. Further development of vat‐photopolymerization printing modalities has significantly enhanced the complexity level for 3D printing of various functional structures and components. Similarly, the development of microfluidic chip systems is an emerging research sector with promising medical applications. This work demonstrates the coupling of a digital light processing (DLP) printing procedure with a microfluidic chip system to produce size‐tunable, 3D‐printable porosities with narrow pore size distributions within a gelatin methacryloyl (GelMA) hydrogel matrix. It is found that the generation of size‐tunable gas bubbles trapped within an aqueous GelMA hydrogel‐precursor can be controlled with high precision. Furthermore, the porosities are printed in two‐dimensional (2D) as well as in 3D using the DLP printer. In addition, the cytocompatibility of the printed porous scaffolds is investigated using fibroblasts, where high cell viabilities as well as cell proliferation, spreading, and migration are confirmed. It is anticipated that the strategy is widely applicable in a range of application areas such as tissue engineering and regenerative medicine, among others.

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Assessment of the Angiogenic Potential of 2-Deoxy-D-Ribose Using a Novel in vitro 3D Dynamic Model in Comparison With Established in vitro Assays

Cited by 34 publications

References 93 publications

Basic Principles of Emulsion Templating and Its Use as an Emerging Manufacturing Method of Tissue Engineering Scaffolds

Basic Principles of Emulsion Templating and Its Use as an Emerging Manufacturing Method of Tissue Engineering Scaffolds

Glass, Ceramic, Polymeric, and Composite Scaffolds with Multiscale Porosity for Bone Tissue Engineering

Microfluidic bubble‐generator enables digital light processing 3D printing of porous structures

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