Assessment of polyglycolic acid mesh and bioactive glass for soft-tissue engineering scaffolds

Day, Richard M.; Boccaccını, Aldo R.; Shurey, Sandra; Roether, Judith A.; Forbes, Alastair; Hench, Larry L.; Gabe, Simon

doi:10.1016/j.biomaterials.2004.01.043

Cited by 300 publications

(212 citation statements)

References 26 publications

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“…6 The minimal medium containing different concentrations of crushed 45S5 Bioglass Ò -based scaffolds induced a significant proliferation of HUVECs. This observation is in line with previous studies, 6,10,11,14,16,42 which found a stimulating effect of 45S5 Bioglass Ò on endothelial cell proliferation. For example, Day 10 found that CM, produced by CCD-18Co fibroblasts grown on 45S5 Bioglass Ò , produced a significant increase in the number of endothelial cells after 24 h compared with endothelial cells grown in basal medium.…”

Section: Fig 2 Fibrous (B) and 45s5 Bioglasssupporting

confidence: 93%

“…[6][7][8][9] More recently, studies have shown that bioactive glasses may also be effective in promoting the vascularization of tissue constructs in different combinations, for example, as sintered scaffolds and composites. [10][11][12][13][14][15][16][17] The use of Bioglass Ò to fabricate scaffolds using a foam replica method was introduced in 2006 followed by studies on the possible angiogenic effect of such scaffolds. 18 Similar bioactive glass scaffolds were investigated in coculture studies using human umbilical vein endothelial cells (HUVEC) and human osteoblasts, 14 and it was shown that the scaffolds were able to support both endothelial and human osteoblast proliferation.…”

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confidence: 99%

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45S5-Bioglass^®-Based 3D-Scaffolds Seeded with Human Adipose Tissue-Derived Stem Cells Induce In Vivo Vascularization in the CAM Angiogenesis Assay

Handel

Hammer

Nooeaid

et al. 2013

Tissue Engineering Part A

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Poor vascularization is the key limitation for long-term acceptance of large three-dimensional (3D) tissue engineering constructs in regenerative medicine. 45S5 Bioglass Ò was investigated given its potential for applications in bone engineering. Since native Bioglass Ò shows insufficient angiogenic properties, we used a collagen coating, to seed human adipose tissue-derived stem cells (hASC) confluently onto 3D 45S5 Bioglass Ò -based scaffolds. To investigate vascularization by semiquantitative analyses, these biofunctionalized scaffolds were then subjected to in vitro human umbilical vein endothelial cells formation assays, and were also investigated in the chorioallantoic membrane (CAM) angiogenesis model, an in vivo angiogenesis assay, which uses the CAM of the hen's egg. In their native, nonbiofunctionalized state, neither Bioglass Ò -based nor biologically inert fibrous polypropylene control scaffolds showed angiogenic properties. However, significant vascularization was induced by hASC-seeded scaffolds (Bioglass Ò and polypropylene) in the CAM angiogenesis assay. Biofunctionalized scaffolds also showed enhanced tube lengths, compared to unmodified scaffolds or constructs seeded with fibroblasts. In case of biologically inert hernia meshes, the quantification of vascular endothelial growth factor secretion as the key angiogenic stimulus strongly correlated to the tube lengths and vessel numbers in all models. This correlation proved the CAM angiogenesis assay to be a suitable semiquantitative tool to characterize angiogenic effects of larger 3D implants. In addition, our results suggest that combinations of suitable scaffold materials, such as 45S5 Bioglass Ò , with hASC could be a promising approach for future tissue engineering applications.

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Section: Fig 2 Fibrous (B) and 45s5 Bioglasssupporting

confidence: 93%

mentioning

confidence: 99%

45S5-Bioglass^®-Based 3D-Scaffolds Seeded with Human Adipose Tissue-Derived Stem Cells Induce In Vivo Vascularization in the CAM Angiogenesis Assay

Handel

Hammer

Nooeaid

et al. 2013

Tissue Engineering Part A

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“…In vivo results have confirmed that BG is able to stimulate and promote neo-vascularization [52,55,[120][121][122]164,203,206,[208][209][210][211]. For example, Leu et al [211] filled calvarial defects in SpragueDawley rats with 45S5 Bioglass ® impregnated (1.2 mg) collagen sponges (volume = 0.05 cm 3 ) and unloaded, empty sponge as a control.…”

Section: Effect Of Bioactive Glass On Angiogenesismentioning

confidence: 92%

“…After two weeks of implantation, histological analyses of calvaria demonstrated significantly greater neo-vascularisation and vascular density within defects treated with 45S5 BG (35 ± 16 vessels/mm 2 ) than with collagen controls alone (12 ± 2 vessels/mm 2 ) ( Figure 17). The angiogenic effect of bioactive glass was, however, much more pronounced in bioactive glass-based scaffolds (i.e., loaded sponges [56], discs [208], meshes [203], tubes [209] and porous glass-ceramics scaffolds [52,121,122]) than in composite structures incorporating and fully embedding bioactive glass particles (e.g., microsphere composites [164], or foams [55,210]). However, in vivo results so far are inconsistent with in vitro results, and provide an incomplete picture concerning the suggested angiogenic potential.…”

Section: Effect Of Bioactive Glass On Angiogenesismentioning

confidence: 99%

Bioactive glass and glass-ceramic scaffolds for bone tissue engineering

Chatzistavrou

2011

Bioactive Glasses

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Traditionally, bioactive glasses have been used to fill and restore bone defects. More recently, this category of biomaterials has become an emerging research field for bone tissue engineering applications. Here, we review and discuss current knowledge on porous bone tissue engineering scaffolds on the basis of melt-derived bioactive silicate glass compositions and relevant composite structures. Starting with an excerpt on the history of bioactive glasses, as well as on fundamental requirements for bone tissue engineering scaffolds, a detailed overview on recent developments of bioactive glass and glass-ceramic scaffolds will be given, including a summary of common fabrication methods and a discussion on the microstructural-mechanical properties of scaffolds in relation to human bone (structure-property and structure-function relationship). In addition, ion release effects of bioactive glasses concerning osteogenic and angiogenic responses are addressed. Finally, areas of future research are highlighted in this review.

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“…15,17 The incorporation of 45S5 Bioglass Ò should lead to increased secretion of VEGF in vitro and increased neovascularization in vivo, as documented in the literature. 30 In a recent investigation, we were able to prove the possible combination of the intrinsic AVL model with an extrinsic vascular pathway using a newly developed porous titanium chamber to allow additional extrinsic vascularization of scaffolds via incorporated pores in the chamber design. 31 Further studies should combine 45S5 Bioglass Ò scaffolds with angiogenic growth factor and/or porous chambers to accelerate vascularization of matrices.…”

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Evaluation of Angiogenesis of Bioactive Glass in the Arteriovenous Loop Model

Arkudas¹,

Balzer²,

Buehrer³

et al. 2013

Tissue Engineering Part C: Methods

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In this study, the angiogenetic effect of sintered 45S5 Bioglass Ò was quantitatively assessed for the first time in the arteriovenous loop (AVL) model. An AVL was created by interposition of a venous graft from the contralateral side between the femoral artery and vein in the medial thigh of eight rats. The loop was placed in a Teflon isolation chamber and was embedded in a sintered 45S5 Bioglass Ò granula matrix filled with fibrin gel. Specimens were investigated 3 weeks postoperatively by means of microcomputed tomography, histological, and morphometrical techniques. All animals tolerated the operations well. At 3 weeks, both microcomputed tomography and histology demonstrated a dense network of newly formed vessels originating from the AVL. All constructs were filled with cell-rich, highly vascularized connective tissue around the vascular axis. Analysis of vessel diameter revealed constant small vessel diameters, indicating immature new vessel sprouts. This study shows for the first time axial vascularization of a sintered 45S5 Bioglass Ò granula matrix. After 3 weeks, the newly generated vascular network already interfused most parts of the scaffolds and showed signs of immaturity. The intrinsic type of vascularization allows transplantation of the entire construct using the AVL pedicle.

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Assessment of polyglycolic acid mesh and bioactive glass for soft-tissue engineering scaffolds

Cited by 300 publications

References 26 publications

45S5-Bioglass^®-Based 3D-Scaffolds Seeded with Human Adipose Tissue-Derived Stem Cells Induce In Vivo Vascularization in the CAM Angiogenesis Assay

45S5-Bioglass^®-Based 3D-Scaffolds Seeded with Human Adipose Tissue-Derived Stem Cells Induce In Vivo Vascularization in the CAM Angiogenesis Assay

Bioactive glass and glass-ceramic scaffolds for bone tissue engineering

Evaluation of Angiogenesis of Bioactive Glass in the Arteriovenous Loop Model

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Assessment of polyglycolic acid mesh and bioactive glass for soft-tissue engineering scaffolds

Cited by 300 publications

References 26 publications

45S5-Bioglass®-Based 3D-Scaffolds Seeded with Human Adipose Tissue-Derived Stem Cells Induce In Vivo Vascularization in the CAM Angiogenesis Assay

45S5-Bioglass®-Based 3D-Scaffolds Seeded with Human Adipose Tissue-Derived Stem Cells Induce In Vivo Vascularization in the CAM Angiogenesis Assay

Bioactive glass and glass-ceramic scaffolds for bone tissue engineering

Evaluation of Angiogenesis of Bioactive Glass in the Arteriovenous Loop Model

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Product

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45S5-Bioglass^®-Based 3D-Scaffolds Seeded with Human Adipose Tissue-Derived Stem Cells Induce In Vivo Vascularization in the CAM Angiogenesis Assay

45S5-Bioglass^®-Based 3D-Scaffolds Seeded with Human Adipose Tissue-Derived Stem Cells Induce In Vivo Vascularization in the CAM Angiogenesis Assay