Assessment of Genetic Variability of 142 Sweet Sorghum Germplasm of Diverse Origin with Molecular and Morphological Markers

Abstract

Sorghum bicolor (L.) Moech is the fifth most important crop in the world. Recently, its agronomics and genetics have drawn interest among scientists. Sweet sorghum, a variety of sorghum, may potentially become a bioenergy source because of the high sugar content in its juicy stems. Exploring the diversity of sweet sorghum around the world is important to the development and improvement of the crop as an energy source. In exploring the diversity of sweet sorghum, three types of markers (simple sequence repeats [SSR], sequence-related amplified polymorphisms [SRAP], and morphological markers) are used on 142 sweet sorghum accessions from around the world. The accessions show a high significance (P < 0.05) for all the morphological traits measured. The morphological markers cluster the accessions into five groups based primarily on plant height (PH), anthesis data (AD), and moisture content (ML), with the principal component analysis (PCA) showing these traits to explain 92.5% of the total variation. The furthest accessions were PI571103 from Sudan, and N99 from the United States. The Nei's genetic standard distances ranged from 0.024 to 1.135 and 0.078 to 0.866 for SSR and SRAP, respectively. As expected, accessions of the same origin or breeding history had the lowest genetic distance (e.g. Mokula and Marupantse, both from Botswana; NSL83777 and NSL83779 from Cameroon). Neighbor joining clusters the sweet sorghum accessions into five major groups using SSR and four major groups using SRAP, based on their origin, or breeding history. The three marker types complement each other, and the presence of accessions of different origins across clusters indicate similar genetics, and evidence of germplasm movement between countries.

Genetic Variability of 15 Robusta Coffee Genotypes Selected by Farmer Based on SSRs Markers

“…The polymorphic level obtained in this research ranged from 0.06 to 0.87, with an average of 0.57 (Table 2), higher than those obtained by Missio et al (2009) which is 0.46 but slightly less than those of the previous study on Robusta coffee germplasms in which 0.60 (Hendre & Aggrawal, 2014). Of which, the polymorphic level value higher than 0.5 considered as highly informative (Prabakaran, Paramasivam, Rajesh, & Rajarajan, 2010;Lekgari & Dweikat, 2014). Twenty three (67.65%) out of 34 SSR markers used in present study that showed high degree of polymorphism were potentially selected for future assessment of Robusta coffee germplasms.…”

Development of genomewide simple sequence repeat fingerprints and highly polymorphic markers in cucumbers based on next‐generation sequence data

“…DNA polymorphisms are the basis of the development of molecular markers such as SNPs and SSRs, and PIC is a key parameter in the evaluation of the performance of these molecular markers. In terms of genetic diversity, a marker with a PIC >0.5 is considered to be highly informative (Lekgari and Dweikat ). The most outstanding advantage of the SSR markers developed in this study is the high levels of polymorphism (PIC ≥0.5).…”

Assessment of genetic diversity in sorghum bicolor using RAPD markers

“…Due to the impact of sorghum as one of the most important cereal crops around the world, it is possible to find a wide number of studies related to determination of genetic diversity of this species (AKRAM et al, 2011;BAHAA et al, 2013;DAHLBERG, 2011;LEKGARI and DWEIKAT, 2014;MADHUSUDHANA et al, 2012;MBEYAGALA et al, 2012;MOFOKENG et al, 2012;RITTER et al, 2007). In this sense, the present study is aimed to assess the genetic diversity of sorghum genotypes from the Czech Plant Gene Bank, Crop Research Institute in Prague, through Random Amplified Polymorphic DNA molecular markers (RAPD).…”