Assessment of Genetic Variability in Two North Indian Buffalo Breeds Using Random Amplified Polymorphic DNA (RAPD) Markers

Sodhi, Monika; Mukesh, Manishi; Anand, A.; Bhatia, S. K.; Mishra, Bina

doi:10.5713/ajas.2006.1234

Cited by 5 publications

(3 citation statements)

References 18 publications

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“…The percentage of the primers screened that were amplification in the present study was much higher than that reported by Sodhi et al (2006). Of the 50 primers they used to screen two buffalo breeds, only 26 generated reproducible banding patterns.…”

Section: Discussioncontrasting

confidence: 57%

“…It is difficult to find monomorphic, unique RAPD markers for species, breed or population distinction and thus identification due to its highly polymorphic nature. Sodhi et al (2006) found two RAPD markers that were present in the NiliRavi buffalo breed but absent in the Murrah buffalo, however, these were polymorphic. Similarly, in the study comparing catla (Catla catla) from three major river systems, Islam et al (2005) found two RAPD markers unique to the Halda river population and one RAPD marker unique to the Padma river.…”

Section: Discussionmentioning

confidence: 90%

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RAPD Analysis of Three Deer Species in Malaysia

El-Jaafari¹,

Panandam²,

Idris³

et al. 2008

Asian Australas. J. Anim. Sci

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The genetic variability within and among three deer species in Malaysia, namely Cervus nippon (sika), Cervus timorensis (rusa) and Cervus unicolor (sambar), were evaluated using the RAPD technique. The DNA extracted from the buffy coat of 34 sika, 38 rusa and 9 sambar were analysed using ten primers that gave bands which showed good resolution. The primers generated 164 RAPD markers in total, and these ranged in size from 150 to 900 bp. The percent of polymorphism of the bands generated per primer ranged from 66.66-93.33% for rusa, 36.84-61.14% for sambar and 52.38-100% for sika. The overall percent polymorphism observed for the 164 RAPD markers was 99.39%. The results revealed five exclusive, monomorphic markers for sambar and one exclusive, monomorphic marker for sika; none was observed for rusa. However, these cannot be declared as markers for the identification of the species without analysis of more samples, populations and species. The means of within population genetic distances, based on Dice's and Jaccard's similarity indices, were similar for the rusa (0.383 and 0.542, respectively) and sika (0.397 and 0.558, respectively) populations with the sambar population being the least variable (0.194 and 0.323, respectively). The Dice based genetic distances within the species ranged from 0.194 to 0.397 and the genetic distances among the species were 0.791-0.911. The genetic distances based on Dice's and Jaccard's similarity indices between the rusa and sambar were 0.556 and 0.713, between the rusa and sika populations were 0.552 and 0.710, and between sambar and sika were 0.622 and 0.766, respectively.

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Section: Discussioncontrasting

confidence: 57%

Section: Discussionmentioning

confidence: 90%

RAPD Analysis of Three Deer Species in Malaysia

El-Jaafari¹,

Panandam²,

Idris³

et al. 2008

Asian Australas. J. Anim. Sci

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“…They consist of strings of tandem repeats of short motifs ranging from mono to penta nucleotides with variability in the number of repeats (Litt andLuty, 1989, Fan et al, 2005;Sukla et al, 2006). Highly variable STR loci are common in mammalian genomes and can readily be typed by Polymerase Chain Reaction followed by electrophoresis on denaturing ureapolyacrylamide gel (Sodhi et al, 2006). These attributes make microsatellites the ideal marker to reveal parentage/genetic relationships between individuals.…”

Section: Introductionmentioning

confidence: 99%

Production of Kids from In vitro Fertilized Goat Embryos and Their Parentage Assessment Using Microsatellite Markers

Malakar¹,

Das²,

Mukesh³

et al. 2007

Asian Australas. J. Anim. Sci

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The purpose of the present study was to produce live offspring from in vitro fertilized goat embryos. Oocytes were collected from abattoir ovaries and kept in oocyte collection medium. Oocytes were washed 4-5 times with maturation medium containing medium-199 with 5 µg/ml FSH, 100 µg/ml LH, 1 µg/ml estradiol-17β, 50 µg/ml gentamycin, 10% inactivated estrus goat serum, and 3% BSA (fatty acid free). Oocytes were placed in 100 µl drops of maturation medium containing granulosa cell monolayer and incubated in a 5% CO 2 incubator at 38.5°C for 27 h. For capacitation of spermatozoa fresh semen was processed and mixed in 3 ml fertilization TALP medium containing 50 µg/ml heparin and kept in the above incubator for 2 h. The capacitated spermatozoa were coincubated with matured oocytes for fertilization. Cleaved embryos were separated and cultured in embryo development medium with oviductal cells and 494 embryos were produced. Recipient goats were synchronized with two injections of 15 mg PGF 2α /goat 10 days apart. Eighty early stage embryos were transferred into the uterotubal junction of 14 surrogate mothers using laparoscopy techniques. One recipient delivered twin kids, whereas another two recipients each.delivered a single kid The parentage of these kids was evaluated using highly polymorphic co-dominant microsatellites markers. From the present study, it was concluded that live goat kids can be produced from in vitro matured and fertilized goat embryos, to the best of our knowledge for the first time in India.

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