Assessment of nanopolystyrene toxicity under fungal infection condition in Caenorhabditis elegans

Li, Dan; Deng, Yunjia; Wang, Zhen; Du, Huihui; Xiao, Guosheng; Wang, Dayong

doi:10.1016/j.ecoenv.2020.110625

Cited by 39 publications

(11 citation statements)

References 57 publications

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“…C. elegans lifespan was examined as described ( Li et al, 2020 ). After bacterial infection and Xuebijing treatment, the nematodes were transferred onto normal NGM plates.…”

Section: Methodsmentioning

confidence: 99%

Beneficial effect of Xuebijing against Pseudomonas aeruginosa infection in Caenorhabditis elegans

et al. 2022

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In the clinical intensive care units (ICU), the traditional Chinese medicine (TCM) formulation of Xuebijing has been frequently used for treating sepsis. Nevertheless, the underlying pharmacological mechanisms of Xuebijing remain largely unclear. Caenorhabditis elegans is an important experimental host for bacterial infections. Using C. elegans as an animal model, we here examined the potential of Xuebijing treatment against bacterial infection and the underlying mechanisms. Xuebijing treatment could inhibit the reduction tendency of lifespan caused by Pseudomonas aeruginosa infection. For the cellular mechanisms of this antibacterial infection property, we found that Xuebijing treatment rescued C. elegans lifespan to be against P. aeruginosa infection by inhibiting Pseudomonas colonization in the intestinal lumen. Meanwhile, the increase in the expression of antimicrobial genes induced by Pseudomonas infection was also suppressed by Xuebijing treatment. Moreover, the beneficial effect of Xuebijing against Pseudomonas infection depended on insulin, p38 MAPK, Wnt, DBL-1/TGF-β, ELT-2, and programmed cell death (PCD)-related signals. Although Xuebijing did not show obvious antibacterial activity, Xuebijing (100%) treatment could inhibit the Pseudomonas biofilm formation and decrease the expression of virulence genes (lasA, lasB, rhlA, rhlC, phzA, phzM, phzH, and phzS) and quorum sensing (QS)-related genes (lasI, lasR, rhlI, rhlR, pqsA, and pqsR). Our results support the potential role of Xuebijing treatment against bacterial infection in hosts.

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“…C. elegans lifespan was examined as described ( Li et al, 2020 ). After bacterial infection and Xuebijing treatment, the nematodes were transferred onto normal NGM plates.…”

Section: Methodsmentioning

confidence: 99%

Beneficial effect of Xuebijing against Pseudomonas aeruginosa infection in Caenorhabditis elegans

et al. 2022

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“…Mitrano et al (2014) also used DLS to confirm the nominal particle size of Ag-NPs. At present, SEM or TEM and DLS have been used to separate and characterize the NPs in zebrafish, Caenorhabditis elegans and Daphnia in freshwater, and then to study the toxicology of NPs (Li et al, 2020c;Zhang et al, 2020a;Qu et al, 2019a;Qiu et al, 2020a;Qiu et al, 2020b;Li et al, 2020d;Qu et al, 2020). Li et al (2016) used UV-vis spectrophotometry to determine the absorbance of TiO 2 -NPs, and then studied the deposition behavior of TiO 2 -NPs.…”

Section: Spectroscopy and Mass Spectrometrymentioning

confidence: 99%

“…In most of the studies on the biological toxicity of NPs, we found that they used PS-NPs as the research object (Qiu et al, 2020a;Liu et al, 2020;Li et al, 2020c;Zhang et al, 2020a;Qu et al, 2019a). It may be because the production of PS-NPs is relatively large, easy to obtain, and can also be traced by fluorescent labeling.…”

Section: Toxicity Of Nps In Freshwater Ecosystemmentioning

confidence: 99%

“…When the nanoplastics enter the organism, the pollutants attached to the surface and the nanoplastics will jointly induce toxicity accumulation (Koelmans et al, 2015). Li et al (2020c) studied the joint toxicity of fungal infected nematodes exposed to 30 nm PS-NPs (10 μ g/L and 100 μ g/L) for 24 h. The results showed that the life span, mating behavior, production of reactive oxygen species, the expression of sod-3: GFP and hsp-6: GFP, and the formation of fungal colonies in the intestinal tract of the nematodes exposed to C. elegans were significantly higher than those of the nematodes exposed to PS-NPs alone or exposed to PS-NPs. Ma et al (2016) used radiotracer to study the joint toxicity of NPs and phenanthrene.…”

Section: Joint Toxicity Of Nps and Other Pollutants To Freshwater Organismsmentioning

confidence: 99%

“…However, some studies defined NPs as plastics with particle size between 1 and 1000 nm (Sobhani et al, 2020;Gigault et al, 2018). Although there are some controversies about the definition of NPs, the definition of NPs with a particle size of 1-100 nm is generally accepted by researchers (Gigault et al, 2018;Li et al, 2020c;Zhang et al, 2020a;Qu et al, 2019a). NPs have higher cell affinity, smaller size and surface curvature, and higher surface area and volume, making it easier to penetrate freshwater biological barriers and accumulate in organs (Au et al, 2017;Kolandhasamy et al, 2018;Li et al, 2020a).…”

Section: Introductionmentioning

confidence: 99%

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