Assessment of in vitro sperm characteristics and their importance in the prediction of conception rate in a bovine timed-AI program

Oliveira, Letícia Zoccolaro; Arruda, Rubens Paes de; Andrade, André Furugen Cesar de; Celeghini, Eneiva Carla Carvalho; Reeb, Pablo; Martins, J.P.N.; Santos, Ricarda Maria dos; Beletti, Marcelo Emı́lio; Peres, R. F. G.; Monteiro, Fábio Morato; Lima, Vera Fernanda Martins Hossepian de

doi:10.1016/j.anireprosci.2013.01.010

Cited by 62 publications

(61 citation statements)

References 32 publications

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“…Plasma membrane functionality measured by the hypo-osmotic swelling test (HOST), sperm plasma membrane integrity and acrosomal integrity were considered good predictors of conception rate, also confirmed that the combination of Eosin/Negrosin staining test and HOST was highly correlated with in vitro fertility; and showed that when sperm plasma and acrosomal integrity (assessed by Trypan/Blue Giemsa staining) a higher correlation coefficient was obtained, also the morphological variables identified as important predictors of fertility [26].…”

Section: Discussionmentioning

confidence: 53%

Pure Egyptian Cattle Bulls Show both Individual Variation and Different Interaction with Extender in the Post-Thawing Sperm Parameters

Mohammed¹,

Ahmed²

2017

Andrology

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Objectives: As very few studies were done on the freezability of pure Egyptian cattle bull sperm. So, we designed this study to evaluate the individual variations in freezability of native bull semen extended in Tris based diluent and sodium citrate-based diluent. Methods:Semen was collected by artificial vagina, examined at once in farm laboratory. Only semen samples fit the minimum parameters are extended in two extenders first the universal one (TRIS) and second modified Sodium citrate extender by adding glycerol (CU-16) which created at Cornell University to evaluate the individual bull variation and interaction between extender and bull.Results: Post-Thawing individual sperm motility, live, abnormal, acrosome integrity percentage was evaluated in addition to Hypo-Osmotic Swelling test (HOS). The highest value for motility, live abnormal and acrosome percentage were 44.00 ± 1.12, 52.25 ± 1.60, 21.25 ± 0.81 and 62.80 ± 2.58 from bull 2, 1, 3 and 2, respectively for semen extended in TRIS, and 42.25 ± 1.61, 52.00 ± 1.76, 22.15 ± 0.85 and 57.40 ± 3.07, from bull 1, 1, 4, 3, respectively for semen extended in CU-16. The results of HOS were 59.25 ± 1.76 and 55.95 ± 2.13 from bull 1 extended in TRIS and CU-16, respectively. Conclusion:A significant variation (p<0.05) in tested parameter was clear when semen extended in TRIS extender with the absence of such variation when semen extended in CU-16 except in live sperm percentage. With ignoring the extender effect a clear significant variations were detected between bulls in all tested parameters.

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Section: Discussionmentioning

confidence: 53%

Pure Egyptian Cattle Bulls Show both Individual Variation and Different Interaction with Extender in the Post-Thawing Sperm Parameters

Mohammed¹,

Ahmed²

2017

Andrology

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“…Therefore, similar to the cervix and oviduct, Percoll may act as a challenging barrier for spermatozoa, operating as sperm selection technique for excluding sperm cells less capable in performing successful fertilization. There is good evidence that morphologically normal spermatozoa offer the best fertilizing potential (POUSETTE et al, 1986; VAN DER ZWALMEN et al, 1991;ARRUDA et al, 2011;OLIVEIRA et al, 2013). Van Der Zwalmen et al (1991) demonstrated that Percoll technique selects sperm samples with better morphological quality from normal as well as from poor semen samples.…”

Section: Resultsmentioning

confidence: 99%

“…Similarly, several studies already demonstrated varied conception rates depending on the semen batch and/ or sire utilized (CORREA et al, 1997;ZHANG et al, 1999;ANDERSSON et al 2004;SÁ FILHO et al, 2009;OLIVEIRA et al, 2013;SANTOS, 2016). According to Sellem et al (2015), predicting in vivo fertility of bull ejaculates based on in vitro semen characteristics remains challenging for the breeding industry.…”

Section: Introductionmentioning

confidence: 92%

“…However, classic and modern laboratory tests of semen quality are still unable to accurately estimate real sperm-fertilizing capacity. Though clear that identifying a practical in vitro semen analysis for predicting field fertility would be of great benefit to the semen industry, it is unlikely that the assessment of a single sperm characteristic may reflect the real fertilizing capacity of a semen sample (ARRUDA et al, 2011;OLIVEIRA et al, 2013), especially All cows received the same timed-AI protocol for first service. This protocol started (Day 0) with insertion of a new intravaginal progesterone-releasing device (Sincrogest ® ; Ouro Fino Saúde Animal, Cravinhos, SP, Brazil) and 2.0 mg of estradiol benzoate im (Sincrodiol ® ; Ouro Fino Saúde Animal).…”

Section: Introductionmentioning

confidence: 99%

“…Hence, multiple independent variables are often necessary in order to make a reasonably accurate prediction (UTT, 2016). For this reason, it is necessary to associate different semen quality tests so to improve the accuracy of a bull's reproductive potential (JANUSKAUSKAS et al, 2003;TARTAGLIONE;RITTA, 2004;THUNDATHIL, 2008;ARRUDA et al, 2011;OLIVEIRA et al, 2013;SELLEM et al, 2015;CELEGHINI et al, 2017).…”

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confidence: 99%

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Assessment of different in vitro sperm challenges and in vivo fertility of bovine semen batches

Oliveira

Ribeiro

Silva

et al. 2017

Braz. J. Vet. Res. Anim. Sci.

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The aim of this work was to submit sperm cells to different laboratory challenges and to compare in vitro results with in vivo semen fertility. Four different batches from the same Brangus bull were used in a timed-AI program of 332 Brangus cows. Each batch (B) was submitted to the following procedure: semen sample was thawed at 36°C for 30 seconds (control). Sperm motility parameters, plasma membrane integrity, sperm morphology, and concentration were assessed. Then, an aliquot of thawed sample was incubated in a water bath at 45°C for 40 min (thermal challenge group; TCG) and another aliquot was centrifuged at 500 xg (Percoll gradient 45%/90%) for 15 min (centrifugation challenge group; CCG). Centrifuged semen was also submitted to another thermal challenge, being incubated (water bath) at 45°C for 40 min (centrifugation + thermal challenge group; CTCG). At the end of each challenge (CCG, TCG, and CTCG), the same laboratory tests used for control group were repeated. The following conception rates (CR) were observed for each batch: B1 = 48.9% (44/90); B2 = 44.2% (23/52); B3 = 55.5% (40/72); B4 = 43.2% (51/118); (p < 0.10). In the lab, B3 presented higher (p ≤ 0.05) progressive motility (PM) than B4 after thawing (control group) and after all sperm challenges (TCG, CCG, and CTCG). However, despite B3 and B4 having demonstrated a similar percentage of plasma membrane integrity (PMI) to the control group (B3 = 66.7 ± 1.3 and B4 = 65.2 ± 3.3), B3 demonstrated higher (P ≤ 0.05) percentage of PMI (37.2 ± 2.5) than B4 (26.7 ± 3.3) after passing through the most stressing in vitro challenge (CTCG). The semen batch presenting the highest resistance to in vitro challenges was the one that presented a trend for higher in vivo fertility, suggesting that submitting semen samples to laboratory challenges may be an interesting alternative for selecting batches with greater field fertility. Keywords: Conception rate. In vitro sperm resistance. Timed-AI. ResumoO objetivo deste estudo foi estressar células espermáticas em diferentes desafios laboratoriais e comparar os resultados in vitro com a fertilidade in vivo do sêmen. Quatro partidas de um mesmo touro Brangus foram utilizadas em um programa de IATF de 332 vacas Brangus. Cada partida foi submetida ao seguinte procedimento: a amostra de sêmen foi descongelada a 36°C por 30 segundos (grupo controle). Foram avaliados parâmetros de motilidade espermática (CASA), integridade da membrana plasmática (PMI), morfologia e concentração espermática. Em seguida, uma alíquota da amostra descongelada foi incubada em banho-maria a 45°C durante 40 minutos (grupo de desafio térmico, TCG) e outra alíquota foi centrifugada a 500 xg (gradiente de Percoll 45%/90%) durante 15 min (grupo desafio de centrifugação, CCG). Uma aliquota do sêmen centrifugado foi ainda submetida ao desafio térmico, sendo incubado a 45°C durante 40 min (grupo de desafio térmico + centrifugação, CTCG). No final de cada desafio (CCG, TCG e CTCG), os mesmos testes laboratoriais utilizados para o grupo de controle f...

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Characterization of seminal parameters, sperm morphometry, micromorphology, and ultrastructure in gray brocket deer (Mazama gouazoubira, Fischer, 1814)

Cunha

Souza

Brito

et al. 2020

Microscopy Res & Technique

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Populations of gray brocket deer (Mazama gouazoubira) are declining; yet, knowledge on the reproductive biology of this species remains limited. Therefore, this study aimed to describe morphology, viability, membrane integrity, mitochondrial activity, morphometry, micromorphology, and ultrastructure of the gray brocket deer sperm. Three adult male gray brocket deer were used in the study. Semen collection was performed using electroejaculation. Semen were analyzed by evaluating pH, motilities, vigor, mass movement, volume, concentration, viability, membrane integrity, mitochondrial activity, morphology, and morphometry. Micromorphology and ultrastructure of sperm were analyzed using scanning and transmission electron microscopy (SEM and TEM), respectively. There was no significant difference among males regarding on pH, motilities, vigor, mass movement, volume, concentration, viability. High values for membrane integrity, mitochondrial activity, and normal sperm were observed. The most frequent defects were simple bent tail and bowed midpiece. The head length, and width, midpiece, and tail length were 8.5, 4.4, 11.5, and 41.3 μm, respectively. SEM sperm showed paddle‐shaped heads, with apical ridge and serrated band on the equatorial segment. TEM revealed the nucleus, acrosome, plasma membrane, mitochondria sheath, proximal centrioles, segmented columns, axoneme, outer dense fibers, and fibrous sheath. SEM and TEM showed the presence of some abnormalities. These results are expected to provide baseline values of diverse semen parameters, contributing toward the development of reproductive biotechnologies for gray brocket deer and, other deer species at risk of extinction.

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Assessment of in vitro sperm characteristics and their importance in the prediction of conception rate in a bovine timed-AI program

Cited by 62 publications

References 32 publications

Pure Egyptian Cattle Bulls Show both Individual Variation and Different Interaction with Extender in the Post-Thawing Sperm Parameters

Pure Egyptian Cattle Bulls Show both Individual Variation and Different Interaction with Extender in the Post-Thawing Sperm Parameters

Assessment of different in vitro sperm challenges and in vivo fertility of bovine semen batches

Characterization of seminal parameters, sperm morphometry, micromorphology, and ultrastructure in gray brocket deer (Mazama gouazoubira, Fischer, 1814)

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