Assessment of in vivo attachment/phagocytosis by alveolar macrophages

Weaver, Todd; Hall, Christopher L.; Kachel, Diane L.; Ward, Robert P.; Williams, Mark D.; Perry, Douglas G.; Wisniowski, Paul; Martin, William J.

doi:10.1016/0022-1759(96)00031-2

Cited by 11 publications

(12 citation statements)

References 31 publications

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“…The results indicate that a greater proportion of reconstituted AMs were located in the caudal portion of the lung, similar to previous studies indicating the location of i.t.-delivered fluorescent beads (19). The right lower lobe had an evenness index score of 158.6 Ϯ 11.6; whereas, the middle and upper lobes had scores of 66.1 Ϯ 12.3 and 51.2 Ϯ 14.1, respectively ( p Ͻ 0.05 either comparison to caudal lobe) (Fig.…”

Section: Resultssupporting

confidence: 90%

“…administered into SCID mice (19). Recipient BALB/c SCID mice (Harlan Sprague Dawley) were anesthetized with 0.15 ml of an anesthetic mixture consisting of 10 mg/ml ketamine (Bedford Laboratories, Bedford, OH), 0.11 mg/ml atropine (Butler, Columbus, OH), and 2 mg/ml acepromazine (Butler).…”

Section: Reconstitution Of Amsmentioning

confidence: 99%

“…The cell suspension was followed by 200 l of air to clear the angiocath and upper respiratory tract. To ensure even distribution of the AMs, the mice were mechanically ventilated as previously described from our laboratory (19). The mouse was maintained on the ventilator (Analytic Specialties, St. Louis, MO) that was directly connected to the airway catheter.…”

Section: Reconstitution Of Amsmentioning

confidence: 99%

“…Lung tissue of similar mass without any reconstituted cells was used as a background. Data are presented as an evenness index as previously described (19). The evenness index is the ratio of ((fluorescence/ gram wet mass piece )/(fluorescence/gram wet mass whole )) ϫ 100.…”

Section: Distribution Of Ams In the Lungsmentioning

confidence: 99%

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Morphologic Detection and Functional Assessment of Reconstituted Normal Alveolar Macrophages in the Lungs of SCID Mice

Pasula

Weaver

Martínez

et al. 2002

The Journal of Immunology

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Alveolar macrophages (AMs) from immunocompetent animals were isolated from bronchoalveolar lavage and labeled with the fluorescent marker 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI). These AMs were administered intratracheally into mechanically ventilated SCID mice. From 1 to 28 days later, the recipient mice underwent bronchoalveolar lavage to isolate their AMs. To determine whether reconstituted AMs were still immunocompetent, the recovered AMs were assayed for their ability to phagocytose fluorescein-labeled zymosan-coated beads. After incubation with the beads, samples were assayed using a fluorescent-activated cell sorter to identify DiI-labeled reconstituted AMs, unlabeled resident AMs, and the proportion of these two groups undergoing phagocytosis. DiI-labeled AMs accounted for ∼50% of all returned AMs. Additionally, the reconstituted AMs from normal BALB/c mice retained phagocytic activity compared with AMs from immunodeficient SCID mice. Reconstituted AMs demonstrated enhanced phagocytic activity compared with resident SCID AMs for up to 28 days following reconstitution. These results indicate that immunocompetent AMs can be successfully reconstituted into an immunodeficient host to partially restore alveolar host defense.

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Section: Resultssupporting

confidence: 90%

Section: Reconstitution Of Amsmentioning

confidence: 99%

Section: Reconstitution Of Amsmentioning

confidence: 99%

Section: Distribution Of Ams In the Lungsmentioning

confidence: 99%

See 2 more Smart Citations

Morphologic Detection and Functional Assessment of Reconstituted Normal Alveolar Macrophages in the Lungs of SCID Mice

Pasula

Weaver

Martínez

et al. 2002

The Journal of Immunology

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“…IFN-␥ knockout mice were obtained from The Jackson Laboratory. Mice were anesthetized with 0.15 mg ketamine i.m., tracheotomized by using a 20-gauge angiocath, and ventilated by using a small animal ventilator for 10 min (Analytical Specialties), as previously described (31 Lung Harvesting for Histological Examination. At designated time points, mice were killed by i.p.…”

Section: Instillation Of Ifn-␥-expressing Macrophages Into Mouse Lungsmentioning

confidence: 99%

Genetically engineered macrophages expressing IFN-γ restore alveolar immune function inscidmice

Hussain

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

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Reversal of immunodeficiency in the lung by gene therapy is limited in part by the difficulty of transfecting lung cells in vivo.Many options exist for successfully transfecting cells in vitro, but they are not easily adapted to the in vivo condition. To overcome this limitation, we transduced macrophages in vitro with the murine IFN-␥ (mIFN-␥) gene and intratracheally delivered the macrophages to express mIFN-␥ in vivo. A recombinant retroviral vector pSF91 system was modified to encode mIFN-␥ and enhanced green fluorescent protein (EGFP). A murine macrophage cell line J774A.1 transduced with the retroviral supernatant increased secretion from undetectable levels to 131.6 ؎ 4.2 g/ml mIFN-␥ at 24 h in vitro. The mIFN-␥-producing macrophages were intratracheally instilled into mechanically ventilated scid mice. mIFN-␥ levels in the bronchoalveolar lavage increased from undetectable levels at baseline to 158.8 ؎ 5.1 pg/ml at 48 h (P < 0.001). Analysis of the lavaged cells for EGFP expression revealed that EGFP expression was directly proportional to the number of transduced macrophages instilled into the lung. Immune function was partially restored in the alveolar spaces of scid mice with evidence of enhanced MHC class II antigen expression and increased phagocytosis (P < 0.05). Tumor necrosis factor ␣ was increased from undetectable at baseline to 103.5 ؎ 11.4 pg/ml. In contrast, i.p. administration of the engineered macrophages did not enhance IFN-␥ levels in the lung. Our study suggests airway delivery of genetically engineered macrophages expressing mIFN-␥ gene can partially restore significant immune activity in the lungs of immunodeficient mice.

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Optimum conditions for efficient phagocytosis of rifampicin-loaded PLGA microspheres by alveolar macrophages

Hirota

Hasegawa

Hinata

et al. 2007

Journal of Controlled Release

154

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Assessment of in vivo attachment/phagocytosis by alveolar macrophages

Cited by 11 publications

References 31 publications

Morphologic Detection and Functional Assessment of Reconstituted Normal Alveolar Macrophages in the Lungs of SCID Mice

Morphologic Detection and Functional Assessment of Reconstituted Normal Alveolar Macrophages in the Lungs of SCID Mice

Genetically engineered macrophages expressing IFN-γ restore alveolar immune function inscidmice

Optimum conditions for efficient phagocytosis of rifampicin-loaded PLGA microspheres by alveolar macrophages

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