Assessment of Salmonella spp. in feces, cloacal swabs, and eggs (eggshell and content separately) from a laying hen farm

García, Coralith; Castillo, José Miguel Soriano del; Benítez, V.; Catalá-Gregori, Pablo

doi:10.3382/ps.2010-01104

Cited by 46 publications

(38 citation statements)

References 21 publications

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“…This is relatively high compared to the results of other researchers [28,29,17] who demonstrated prevalence rates of 4% 19% and 26.3% respectively from poultry cloacal swabs. The relatively high prevalence rate of Salmonella from cloacal swabs as recorded by this study suggests that high number the poultry birds in Calabar are infected with the Salmonella species.…”

Section: Discussioncontrasting

confidence: 50%

Critical Segments in the Dissemination and Transmission of Salmonella Species from Poultry Production in Calabar, Nigeria

Yhiler¹,

Bassey²

2015

SJPH

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Salmonella species are ubiquitous enteric bacteria, its ability to survive at different stages of the poultry production and food processing chain has been frequently reported in recent years. The present study was undertaken to investigate the points in the segments involved in the production of poultry in Calabar as vital sources of dissemination and transmission of Salmonella species to humans. A total of 374 samples were collected from three study segments involved in the production of poultry in Calabar, within the period of August 2013 and May 2014. 170 samples were collected from different points in poultry environment which includes (feed from feeders, water from drinkers, litter/faeces from floor, abattoir reins and drag swab from wall); 136 samples were taken from the live birds (cloacal swab, gut, carcass and egg); and 68 samples were derived from poultry handlers (stool specimens and hand wash). The samples were collected aseptically and analysed for the presence of Salmonella species based on the ISO 6579:2002 involving standard bacteriological, biochemical and serologic techniques. The Chi-square, student t-test and simple descriptive statistics were used to analyse the data obtained in this study at 95% confidence level. Salmonella species were recovered from 221 (59.1%) of the examined samples involved in the production of poultry in Calabar. There was no significant statistical difference in the rate of recovery of Salmonella species from the poultry environmental segment (58.8%), bird segment (55.1%) and poultry handlers segment (67.6%) (P = 0.230), implying that these three segments are equally important in the maintenance, dissemination and transmission of Salmonella species. Salmonella species were recovered from all the study sample points implying that all the study poultry sample points are sources of Salmonella species. However, the highest recovery rate of Salmonella species was observed in the stool sample points of poultry handlers (91.2%) and poultry gut sample points (79.4%) which were significantly different from the rest of the poultry sample points (P = 0.021), implying that these two sample points are the major sources of Salmonella species and are critical in the dissemination and transmission of Salmonella species.

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Section: Discussioncontrasting

confidence: 50%

Critical Segments in the Dissemination and Transmission of Salmonella Species from Poultry Production in Calabar, Nigeria

Yhiler¹,

Bassey²

2015

SJPH

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“…detection by CB in cloacal swabs (1.6%) were lower than those reported by Singh et al (2013), who have found 4.4%, and by García et al (2011), which 4% of cloacal swabs were positive for this bacteria. Cloacal swabs used as samples to monitor the presence of Salmonella by conventional bacteriology present limitations.…”

Section: Resultscontrasting

confidence: 51%

Phenotypic and molecular detection of Salmonella sp. on growing, rearing and production phases in a commercial group of laying hens

et al. 2016

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This present study was developed with the objective of detect Salmonella sp. by conventional bacteriology and qPCR techniques in samples of flooring material from transport crates (meconium); raising environment (swab of cages and drinking fountains); cloacal swab; food and insects from growing, rearing and production phases in a commercial group of laying hens. A total of 864 samples were collected, among whom 248 originated from growing, 392 from rearing and 224 from production phase. Among the 864 samples, 2,8% where positives in bacteriologic technique and 15.3% in qPCR. Contamination was higher in growing and rearing phases and declined in production phase. Twenty four isolations of Salmonella where typified as Salmonella Agona (41.7%), Salmonella Livingstone (33.3%), Salmonella Cerro (16.7%), Salmonella Senftenberg (4.2%) and Salmonella Schwarzengrund (4.2%). During growing phase Salmonella Livingstone was identified. These findings suggest vertical contamination in the group. During rearing and production phases, isolated materials belong to serovars Agona, Cerro, Senftenberg and Schwarzengrund, pointing to horizontal contamination. It is possible to conclude that both vertical and horizontal contaminations are important during the cycle of commercial egg production and contamination in rearing phase is higher than in growing and production phases.

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“…from chicken faecal samples in Owerri metropolis Imo state, Nigeria. However, Garcia et al (2011) reported a lower isolation rate of Salmonella from laying hens in Aquidauana, Brazil. The results obtained in this study were consistent with the reports of other studies carried out in other parts of the world (Akond et al, 2012).…”

Section: Discussionmentioning

confidence: 97%

Multiple antimicrobial resistance of <i>Escherichia coli</i> and <i>Salmonella</i> species isolated from broilers and local chickens retailed along the roadside in Zaria, Nigeria

Ejeh¹,

Lawan²,

Abdulsalam³

et al. 2017

Sokoto J. Vet. Sc.

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have been shown to be resistant to multiple antibiotics. We carried out a bacteriological investigation on 105 cloacal swabs from local and broiler chickens retailed along the roadside in Hanwa, Zaria for the occurrence of E. coli and Salmonella spp. by isolation through culture, and identification using biochemical and serotyping techniques. Serotyping of E. coli isolates was carried out with polyvalent E. coli O157:H7 antisera while Salmonella spp. isolates were serotyped using polyvalent antisera specific for all groups and type-factor for Salmonella spp. Presumptive isolates were subjected to antimicrobial susceptibility testing using 13 panels of antibiotics for both E. coli and Salmonella spp. Results showed that the overall isolation rate of Salmonella spp. was 12 (11.4%), broiler chickens had higher isolation rate 9 (12.0%) of Salmonella than local chickens. However, the isolation rate of E. coli from local chickens 15 (50.0%) was higher compared to broilers 6 (8.0%). Also, non-E. coli O157:H7 and E. coli O157:H7 were isolated from both broilers and local chickens at a frequency of 15 (14.3%) and 6 (5.7%) respectively. The overall isolation rate of E. coli was 21 (20.0%). Multiple antibiotic resistance was observed among local and broiler chickens. However, E. coli and Salmonella species were 100% susceptible to Enrofloxacin. We therefore concluded that E. coli and Salmonella species are prevalent in the cloacae of both broiler and local chickens retailed along the roadside in Hanwa, Zaria, Nigeria and could pose serious veterinary and public health risks.

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Assessment of Salmonella spp. in feces, cloacal swabs, and eggs (eggshell and content separately) from a laying hen farm

Cited by 46 publications

References 21 publications

Critical Segments in the Dissemination and Transmission of Salmonella Species from Poultry Production in Calabar, Nigeria

Critical Segments in the Dissemination and Transmission of Salmonella Species from Poultry Production in Calabar, Nigeria

Phenotypic and molecular detection of Salmonella sp. on growing, rearing and production phases in a commercial group of laying hens

Multiple antimicrobial resistance of <i>Escherichia coli</i> and <i>Salmonella</i> species isolated from broilers and local chickens retailed along the roadside in Zaria, Nigeria

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